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Wilt-resistant PHD transcription factor ClPHD23 as well as gene, expression vector, transformant and application thereof

A fusarium wilt resistance and transcription factor technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2021-04-23
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the identification and regulation mechanism of watermelon Fusarium wilt resistance genes

Method used

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  • Wilt-resistant PHD transcription factor ClPHD23 as well as gene, expression vector, transformant and application thereof
  • Wilt-resistant PHD transcription factor ClPHD23 as well as gene, expression vector, transformant and application thereof
  • Wilt-resistant PHD transcription factor ClPHD23 as well as gene, expression vector, transformant and application thereof

Examples

Experimental program
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Effect test

experiment example

[0118] Cloning and functional verification of experimental example, transcription factor ClPHD23 gene of the present invention

[0119] The specific experimental steps are as follows:

[0120] 1. Cloning of watermelon PHD transcription factor ClPHD23 gene

[0121] The watermelon high-generation inbred line 'JJZ' preserved in our laboratory was planted in the greenhouse at the base of the Zhejiang Academy of Agricultural Sciences, and the watermelons that had grown to the fruit-setting stage were selected, and their roots, stems, leaves, male and female flowers that opened on the same day, and The pollinated fruit with a diameter of about 3 cm was quickly placed in liquid nitrogen, and then placed in a -80°C refrigerator until RNA extraction. The RNA extraction kit from TAKALA company was used to extract RNA, and the specific steps were referred to the instructions (TAKALA, Japan). The synthesis of the first strand of cDNA was carried out according to the instructions of the ...

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PUM

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Abstract

The invention discloses a wilt-resistant PHD transcription factor ClPHD23 as well as a gene, an expression vector, a transformant and application thereof, and belongs to the field of plant genetic engineering. The amino acid sequence of the wilt-resistant PHD transcription factor ClPHD23 is shown as SEQ ID NO.1. The wilt-resistant PHD transcription factor ClPHD23 provided by the invention can effectively regulate and control the resistance of plants to wilt, so that the onset time is delayed, and the onset degree is reduced.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a fusarium wilt-resistant PHD transcription factor ClPHD23, its gene, expression vector, transformant and application. Background technique [0002] Watermelon [Citrullus lanatus (Thunb.) Matsum & Nadai] belongs to the annual vine plant of Cucurbitaceae Watermelon. It is native to Africa and is an important economic crop. It is widely planted and eaten all over the world, but it is extremely vulnerable to The damage of various biotic and abiotic stresses has caused its quality and yield to be greatly reduced, which has greatly increased the planting cost of watermelons and seriously restricted the production and supply of watermelons. [0003] Wherein, Fusarium wilt pathogen belongs to Deuteromycotina, Fusarium oxysporum, Fusarium oxysporum f.sp.niveum (E.F.Smith) synder et Hansen). There are many specialized types in Fusarium oxysporum, and there are 5 specialized types t...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N1/21A01H5/00A01H6/34C12R1/01
CPCC07K14/415C12N15/8282
Inventor 何艳军范敏姚依秀李玉林张慧青
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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