Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A fusarium wilt resistant phd transcription factor clphd23, its gene, expression vector, transformant and application

A technology for resistance to fusarium wilt and transcription factors, which is used in applications, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2022-04-19
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the identification and regulation mechanism of watermelon Fusarium wilt resistance genes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A fusarium wilt resistant phd transcription factor clphd23, its gene, expression vector, transformant and application
  • A fusarium wilt resistant phd transcription factor clphd23, its gene, expression vector, transformant and application
  • A fusarium wilt resistant phd transcription factor clphd23, its gene, expression vector, transformant and application

Examples

Experimental program
Comparison scheme
Effect test

experiment example

[0118] Cloning and functional verification of experimental example, transcription factor ClPHD23 gene of the present invention

[0119] The specific experimental steps are as follows:

[0120] 1. Cloning of watermelon PHD transcription factor ClPHD23 gene

[0121] The watermelon high-generation inbred line 'JJZ' preserved in our laboratory was planted in the greenhouse at the base of the Zhejiang Academy of Agricultural Sciences, and the watermelons that had grown to the fruit-setting stage were selected, and their roots, stems, leaves, male and female flowers that opened on the same day, and The pollinated fruit with a diameter of about 3 cm was quickly placed in liquid nitrogen, and then placed in a -80°C refrigerator until RNA extraction. The RNA extraction kit from TAKALA company was used to extract RNA, and the specific steps were referred to the instructions (TAKALA, Japan). The synthesis of the first strand of cDNA was carried out according to the instructions of the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention "an anti-fusarium wilt PHD transcription factor ClPHD23, its gene, expression vector, transformant and application" belongs to the field of plant genetic engineering. The amino acid sequence of the Fusarium wilt-resistant PHD transcription factor ClPHD23 is shown in SEQ ID NO.1. The fusarium wilt-resistant PHD transcription factor ClPHD23 provided by the invention can effectively regulate the resistance of plants to the fusarium wilt, so as to delay the onset time and reduce the onset degree.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and relates to a fusarium wilt-resistant PHD transcription factor ClPHD23, its gene, expression vector, transformant and application. Background technique [0002] Watermelon [Citrullus lanatus (Thunb.) Matsum & Nadai] belongs to the annual vine plant of Cucurbitaceae Watermelon. It is native to Africa and is an important economic crop. It is widely planted and eaten all over the world, but it is extremely vulnerable to The damage of various biotic and abiotic stresses has caused its quality and yield to be greatly reduced, which has greatly increased the planting cost of watermelons and seriously restricted the production and supply of watermelons. [0003] Wherein, Fusarium wilt pathogen belongs to Deuteromycotina, Fusarium oxysporum, Fusarium oxysporum f.sp.niveum (E.F.Smith) synder et Hansen). There are many specialized types in Fusarium oxysporum, and there are 5 specialized types t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N1/21A01H5/00A01H6/34C12R1/01
CPCC07K14/415C12N15/8282
Inventor 何艳军范敏姚依秀李玉林张慧青
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com