Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New method for rapid label-free detection of cysteine

A technology of cysteine ​​and a new method is applied in the field of standard-free rapid detection of cysteine, which can solve the problems of influence of the accuracy of detection results, increase of detection cost, easy failure of hydrogen peroxide, etc., and achieves low cost, simple method, and easy operation. simple effect

Active Publication Date: 2018-10-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
View PDF12 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, we have noticed that although there are many literature reports on the colorimetric detection method of cysteine ​​(Talanta 2016, 82, 71-74.; Anal. Methods 2014, 6, 5647-5651.; Sensors and Actuators B: Chemical 2015, 212, 526-535.; Analyst 2015, 140, 5251-5256.; Chem.Commun.2015, 51, 4635-4638.), but most of them are based on the presence of hydrogen peroxide
As we all know, hydrogen peroxide is prone to failure and is not easy to store. It usually needs to be prepared immediately after use. The use of hydrogen peroxide not only increases the cost of testing, but also has a certain impact on the accuracy of test results.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New method for rapid label-free detection of cysteine
  • New method for rapid label-free detection of cysteine
  • New method for rapid label-free detection of cysteine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1.17 μM TMB (a); 1.17 μM TMB mixed with 30 μM Cys (b); 1.17 μM TMB mixed with 17 μg / mL Co-CNMs (c); 1.17 μM TMB, 17 μg / mL Co-CNMs mixed with 30 μM Cys (d), utilize ultraviolet spectrophotometer to measure TMB ultraviolet absorption response (such as figure 1 shown). figure 1 It shows that the Co-CNMs we synthesized have excellent catalytic properties, which can directly catalyze TMB to turn blue, and the color turns yellow after being terminated with sulfuric acid, and the addition of cysteine ​​can inhibit the catalytic activity of Co-CNMs, thereby causing TMB to absorb ultraviolet light. decrease in value.

Embodiment 2

[0024] 1.17μM TMB was mixed with 17.0μg / mL Co-CNMs and incubated with MES buffer with different pH values ​​(3.0, 4.0, 5.0, 6.0, 7.0) to measure the UV absorption response of TMB. Take the maximum absorption peak at the wavelength of 450nm as the ordinate, and the pH value as the abscissa to make a point-line diagram. At pH 4.0, the sensing system absorbs the highest value, so the detection system is carried out at pH 4.0.

Embodiment 3

[0026] 17μg / mL Co-CNMs was mixed with different concentrations of TMB (0.5, 0.67, 0.83, 1.0, 1.17, 1.3mM), and the UV absorption response of TMB was measured. Take the maximum absorption peak at the wavelength of 450nm as the ordinate, and TMB with different concentrations as the abscissa to make a histogram. When the concentration of TMB reached 1.2mM, the absorption response of the sensing system appeared the highest value, and then no longer changed with the increase of TMB concentration, so 1.2mM was selected as the concentration of TMB in the sensing system.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a new method for rapid label-free detection of cysteine (Cys), and the method is mainly based on detection of changes in UV absorbance value of 3,3',5,5'-tetramethylbenzidine (TMB) caused by addition of the cysteine in the 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of cobalt-doped carbon nanomaterials (Co-CNMs). The TMB usually changes in color in the presence ofhydrogen peroxide. The synthesized Co-CNMs have excellent catalytic properties, and can directly catalyze the TMB to change into blue, after sulfuric acid is used to stopping, the color turns yellow,the catalytic activity of the Co-CNMs can be inhibited by the addition of the cysteine, decrease in the UV absorbance value of the TMB is caused, and the decrease in the UV absorbance value of the TMBis linearly related to the concentration of the added cysteine. The hydrogen peroxide is easy to decompose and unstable in nature. The method avoids the use of the hydrogen peroxide and can greatly improve the accuracy of test results. The method is very simple, has strong specificity, good stability and fast detection speed, and can meet the requirements of rapid and accurate on-site detection.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a new method for label-free rapid detection of cysteine ​​(Cys), which belongs to the technical field of detection. technical background [0002] Cysteine ​​is an important thiol-containing amino acid in the human body. Due to the high nucleophilicity of the sulfhydryl group it contains, cysteine ​​is an important active species for life processes in organisms, it can regulate the activity of proteins in organisms and play a role in the intracellular antioxidant defense system played a vital role. The level of cysteine ​​content in the living body can provide a scientific basis for the diagnosis of some diseases. For example, the reduction of cysteine ​​content in the body will lead to slow growth of infants, lung dysfunction, etc.; and higher cysteine ​​content is closely related to the occurrence of some cancers and cardiovascular diseases. Therefore, the detection of cys...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 刘亚青钱芃橙王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com