Content determination method of magnoflorine in dichocarpum sutchuenense medicinal material

A magnolidine and determination method technology, which is applied in the directions of measuring device, material separation, analysis of materials, etc., can solve problems such as difficulty in controlling the quality of medicinal materials and their formulations, restricting the development and utilization of herringbone fruit, and no quantitative detection of medicinal effect components, etc. question

Active Publication Date: 2018-10-23
贵州中医药大学
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Problems solved by technology

At present, there is no research report on the determination method of magnolia alkaloid in the medicinal material of citronella, which limits the further development and utilization of citronella
In addition, there is no quantitative detection of its medicinal ingredients, it is difficult to control the quality of medicinal materials and their prescriptions, and it is impossible to guarantee the safety and effectiveness of clinical medication

Method used

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  • Content determination method of magnoflorine in dichocarpum sutchuenense medicinal material
  • Content determination method of magnoflorine in dichocarpum sutchuenense medicinal material
  • Content determination method of magnoflorine in dichocarpum sutchuenense medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] A method for assaying the content of magnolanine in the herb fruit medicinal material, the method for assaying the content of magnolanine is:

[0076] Chromatographic conditions: Pntulips BP-C 18 Column (4.6mm×250mm, 2.5μm); mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid-0.1% triethylamine aqueous solution, gradient elution program: 0-10min, 5%-20% A, 10~30min, 20%~40%A; detection wavelength: 270nm; column temperature: 25°C; flow rate: 1mL min -1 ;Injection volume: 10μL;

[0077] Preparation of reference substance solution: Accurately weigh an appropriate amount of magnolialine reference substance, put it in a volumetric flask, add 75% methanol solution to make 0.40032 mg·mL -1 The reference substance solution;

[0078] Preparation of the test solution: take 1.0 g of herb powder, accurately weigh it, place it in a stoppered Erlenmeyer flask, add 25 mL of 75% methanol solution accurately, weigh it, and extract it ultrasonically fo...

Embodiment 2

[0081] A method for assaying the content of magnolanine in the herb fruit medicinal material, the method for assaying the content of magnolanine is:

[0082] Chromatographic conditions: Pntulips BP-C 18 Column (4.6mm×250mm, 2.5μm); mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid-0.1% triethylamine aqueous solution, gradient elution program: 0-10min, 5%-20% A, 10~30min, 20%~40%A; detection wavelength: 270nm; column temperature: 25°C; flow rate 0.8mL min -1 ;Injection volume 10μL;

[0083] Preparation of reference substance solution: Accurately weigh magnolialine reference substance, put it in a volumetric flask, add 80% methanol solution to make 0.50032 mg·mL -1 The reference substance solution;

[0084] Preparation of the test solution: take 1.0 g of herb powder, accurately weigh it, place it in a stoppered Erlenmeyer flask, add 20 mL of 50% methanol solution accurately, weigh it, and extract it ultrasonically for 30 min at a power of 10...

Embodiment 3

[0087] A method for assaying the content of magnolanine in the herb fruit medicinal material, the method for assaying the content of magnolanine is:

[0088] Chromatographic conditions: Pntulips BP-C 18 Column (4.6mm×250mm, 2.5μm); mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid-0.1% triethylamine aqueous solution, gradient elution program: 0-10min, 5%-20% A, 10~30min, 20%~40%A; detection wavelength: 280nm; column temperature: 30°C; flow rate: 1.2mL min -1 ;Injection volume 10μL;

[0089] Preparation of reference substance solution: Accurately weigh an appropriate amount of magnolialine reference substance, put it in a volumetric flask, add 75% methanol solution to make 0.40032 mg·mL-1 The reference substance solution;

[0090] Preparation of the test solution: Take about 1.2g of the herb powder, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50mL of 90% methanol solution accurately, weigh it, and extract it by ultrasoni...

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Abstract

The invention discloses a content determination method of magnoflorine in a dichocarpum sutchuenense medicinal material. The content determination method of magnoflorine is as follows: chromatographiccondition: Pntulips BP-C18 column (4.6 mm*250 mm, 2.5 microns); mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid- 0.1% triethylamine aqueous solution, gradient elution; detection wavelength: 260-280 nm; column temperature: 20-30 DEG C; flow velocity: 0.8-1.2 mL.min<-1>; and sample size: 5-20 microliters. According to the invention, magnoflorine is used as the determination index, a high performance liquid chromatography method is adopted, and a content determination method of the dichocarpum sutchuenense medicinal material is established, thus providing experimental bases for quality standard research of the dichocarpum sutchuenense medicinal material. The determination method is accurate, has high sensitivity, good repeatability and reliable results, and provides basis for quality control and evaluation of the dichocarpum sutchuenense medicinal material.

Description

technical field [0001] The invention relates to a method for determining the content of magnolanine in citronella medicinal material, which belongs to the field of pharmaceutical technology. Background technique [0002] The herb of Dichocarpum dalzielii (J.R.Drummond&Hutchinson) W.T.Wang&P.K.Hsiao is the dried whole plant of Dichocarpum dalzielii (J.R.Drummond&Hutchinson) W.T.Wang&P.K.Hsiao. and other diseases. In 2015, the Guizhou Food and Drug Administration established a project to conduct research on the revision of the 2003 edition of the "Quality Standards for Traditional Chinese and Ethnic Medicinal Materials in Guizhou Province", and listed Herringbone as a new tonic medicinal material, and carried out research on its quality standards. Through literature review and analysis, there are few research reports on Pteridocarpus frondosa, only taxonomic research, and research on its chemical composition and pharmacological effects is still blank. In this experiment, fir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 徐文芬柏彩红王波孙庆文李云超
Owner 贵州中医药大学
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