Method for simultaneously extracting various kinds of pueraria isoflavones glycoside from radix puerariae crude extract

A technology of pueraria isoflavone glycosides and crude extracts, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve problems such as large pollution and high cost, and achieve simple operation, low cost and environment-friendly. Effect

Active Publication Date: 2018-10-12
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses a large amount of toxic and harmful chemical solvents, causing great pollution, and uses normal phase silica gel and reverse phase silica gel at the same time, but only one kind of product is obtained, and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Impurity removal: Add 200kg of ice water at 0°C to 50kg of Pueraria lobata crude extract, stir for 60min, and centrifuge to obtain a filter cake;

[0044] (2) Alumina sample mixing: first disperse the filter cake obtained in step (1) with 25kg of ethanol, then add 50kg of alumina, stir evenly, and dry in vacuum to obtain sample alumina;

[0045] (3) Alumina filling: first add 400kg of fresh alumina to the chromatography column, and then fill the mixed sample alumina obtained in step (2) on it, and the aspect ratio of the alumina column is 5:1;

[0046] (4) Gradient elution: Use the following gradient eluents sequentially, at a flow rate of 1BV / h, to perform gradient elution on the alumina column in step (3): Gradient I, the eluent is ethanol aqueous solution with a volume fraction of 10%, The dosage is 4BV; for gradient II, the eluent is 20% ethanol aqueous solution, and the dosage is 4BV; for gradient III, the eluent is 65% ethanol aqueous solution, and the dosage ...

Embodiment 2

[0049] (1) Impurity removal: Add 125kg of ice water at 5°C to 25kg of Pueraria lobata crude extract, stir for 40min, and centrifuge to obtain a filter cake;

[0050] (2) Silica gel sample mixing: first disperse the filter cake obtained in step (1) with 12.5kg of methanol, then add 25kg of silica gel, stir evenly, and dry in vacuum to obtain the sample silica gel;

[0051] (3) Silica gel filling: first add 150kg of fresh silica gel to the chromatography column, and then fill the mixed sample silica gel obtained in step (2) on it, and the height-to-diameter ratio of the silica gel column is 6:1;

[0052] (4) Gradient elution: Use the following gradient eluents sequentially at a flow rate of 0.5BV / h to perform gradient elution on the silica gel column in step (3): Gradient I, the eluent is 15% methanol aqueous solution, The dosage is 3BV; for gradient II, the eluent is 25% methanol aqueous solution, and the dosage is 3BV; for gradient III, the eluent is 55% methanol aqueous solut...

Embodiment 3

[0055] (1) Impurity removal: add 60kg of ice water at 5°C to 20kg of Pueraria lobata crude extract, stir for 30min, and centrifuge to obtain a filter cake;

[0056] (2) Polyamide resin sample mixing: first disperse the filter cake obtained in step (1) with 15 kg of ethanol, then add 30 kg of polyamide resin, stir evenly, and dry in vacuum to obtain the mixed sample polyamide resin;

[0057] (3) Polyamide resin filling: first add 100kg of fresh polyamide resin to the chromatography column, and then fill the mixed sample polyamide resin obtained in step (2) on it. The height-to-diameter ratio of the polyamide resin column is 4 :1;

[0058] (4) Gradient elution: Use the following gradient eluents sequentially, at a flow rate of 1.5BV / h, to perform gradient elution on the polyamide resin column in step (3): Gradient I, eluent is ethanol with a volume fraction of 15% Aqueous solution, the dosage is 4BV; Gradient II, the eluent is 30% ethanol aqueous solution, the dosage is 4BV; Gr...

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Abstract

The invention provides a method for simultaneously extracting various kinds of pueraria isoflavones glycoside from a radix puerariae crude extract. The method comprises the following steps that (1) ice water is added into the radix puerariae crude extract, stirred and centrifugally filtered, and a filter cake is obtained; (2) the filter cake is dispersed by using lower alcohol, then a filling agent is added and stirred to be uniform, vacuum drying is conducted, and a stirring sample filling agent is obtained; (3) a fresh filling agent is added into a chromatographic column, and then the stirring sample filling agent is filled onto the chromatographic column; and (4) an eluent with the concentration being incremental is utilized to conduct gradient elution, eluant is collected and subjectedto vacuum concentration, cooling, stirring crystallizing, centrifugal filtration and drying, and genistin, daidzin and puerarin are obtained respectively. The content of genistin obtained by adoptingthe method is as high as 98.7%, and the yield is as high as 92%; the content of daidzin is as high as 98.5%, and the yield is 92%; and the content of puerarin is as high as 99.9%, and the yield is 97%. The method is easy to operate, low in cost, environmentally friendly, and suitable for industrialized production.

Description

technical field [0001] The invention relates to a method for simultaneously extracting multiple kinds of pueraria isoflavone glycosides, in particular to a method for simultaneously extracting multiple kinds of pueraria isoflavone glycosides from pueraria root crude extract. Background technique [0002] Pueraria lobata is the tuberous root of the leguminous plant Pueraria mirifica or Gange vine, mainly produced in Henan, Hunan, Zhejiang, Sichuan and other places. It has the functions of relieving muscle and reducing fever, promoting body fluid, promoting rash, promoting yang and antidiarrheal. Wild kudzu root contains 12% flavonoids, such as puerarin, daidzin, peanut and other nutrients, as well as protein, amino acid, sugar, isoflavone glycosides and minerals such as iron, calcium, copper and selenium necessary for human body. It is a precious tonic suitable for all ages, and has the reputation of "thousand-year ginseng". Among them, isoflavone glycosides have good nutri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 李伟龙伟岸黄华学
Owner HUNAN HUACHENG BIOTECH
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