Sialic acid electrochemical test paper as well as preparation method and detection method thereof

Abstract

The invention discloses a sialic acid electrochemical test paper as well as a preparation method and a detection method thereof. The sialic acid electrochemical test paper comprises an insulating substrate, a conductive electrode, an insulated isolation layer, a reaction layer, a pretreatment layer, a siphon sample guide groove and an adhesion spacer; the reaction layer is an enzyme reaction membrane; the enzyme reaction membrane covers the surfaces of a reference electrode and a working electrode of a reaction region and is arranged at the reaction region end of the insulated isolation layer;the pretreatment layer is arranged at the front end of the reaction layer; the reaction layer and the pretreatment layer are arranged in the siphon sample guide groove; the enzyme reaction membrane comprises an enzyme which is reacted with sialic acid and an electron transfer mediator; and slurry of the pretreatment layer contains a sialic acid hydrolytic agent. For the sialic acid electrochemical test paper provided by the invention, by using a method of detecting the sialic acid with a direct electrochemical biosensor principle, by arranging a sample pretreatment structure and combining enzyme in an enzyme layer, the sialic acid in blood is rapidly and thoroughly decomposed into free sialic acid, so that the test sensitivity of the sialic acid is improved, and a method of accurately, rapidly and simply detecting the sialic acid with low cost is realized.

Description

Technical field [0001] The invention relates to the field of electrochemical test paper and electrochemical detection, in particular to a sialic acid electrochemical test paper and a preparation and detection method thereof. Background technique [0002] Sialic acid (SA) is a general term for a class of carboxylated monosaccharide acylated derivatives containing 9 carbon atoms. The main sialic acids in the human body are N-acetylneuraminic acid and N-glycolylneuraminic acid Two are mostly produced by glucose metabolism. Serum sialic acid is derived from the surface of cells and exists in two forms: sugar-bound sialic acid and lipid-bound sialic acid. [0003] The level of sialic acid expression in various tissues during the developmental stage of the body is higher, while it is low in adult tissues. If the level of sialylation in adult tissues increases, it indicates the possibility of pathological changes. In the 1970s, foreign scholars through animal experiments and human tumor...

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Problems solved by technology

Among these methods, the detection process of high-performance liquid chromatography and chemiluminescence method is cumbersome, and it takes a long time to obtain the analysis results; the photochemical colorimetric method takes a large amount of blood, and requires a special biochemical analyzer, which is expensive

There is no report on the electrochemical method and product for rapid detection of sialic acid that can be used at home or at the bedside

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