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Application of spermidine salt in prevention and treatment of pine wood nematode diseases

A technology of spermidine salt and spermidine phosphate, which is applied in application, nematicides, chemicals for biological control, etc., can solve the problems of high toxicity, environmental pollution, and high cost of use, and achieve simple preparation, Environmentally friendly and wide-ranging effects

Active Publication Date: 2018-10-23
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the control of pine wood nematode disease mostly adopts chemical control methods, such as the use of fensulfion, methimenphos, etc., but these pesticides are gradually restricted in application due to problems such as high toxicity, environmental pollution, and high cost of use. Has been banned from use, making the control of pine wood nematodes face great difficulties

Method used

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  • Application of spermidine salt in prevention and treatment of pine wood nematode diseases
  • Application of spermidine salt in prevention and treatment of pine wood nematode diseases
  • Application of spermidine salt in prevention and treatment of pine wood nematode diseases

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Embodiment 1

[0032] 1. Drug preparation

[0033] Spermidine trihydrochloride and spermidine phosphate solution preparation: Spermidine trihydrochloride and spermidine phosphate were purchased from Sigma (Spermidine trihydrochloride, item number: S2501, CAS number: 334-50-9 or Spermidine phosphatesalt hexahydrate, item number : S0381, CAS No.: 23273-82-7).

[0034] Weigh 1 gram of spermidine salt with an electronic balance, and place it in a 15 mL centrifuge tube, add 4 mL of sterilized ultrapure water into the tube, dissolve at room temperature in the dark, and obtain a final concentration of 250 mg / mL. Aqueous solution of spermidine trihydrochloride and spermidine phosphate.

[0035] Then use sterilized ultrapure water to dilute the spermidine trihydrochloride and the spermidine phosphate aqueous solution to a series of dilution solutions respectively by means of gradient dilution.

[0036] 2. Experimental method

[0037] Take 12-well plates respectively, add 400 μL sterilized ultrapur...

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Abstract

The invention discloses an application of spermidine salt in prevention and treatment of pine wood nematode diseases. For the first time, it is found that spermidine salts, such as spermidine trihydrochloride and spermidine phosphate, have significant poisoning killing effect against bursaphelenchus nematodes, such as pine wood nematodes, and can be applied to prevention and treatment of the pinewood nematodes and other bursaphelenchus nematodes. In addition, the spermidine trihydrochloride and spermidine phosphate can be prepared through reactions to spermidine with hydrochloric acid and phosphoric acid, so that the spermidine salt is simple in preparation approach and has better environmental compatibility, is safe and high-effective, can be used for developing more safe and high-effective novel pine wood nematode prevention and treatment reagents, has important theoretical guidance significance and practical application value in prevention and treatment on plant diseases caused bythe pine wood nematodes and the like bursaphelenchus nematodes, and has great promotion and application prospect.

Description

technical field [0001] The invention belongs to the technical field of prevention and control of agricultural and forestry diseases. More specifically, it relates to the application of spermidine salt in the control of pine wood nematode disease. Background technique [0002] Pine xylophilus ( Bursaphelenchus xylophilus ) Belonging to the order of the order of the genus, the family of the genus, the genus of the genus ( Bursaphelenchus Fuchs), is a destructive pest on pine plants, and the pine wood nematode disease caused by it is also called pine wilt, which is known as "pine cancer". After the onset of the disease, the plants die quickly, and the transmission speed is extremely fast. Once the disease occurs, it will cause extremely serious losses. Pine wood nematode disease has been listed as a quarantine object by many countries. The disease was first discovered in Sun Yat-sen's Mausoleum in Nanjing in 1982 in my country. At present, the disease has spread to many pr...

Claims

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Application Information

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IPC IPC(8): A01N33/04A01P5/00
CPCA01N33/04
Inventor 卓侃林柏荣李君霞廖金铃
Owner SOUTH CHINA AGRI UNIV
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