Detection kit for detecting HCV (Hepatitis C) antibody in saliva and preparation method of detection kit

Abstract

The invention relates to a detection kit for detecting an HCV (Hepatitis C) antibody in saliva and a preparation method of the detection kit, and belongs to the technical field of biological detection. The detection kit comprises a clamp casing, a saliva pad and a test strip, wherein the test strip comprises a base plate, a sample pad, a gold-labelled pad, an NC membrane and absorbent paper, and the sample pad, the gold-labelled pad, the NC membrane and the absorbent paper are stuck on the base plate in sequence; the gold-labelled pad is coated with a colloidal gold marker; the NC membrane iscoated with an HCV recombinant antigen and a second antibody; the saliva pad and the sample pad are connected; the colloidal gold marker is a mixture of an HCV recombinant antigen-colloidal gold marker and a mouse IgG-colloidal gold marker or a binding protein proteinG-colloidal gold marker. According to the detection kit, saliva is acquired by the saliva pad, conveyed to the sample pad and subjected to immunoreaction with the colloidal gold marker on the gold-labelled pad and the recombinant antigen on the NC membrane by lateral chromatography, and a result visible to naked eyes is displayed.

Description

technical field [0001] The invention relates to a detection kit for detecting HCV antibody in saliva and a preparation method thereof, belonging to the technical field of biological detection. Background technique [0002] Viral hepatitis C, referred to as hepatitis C (Hepatitis C), hepatitis C, is a viral hepatitis caused by hepatitis C virus (HCV) infection, mainly through blood transfusion, acupuncture, drug abuse, etc., according to the World Health Organization According to statistics from organizations, the global HCV infection rate is about 2.8%. It is estimated that about 185 million people are infected with HCV, and about 350,000 deaths are caused by HCV infection every year. [0003] Due to the occult nature of HCV infection, most infected persons do not know that they are infected with HCV. During the incubation period, a large proportion of patients have no obvious symptoms. Therefore, timely and effective diagnosis of hepatitis C is very important. As a rapid d...

AI Technical Summary

Problems solved by technology

[0005] Most of the saliva detection reagents currently in the market use sponge sticks to take samples first, then mix them with diluent, and then add samples for testing after mixing. This detection method has the following shortcomings: 1. The saliva sampling process is cumbersome; 2. It is prone to dilution 3. Due to the different ways of adding samples, there are problems such as adding too much or not enough

The detection kit disclosed in this patent needs to first use the nylon pad end of the oral swab to take a sample from the gums of the mouth, then immerse the oral swab in the sample buffer, make the two sides of the swab close to the wall of the test tube successively, scrape up and down, and the inside of the tube The liquid is the oral fluid sample, and then the test strip is placed flat, and 100 μL of the sample is added to the sample pad for testing. The patented oral fluid sampling is cumbersome, and the solution is incompletely mixed with the buffer solution, which affects the test results.

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