Application of alpha-oxoglutarate to improving heat production capability of fat animals and reducing body fat content
A technology of ketoglutaric acid and heat generating capacity, which is applied in the field of animal metabolism to achieve the effect of being safe and easy to obtain, without toxic side effects, and with a small amount of addition.
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Embodiment 1
[0033] Example 1 α-ketoglutaric acid can improve the oxygen consumption and heat production capacity of mice fed with high-fat diet
[0034] 1. In order to study the effect of α-ketoglutarate (AKG) on the oxygen consumption and heat production capacity of mice fed with high-fat diet, 10-week-old female C57BL / 6 mice were used as the research object.
[0035] 2. Experimental method
[0036] (1) Animal feeding conditions: 1 animal / box, single breeding; feeding temperature and humidity: 20-26°C, 40%-70%; 12 h: 12 h day and night intermittent lighting; the conditions of the breeding room are always kept stable to ensure the test reliability of the results. Free access to food and water, feed and drinking water were provided by the Guangdong Provincial Medical Experimental Animal Center; Quarantine: Pre-feeding of purchased SPF grade C57BL / 6 J mice for 7 days. Animals were checked once a day during this period.
[0037] (2) Animal grouping and treatment: Twenty 10-week-old female...
Embodiment 2
[0045] Example 2 Adding 2% α-ketoglutarate to drinking water to reduce body fat content in mice fed a high-fat diet
[0046] 1. In order to study the effect of α-ketoglutarate on the body fat content of high-fat fed mice, 10-week-old female C57BL / 6 mice were used as the research object.
[0047] 2. Experimental method
[0048] (1) Animal feeding method: same as in Example 1.
[0049] (2) Grouping and treatment of animals: the same as in Example 1.
[0050] (3) Sampling and detection: Body composition and body imaging tests were performed at the eighth week of the experiment.
[0051] 3. The result is as follows figure 2 Shown:
[0052] (1) The results are attached figure 2 As shown in A and 2B, supplementation of drinking water with 2% α-ketoglutarate significantly reduced the total fat content of mice fed a high-fat diet compared with the high-fat group.
[0053] (2) At the 20th week of the experiment, the abdominal fat and subcutaneous fat of the mice were sampled an...
Embodiment 3
[0055] Example 3 Adding 2% α-ketoglutarate to drinking water can improve the heat production capacity of brown fat in mice fed a high-fat diet
[0056] 1. In order to study the effect of α-ketoglutarate on the heat production ability of brown fat in mice fed with high fat, the experiment took 10-week-old female C57BL / 6 mice as the research object.
[0057] 2. Experimental method
[0058] (1) Animal feeding method: same as in Example 1.
[0059] (2) Grouping and treatment of animals: the same as in Example 1.
[0060] (3) Sampling and detection: In order to further study the effect of α-ketoglutarate on the expression of thermogenic genes in brown fat of mice, we collected brown adipose tissue of mice and detected the related thermogenic genes in brown fat of mice protein expression and mRNA expression.
[0061] 3. The result is as follows image 3 Shown:
[0062] (1) if image 3 As shown in A and 3B, compared with the high-fat group, drinking water supplemented with 2% α...
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