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Pichia membranifaciens for controlling diseases after peach harvesting

A technology of Pichia membranaceus and postharvest is applied in the field of postharvest disease biological control of fruits, which can solve the problems of polluting the environment, endangering human health, drug resistance of pathogenic bacteria, etc., so as to reduce economic losses, avoid harm to human body, and significantly improve the The effect of economic and social benefits

Active Publication Date: 2018-10-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical fungicides have been widely used for a long time because of their advantages of high efficiency, low price, and convenient use. However, this method has the disadvantages of causing drug resistance of pathogenic bacteria, endangering human health and polluting the environment.

Method used

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  • Pichia membranifaciens for controlling diseases after peach harvesting
  • Pichia membranifaciens for controlling diseases after peach harvesting
  • Pichia membranifaciens for controlling diseases after peach harvesting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: The microbiological characteristics of Y4 bacterial strain are as follows:

[0021] 1) Morphological features

[0022](1) Cultivate on NYDA medium at 28°C for 2-3 days, the colony is round, milky white, slightly raised, with smooth edges, moist and easy to stir up. The cell shape is oval or oval, with budding at one or both ends.

[0023] (2) After being cultured in NYDB liquid medium for 24 hours, the bacterial solution was turbid, precipitated and had a special smell. After standing in the NYDB liquid medium for 3 days, it can form a mold and produce air bubbles.

[0024] 2) Molecular biological identification

[0025] Sequence analysis of the 5.8S rDNA-ITS region of the isolated and screened yeast strain Y4 was retrieved in GenBank and determined to be Pichia membraneefaciens. According to the retrieved homologous strains, the Mege5.1 program of DNAStar software was used to construct the biological evolutionary relationship tree as follows: figure ...

Embodiment 2

[0027] Embodiment 2: safety experiment of Pichia membranosa Y4

[0028] 1) Experimental scheme

[0029] Preparation of yeast sludge: Inoculate Pichia membranosum Y4 into a 250ml Erlenmeyer flask filled with 50ml NYDB medium, culture on a shaker at 28°C for 20 hours, centrifuge to obtain the sludge, wash and dilute with sterile water to obtain the required concentration. Each time the mice were gavaged with bacteria solution, they were diluted with fresh bacteria sludge.

[0030] The test species is ICR mice, which were purchased from the Animal Experiment Center of Jiangsu University with the certificate number No. 201613561, the license number SCXK (Su) 2013-0011, and the implementation standard GB15193.3-1994. Select 40 clean-grade ICR mice with body weight ranging from 18g to 22g, half male and half male. Before the test, the mice were placed in the animal experiment center and fed normally for 3 days, and fasted for 4 hours before the test. The mice were divided into 4 ...

Embodiment 3

[0036] Embodiment 3: the control effect of Pichia membranosum Y4 on peach soft rot

[0037] 1) Experimental scheme

[0038] Pichia membranii Y4 was inoculated in a Erlenmeyer flask containing NYDB medium, cultured in a shaker at 180rpm and 28°C for 20h, centrifuged to obtain the bacteria, resuspended with sterile water after washing and adjusted the concentration to 1×10 8 cells / mL. Cultivate Rhizopus glucoides on PDA medium at 28°C for 5-7d, and prepare the concentration of 1×10 with sterile water 5 spores / mL of spore suspension.

[0039] The variety tested was "Baifeng" peaches grown in the Green Ecological Base of Jurong City, Jiangsu Province. The peaches were not sprayed with any chemical pesticides. When picking, select peaches with no obvious bacterial infection or damage on the surface, fruit maturity and size and other related indicators that are basically the same. The fruit maturity is seven to eight mature. Three evenly distributed wounds (5mm×3mm) with the sam...

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Abstract

The invention discloses pichia membranifaciens Y4 for controlling diseases after peach harvesting and belongs to the field of biological prevention and control of diseases after fruit harvesting. Theyeast is identified as pichia membranifaciens, and the preservation number is CCTCC NO:M 2018110. The use method comprises the following steps: during the use, cultivating the pichia membranifaciens Y4 in an NYDA culture medium at 28 DEG C for 2 to 3 days, activating, performing fermentation cultivation in the NYDB culture medium at 28 DEG C for 20 to 24 hours, repeating the activation process fortwice, centrifuging to obtain thallus, washing for twice and diluting with sterile water to prepare bacterial suspension; perforating the peaches, adding the bacterial suspension and inoculating rhizopus stolonifer spore suspension after 2 hours; and airing at a room temperature, putting into a disinfected plastic basket, sealing with a preservative film, putting into a constant-temperature culture box and storing. Soft rot morbidity and decayed diameter of juicy peaches treated by the yeast are obviously reduced. The pichia membranifaciens Y4 can be applied to prevention and control of the juicy fruit soft rot, can reduce the use of chemical sterilizers and avoids danger to people and environmental pollution caused by the use of the chemical sterilizers.

Description

technical field [0001] The invention relates to a strain of Pichia membraneaefaciens Y4, which can control peach postharvest soft rot and belongs to the field of biological control of fruit postharvest diseases. Background technique [0002] Peach, scientific name Prunuspersica, Rosaceae, peach plant, is an excellent variety in peach fruits, is one of the main economic fruits in my country, has huge agricultural economic value, and is very important for improving the income of farmers in our country and promoting the development of local economy and industry is of great significance. Juicy peaches are deeply loved by consumers because of their beautiful appearance, bright colors, juicy meat, sweet taste and high nutritional value, and they have strong competitiveness in the sales market. Peach has high protein and mineral content, is rich in various vitamins and essential amino acids needed by the human body, has high edible and medicinal nutritional value, and contains huge...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/84
CPCA23B7/155A23V2002/00C12N1/165C12R2001/84A23V2200/10A23V2250/76
Inventor 张红印吴锋张晓云顾香玉赵利娜任晓锋
Owner JIANGSU UNIV
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