Sialic acid electrochemical test paper as well as preparation method and detection method thereof
A sialic acid and electrochemical technology, which is applied in the field of sialic acid electrochemical test paper and its preparation and detection, can solve the problems of photochemical colorimetric method, such as large blood collection, high price, and long time required for analysis results
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Embodiment 1
[0090] This embodiment provides a preparation example of a sialic acid test paper to illustrate the sialic acid test paper and preparation method of the present invention.
[0091] Firstly, the working electrode 2-1, the reference electrode 2-2 and the short-circuit electrode 2-3 are made on the 0.25mm thick PET plastic substrate, ie, the insulating substrate 1, using screen printing technology, using conductive carbon ink purchased by Acheson Production, spread the conductive ink on the PET plastic substrate with a thin layer of evenly dispersed ink by screen printing, then put it in an oven at 80°C for 60 minutes;
[0092] Secondly, using screen printing technology to print the insulating varnish on the substrate of the printed electrode system as the electrical insulating isolation layer 3. The insulating varnish was purchased by Acheson, and the insulating varnish was spread on the PET plastic substrate by screen printing with evenly dispersed ink After the thin layer, put it i...
Embodiment 2
[0105] This example illustrates the linear relationship between the concentration of sialic acid and the current, and the method of constructing a calibration curve using the sialic acid test paper prepared in Example 1.
[0106] Collect fresh blood with a packed volume ratio of 45%, prepare blood samples with sialic acid concentrations of 0.5, 1.0, 1.5, 3.0, 5.0, and 7.0 mmol / L, and place the sialic acid test paper prepared in Example 1 on a multi-parameter analyzer Apply 300mV working voltage to both ends of working electrode 2-1 and reference electrode 2-2; take blood with a pipette to contact sampling port 7, blood will automatically draw about 2μL of sample through siphon guide groove 6, and it will be hydrolyzed by pretreatment layer 5. Part of the combined sialic acid reaches the reaction zone and reacts with the reagents in the enzyme reaction membrane 4 to form a current. The current corresponding to the concentration of sialic acid can be quickly detected on the multi-pa...
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