Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A screening method and application of microorganisms containing MVA and/or MEP metabolic pathways

A screening method and derivative technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganism measurement/inspection, etc., can solve the problems of cumbersome operation, many intermediate links, low efficiency of screening amount and screening speed, etc., and achieve Obvious color development and good screening continuity

Active Publication Date: 2021-07-09
CABIO BIOTECH WUHAN CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former is highly subjective and has a large workload; the latter is cumbersome to operate, with many intermediate links, and the result is greatly affected by the indicator
The two are constrained by the workload, and both the amount of screening and the screening speed are obviously inefficient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A screening method and application of microorganisms containing MVA and/or MEP metabolic pathways
  • A screening method and application of microorganisms containing MVA and/or MEP metabolic pathways
  • A screening method and application of microorganisms containing MVA and/or MEP metabolic pathways

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Pour conventional PDA medium into a test tube to make a slant, inoculate B. trispora positive bacteria, culture at 26°C for 7 days, then add sterile water, mix and pour it into sterilized at 121°C for 40 minutes In an Erlenmeyer flask (containing glass beads) containing sterile water, break up the spores to obtain a spore suspension.

[0056] After diluting the above spore suspension 100 times, spread it on a plate containing the separation medium and culture until a single colony of B. trispora grows. Wherein, the separation medium includes conventional solid PDA medium and sodium deoxycholate with a concentration of 0.01 wt%.

[0057] The spores of B. trispora negative bacteria were inoculated into the seed medium, and then cultured at 26° C. and 200 rpm for 50 hours to obtain heterosexual bacterial seed liquid. The seed medium contains 1wt% glucose, 2wt% corn steep liquor dry powder, 0.5wt% yeast extract, 0.04wt% potassium dihydrogen phosphate, 0.005wt% magnesium su...

Embodiment 2

[0060] Pour conventional PDA medium into a test tube to make a slant, inoculate B. trispora negative bacteria, culture at 30°C for 5 days, then add sterile water, mix and pour into sterilized at 121°C for 40 minutes In an Erlenmeyer flask (containing glass beads) containing sterile water, break up the spores to obtain a spore suspension.

[0061] After diluting the above spore suspension 1000 times, spread it on a plate containing the separation medium and culture until a single colony of B. trispora grows. Wherein, the separation medium includes conventional solid PDA medium and derivatives of sodium deoxycholate at a concentration of 1 wt%.

[0062] The spores of B. trispora were inoculated into the seed medium, and then cultured at 30° C. and 240 rpm for 40 hours to obtain heterosexual seed liquid. Seed medium contains 3wt% glucose, 4wt% corn steep liquor dry powder, 1.5wt% yeast extract, 0.1wt% potassium dihydrogen phosphate, 0.015wt% magnesium sulfate, 0.04wt% sodium glu...

Embodiment 3

[0066] Pour conventional PDA medium into a test tube to make a slant, inoculate B. trispora positive bacteria, culture at 28°C for 6 days, then add sterile water, mix and pour into sterilized at 121°C for 40 minutes In an Erlenmeyer flask (containing glass beads) containing sterile water, break up the spores to obtain a spore suspension.

[0067] After diluting the above spore suspension 1000 times, spread it on a plate containing the separation medium and culture until a single colony of B. trispora grows. Wherein, the separation medium includes conventional solid PDA medium and sodium deoxycholate with a concentration of 0.01 wt%.

[0068] The spores of B. trispora negative bacteria were inoculated into the seed medium, and then cultured at 28° C. and 220 rpm for 48 hours to obtain heterosexual bacterial seed liquid. The seed medium contains 2wt% glucose, 3wt% corn steep liquor dry powder, 1wt% yeast extract, 0.07wt% potassium dihydrogen phosphate, 0.01wt% magnesium sulfate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a screening method and application of microorganisms containing MVA and / or MEP metabolic pathways, belonging to the field of biosynthesis and fermentation engineering. The above-mentioned screening method comprises the following steps: inoculate the spore suspension of microorganisms into a culture container for cultivation to obtain single colonies of microorganisms, cultivate them in a closed environment filled with ethylene mixed gas for 24-48 hours, and screen out orange or red colonies. Microorganisms include genetically engineered bacteria introduced with exogenous MVA and / or MEP metabolic pathways, and any of the microorganisms naturally containing the above metabolic pathways. The mixed gas includes ethylene and air. This method is low in cost, easy to operate, and has obvious color development, and has the advantages of high screening throughput and short cycle. The above screening method is used to optimize the B. trispora strains producing β-carotene or lycopene, which can make the strains develop color in a short time, which is beneficial to the strains with high-throughput carotenoid metabolic flux. Identification, efficient and convenient.

Description

technical field [0001] The invention relates to the fields of biosynthesis and fermentation engineering, and in particular to a screening method and application of microorganisms containing MVA metabolic pathway and / or MEP metabolic pathway. Background technique [0002] Carotenoids are natural pigments of great economic value, especially β-carotene and lycopene, which have been used as functional raw materials in food and health care due to their high antioxidant properties and the characteristics of eliminating free radicals in the human body. widely used in products. The known strong antioxidant properties of lycopene can also be used to prevent cancer, further highlighting its medicinal value and economic prospects. [0003] The B. trispora fermentation method is currently the only method that has been used in large-scale and industrialized biological fermentation to produce high-quality carotenoids. While ensuring the natural source of carotenoids, this method can als...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/645
CPCC12Q1/04
Inventor 汪志明李翔宇刘洋余超陆姝欢
Owner CABIO BIOTECH WUHAN CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com