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Specific polypeptide targeting at human colon cancer cells

A specific technology for human colon cancer cells, applied in the field of biomedicine, can solve the problems of patient treatment impact, large toxic and side effects, and large dosage of drugs, and achieve the effect of obvious effect, simple operation, and reliable scientific basis

Active Publication Date: 2018-10-02
中国医科大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Existing anticancer drugs usually have disadvantages such as high toxicity and side effects, large drug dosage, and easy to produce acquired drug resistance, which will affect the treatment of patients

Method used

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  • Specific polypeptide targeting at human colon cancer cells
  • Specific polypeptide targeting at human colon cancer cells
  • Specific polypeptide targeting at human colon cancer cells

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Experimental materials

[0023] 1.1 Phage peptide library, cells and host bacteria.

[0024] Phage 12 peptide library, Escherichia coli E.coli ER2738, human colon cancer SW480 cells, human normal intestinal epithelial HIEC cells.

[0025] 1.2 Experimental reagents

[0026] L-15 medium, trypsin, FITG labeled rabbit anti-mouse, fetal bovine serum, yeast powder, peptone, agar powder, tetracycline stock solution, Tween-20 (tween-20), bovine serum albumin BSA, M13 phage single chain DNA extraction kit, IPTG, X-gal, PEG-8000, TMB.

[0027] 1.3 Experimental working solution

[0028] 1×PBS, LB liquid medium, LB-Tet solid plate, top agar, IPTG / X-gal working solution, IPTG / X-gal plate, PEG-NaCl, TBS buffer, 0.1% TBST, 0.5% TBST, 4 %paraformaldehyde fixative, preparation of TBS-NaN3 solution, 3% BSA blocking solution, sodium iodide buffer, TE buffer, TMB working solution, tetracycline storage solution.

[0029] 2. Experimental method

[0030] 2.1 Culture of Escherichia co...

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Abstract

The invention belongs to the field of biomedicines and particularly relates to specific polypeptide targeting at human colon cancer cells and the application of the polypeptide. The polypeptide is anyone of the following components: (1) polypeptide with an amino acid sequence as: YPEIWPTDHKLM (SEQ ID No.1); (2) a polypeptide derivative which is formed by deleting, inserting or replacing one or more amino acids of polypeptide molecules of the polypeptide (1) and has biological functions same as those of the polypeptide molecules of the polypeptide (1). The polypeptide is capable of being specifically combined with colon cancer cells, has no specific action with normal enterocyte, and in addition is remarkable in action effect, and reliable scientific bases can be provided for clinical treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a specific polypeptide targeting human colon cancer cells and its application. Background technique [0002] Colon cancer is one of the most common digestive tract malignancies, and its incidence rate is second only to gastric cancer and esophageal cancer. The age of occurrence is generally over 50 years old, and there are more men than women, with a male to female ratio of 2:1. The etiology of colon cancer is still unclear, mainly environmental factors, genetic factors, precancerous diseases and so on. Domestic surveys show that the postoperative survival rate of early colon cancer is as high as 90-95%. However, in my country, more than 80% of patients have developed to the middle and late stages when they are diagnosed, and the early diagnosis rate is only 10-15%. The survival rate is only 5%. Therefore, early diagnosis and early treatment can significantly improve the ...

Claims

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Application Information

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IPC IPC(8): C07K7/08G01N33/574G01N33/68A61K47/42A61P35/00
CPCA61K47/42A61P35/00C07K7/08G01N33/57446G01N33/68
Inventor 赵琳魏敏杰于丽凤贾真卫倩
Owner 中国医科大学
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