Method for performing immunochromatographic detection on escherichia coli O157:H7 by taking colored silicon dioxide nanometer microspheres as markers

Abstract

The invention discloses a method for performing immunochromatographic detection on escherichia coli O157:H7 (E.coli O157:H7) by taking colored silicon dioxide nanometer microspheres as markers. The colored silicon dioxide nanometer microspheres colored SiNPs are prepared by taking active dye as a dyeing agent; in a system (the formula is as shown in the description), carboxyl is modified on the surface of the colored SiNPs through carboxyl ethyl silantriol sodium salt, and the carboxyl-modified colored SiNPs are obtained; an immune core-housing type silicon dioxide nanoparticles colored SiNPs-mAb1 are prepared by fixing a monoclonal marker antibody mAb1 of anti-mouse E.coli O157:H7 on the surface of the E.coli O157:H7; a detection line of an NC membrane is coated with an anti-mouse E.coliO157:H7 monoclonal capture antibody mAb2 and a quality control line is coated with goat anti-mouse IgG; and the generated compounds are dropwise added into a sampling hole of a test paper strip by a method for mixing a pathogenic bacterial sample and colored SiNPs-mAb1 in advance to form a sandwich structure between a T line and the Mb2 to realize rapid detection of the E.coli O157:H7. The methodprovided by the invention has high detection sensitivity and specificity, is simple and time-saving, is environment-friendly and economic in process, and has a great application prospect.

Description

technical field [0001] The invention relates to an immunochromatographic detection technology, in particular to an immunochromatographic detection method of Escherichia coli O157:H7 with colored silicon dioxide nanometer microspheres as markers. Background technique [0002] Escherichia coli O157H7 (E.coli O157:H7) is the main serotype of Enterohemorrhagic escherichia coli (EHEC), which can cause diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombosis Thrombocytopenic purpura and other diseases. Most of the epidemic outbreaks of this bacterium are related to eating food contaminated by this bacterium, and other pollution routes include drinking water and air. Since the discovery of E.coli O157:H7 in 1982, there have been poisoning incidents all over the world, posing a threat to human health. Therefore, it is of great significance to establish a fast, simple and specific detection method for the prevention and control of E.coli O157:H7. [0003] According ...

AI Technical Summary

Problems solved by technology

Fluorescent nanomaterials require special instruments, and signaling markers such as liposomes and enzymes are expensive, limitations that limit their widespread use in rapid assays

So far, the most commonly used markers are still AuNPs, but AuNPs are easily affected by acid-base, temperature and salt concentration, and have a single color, which limits their application in multiple detection; in addition, the combination of AuNPs particles and biomacromolecules The process is less stable in the liquid phase and prone to aggregation

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