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A kind of immunoprotective combination protein and immune vaccine thereof

A combination protein and immune protection technology, applied in the biological field, can solve the problems of high cost and poor effect

Active Publication Date: 2020-11-03
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment of cerebral echinococcosis usually includes drug therapy and surgical treatment. In the late stage of the disease, multi-purpose drug therapy is often ineffective, and the cost of surgical treatment is high

Method used

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  • A kind of immunoprotective combination protein and immune vaccine thereof
  • A kind of immunoprotective combination protein and immune vaccine thereof
  • A kind of immunoprotective combination protein and immune vaccine thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Effect of Tm16+TmGST protein on cerebral polycephalic cysts

[0034] About 1 to 2 weeks after the TmGST vaccine group and the saponin control group were orally challenged with the eggs of Taenia polycephala in Table 1, 1 and 3 sheep died respectively (see Table 2), and encephalitic lesions were found in the brain tissue after autopsy , At the same time, before death, he was accompanied by meningoencephalitis symptoms such as elevated body temperature, anorexia, and listlessness. 105 days (about 3.5 months) after oral infection with Taenia polycephali eggs, all the goats in each group were autopsyed, and cysts were found in all the goats in the control group. See Table 2 for the cyst infection in the brain of the vaccine group. It can be seen from Table 2 that in the saponin control group, the polycephalic cysts were all located in the brain and spinal cord of the goats, with an average diameter of about 3 cm, while in the combined immunization group, all the ...

Embodiment 2

[0043] Embodiment 2: Indirect ELISA detection of the serum antibody of Tm16+TmGST protein

[0044] Using indirect ELISA method, using rTm16 and rTmGST as immune antigens to detect specific IgG antibody in the goat serum of each immunized group (using rTm16 and rTmGST to detect respectively in the combined immunized group). 20. Specific antibody OD for each test group using SPSS 450nm values ​​for statistical analysis.

[0045] Using rTm16 and rTmGST proteins as detection antigens, the indirect ELISA method was used to detect serum-specific IgG, and the OD of serum IgG in each group was shown in Table 1 450nm For value changes see figure 2 . From figure 2 It can be seen that after the first immunization, the serum IgG values ​​of goats in the TmGST group, Tm16 group, and combined immunization group increased significantly. The third immunization was given half a year after the second immunization, and the specific IgG of each immunization group increased rapidly, among w...

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Abstract

The invention relates to the technical field of biology, and discloses immunoprotection combined protein and an immune vaccine thereof. The immunoprotection combined protein includes Tm16 protein andTm-GST protein, or is Tm16 and Tm-GST fusion protein. The Tm16 protein and the Tm-GST protein are combined to be used as an immunogen immune animal; the immunoprotection effect by aiming at optimum cysticercus cerebralis is generated on animals; the synergistic effect is achieved; higher specific antibody level can be induced; a novel measure for preventing and controlling cysticercus cerebralis diseases is formed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune protection combination protein and an immune vaccine thereof. Background technique [0002] Coenurus cerebralis is the middle-stage larva of Taenia multiceps, which often parasitizes the brain, spinal cord and other central nervous systems, subcutaneous and intermuscular parts of artiodactyl herbivores such as goats, sheep and cattle. Cerebral polycephalus mainly causes dysfunction of the host brain and central nervous system. The disease is common in developing countries with underdeveloped economies such as Africa and Southeast Asia, and brings huge economic losses to the herbivorous animal breeding industry. In my country, it is most common in vast pastoral areas such as Northwest China, North China, and Northeast China, as well as agricultural and pastoral areas in the south. In addition, people can also be accidentally infected with the disease, which has been report...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61K39/00A61P33/10
CPCA61K39/0003A61P33/10C07K14/4355C07K2319/00
Inventor 杨光友郭程
Owner SICHUAN AGRI UNIV
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