Full-optical fiber type super-resolution imaging method and device

Abstract

The invention discloses a full-optical fiber type super-resolution imaging method and device. The full-optical fiber type super-resolution imaging method comprises the following steps: combining excited light and Gauss loss light into one beam, and conducting the beam into a raster type optical fiber light vortex converter through a light vortex optical fiber; converting the Gauss loss light intolight vortex loss light from the beam by using the raster type optical fiber light vortex converter, and maintaining spatial distribution of the excited light; focusing the light vortex loss light andthe excited light to a sample through an optical fiber micro-lens; controlling the intensities of the excited light and the Gauss loss light, and acquiring fluorescent excitation smaller than a diffraction limit; collecting the excited fluorescent light through the optical fiber micro lens; exploiting intensities of corresponding excited fluorescence in different positions of the sample, therebyobtaining super-resolution images of the sample. By adopting the method, by improving the flexibility and the integration of a conventional STED (Super-Resolution Imaging Device), the STED can be applied to endoscopy super-resolution imaging of a microscopic scale.

Description

technical field [0001] The present invention relates to the technical field of photonics, and more specifically, to an all-fiber super-resolution imaging method and device. Background technique [0002] Stimulated emission depletion (STED) imaging utilizes the nonlinear relationship between fluorescence saturation and excited-state fluorescence stimulation loss. Fluorescent emission range, compressing effective point spread function, improving system resolution. A typical STED system has two strictly coaxial laser illumination beams, one of which is excitation light and the other is depletion light (also called stimulated emission depletion light). The excitation light generally has a Gaussian distribution, and after focusing, a diffraction-limited focal spot (Airy disk) is obtained. Lost light has an annular light intensity distribution similar to a doughnut, and the light intensity at the center of the beam is zero after focusing. Ring-loss light generally adopts optica...

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Problems solved by technology

[0006] Aiming at the defects of the prior art, the purpose of the present invention is to solve the problem that the existing optical fiber stimulated emission loss system is not an all-fiber structure, but uses a part of free space optical path and part of optical fiber transmission, and its stability, anti-interference ability and flexibility And the degree of integration is not optimal, and the microlens size (millimeter level) used in the current optical fiber STED endoscopy system is still relatively large, and it is impossible to penetrate into some biological tissues and organs to realize the technical problem of endoscopic imaging under micron gaps

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