Spore production device simulating wild environment and application thereof to antrodia camphorata

An environment and spore technology, which is applied in biochemical cleaning devices, enzymology/microbiology devices, and methods based on microorganisms, can solve problems such as insufficient culture conditions, accelerated aging, and low spore count, so as to shorten the spore formation time, Prevents bacterial contamination and enhances biological activity

Active Publication Date: 2018-10-02
ZHEJIANG HISUN PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of sporulation of Antrodia Cinnamomea is to isolate Antrodia Cinnamomea from wild Cinnamomum camphora, transfer it to a plate medium, and then transfer it to a liquid medium for shaking culture in a shaker flask. This process is prone to contamination by miscellaneous bacteria and the culture conditions are limited (including the reproduction and aging of bacteria and biologically active components. Content reduction), unable to increase the number of spores during the fermentation of Antrodia camphorata and easily cause aging
In particular, plate culture takes a long time, and the number of spores formed is relatively small, and its activity is relatively low; on the other hand, the number of spores is higher than that of plate culture in liquid shaking culture, but due to the rapid growth of spores and insufficient culture conditions , also accelerate aging, not suitable for mass production of Antrodia spores

Method used

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  • Spore production device simulating wild environment and application thereof to antrodia camphorata
  • Spore production device simulating wild environment and application thereof to antrodia camphorata
  • Spore production device simulating wild environment and application thereof to antrodia camphorata

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Experimental program
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Effect test

Embodiment 1

[0045] A sporulation device simulating a wild environment, the device includes a sporulation tank 1, a wooden block placement frame 3, a sterile air diffusion plate 2 and a spore collection plate 4, the upper end of the sporulation tank 1 is provided with a sealing cover 10, the The wooden block placement frame 3 is set in the sporulation tank 1, and the both sides of the wooden block placement frame 3 are respectively provided with a sterile air diffusion plate 2 and a spore collection plate 4; the sporulation tank 1 is provided with a nutrient solution Drip inlet 7, sterile air system inlet 5, spore outlet 6; the sterile air diffusion plate 2 communicates with the sterile air system inlet 5, and the sterile air system inlet 5 is provided with an air control valve 12. The spore collection plate 4 communicates with the spore output port 6; the spore output port 6 is provided with a pressure control valve 13, the nutrient solution droplet inlet 7 communicates with the nutrient s...

Embodiment 2

[0048] 1) Place the wheat stalk peptide wooden block in the wooden block placement frame. The preparation method of the wheat stalk peptide wooden block is as follows: 100g of wheat stalk powder, 50g of barley extract, 50g of corn gluten powder, 20g of malt extract and 20g of maltodextrin, add 760g of water, mix in the wood block forming mold, sterilize and cool to form;

[0049] 2) The nutrient solution input system is filled with sterilized liquid medium, the mass fraction of which is as follows: 2% barley extract, 5% maltose and 10% glucose, and the balance is water;

[0050] 3) In the ultra-clean workbench, the native bacteria of Antrodia cinnamomea (CCRC35396) were implanted into the sterilized wood block of wheat stalk peptide, so that the surface of wheat stalk peptide adsorbed bacteria solution on average to 2×10 7 CFU / cm 2 , and move it into the wooden box of the spore-producing tank, adjust the air control valve on the inlet of the sterile air system so that the ste...

Embodiment 3

[0051] Embodiment 3 (the embodiment of traditional preparation Antrodia camphorata spore)

[0052] First, take 1 ml of Antrodia antrodia fungus (CCRC35396) solution and drop it into the traditional MEA plate culture medium, and then culture Antrodia camphorata on the plate for 7 to 14 days at 28°C, then use an inoculation loop to pick out the Antrodia camphorata mycelium and put it into the MEB liquid culture medium for shaking Cultivate for 5-10 days. After measuring the number of spores, dilute it with sterilized MEB culture solution until the number of spores is 10 6 CFU / ml, spare.

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Abstract

The invention relates to a spore production device simulating wild environment and the application thereof to antrodia camphorata. The device comprises a spore production tank, a wood block laying basket, a sterile air diffusion plate and a spore collection plate, wherein a sealing cover, a temperature control system access port and a humidifying system access port are formed in the upper end of the spore production tank; the wood block laying basket is arranged in the spore production tank; the sterile air diffusion plate and the spore collection plate are respectively arranged on the two sides of the wood block laying basket; a nutrient solution instilling opening, a sterile air system inlet and a spore outlet are formed in the spore production tank. The spore production device can accelerate the reproduction of original spore, and can greatly shorten the spore formation time and prevent microbial contamination, meanwhile is good in activity of spore obtained through the device, andcan be applied to the reproduction of various fungi (such as antrodia camphorata, cordyceps sinensis and phellinus igniarius) spore, shorten the culture time and improve the biological activity.

Description

technical field [0001] The invention relates to a sporulation device and its application, in particular to a sporulation device simulating a wild environment and its application in Antrodia camphorata. Background technique [0002] At present, the spore production method of fungi is mainly to form spores naturally within a certain period of time by means of liquid shaking culture. This process easily leads to senility and death of spores after subsequent reproduction, and cannot increase the number and activity of spores. [0003] Antrodia Cinnamomea is rich in polysaccharides and triterpenoids. Among them, anti-oxidation, anti-inflammation, anti-tumor and immunity-enhancing effects and related biological active ingredients have been continuously researched and discovered. The cultivation technology and the acquisition and reproduction of wild spores are getting better and better. has received increasing attention from scholars. At present, the method of sporulation of Antr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/26C12M1/36C12N3/00C12R1/645
CPCC12M33/12C12M41/12C12M41/34C12N3/00
Inventor 江沛杜敏娜郑晓霞阮梦雅杨猷建滕云郑玲辉白骅其他发明人请求不公开姓名
Owner ZHEJIANG HISUN PHARMA CO LTD
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