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Liquid crystal-based detection kit for visually detecting tumor marker

A tumor marker and detection kit technology, which is applied in the field of detection kits for visual detection of tumor markers, can solve the problems of weak anti-interference ability and low detection sensitivity, avoid non-specific adsorption, solve the problem of low sensitivity, Effect of improving detection sensitivity

Inactive Publication Date: 2018-09-14
YUNNAN UNIV
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Problems solved by technology

[0003] The invention provides a liquid crystal-based detection kit for the visual detection of tumor markers. The kit is capable of simple, rapid and highly sensitive visual detection of tumor markers. It is a liquid crystal immunoassay method to overcome the detection sensitivity of the prior art Low, weak anti-interference ability and other issues

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  • Liquid crystal-based detection kit for visually detecting tumor marker
  • Liquid crystal-based detection kit for visually detecting tumor marker
  • Liquid crystal-based detection kit for visually detecting tumor marker

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Embodiment 1

[0061] The detection of embodiment 1 tumor marker-CEA

[0062] The method of the present invention will be described in detail below by taking CEA as an example.

[0063] 1. Method

[0064] 1. Preparation of immunomagnetic spheres

[0065] Take 5 μL of superparamagnetic microspheres (50 mg / mL, 500 nm in diameter) containing carboxyl functional groups, wash them three times with PBS (0.1 mol / L, pH 6.0), and then disperse them in a solution containing 0.1 mol / L NHS and 0.1 mol / L EDC In a PBS buffer (pH 6.0, 0.1 mol / L) solution, activate for 40 minutes on a constant temperature shaker at 130 rpm at 37°C. Wash three times with PBS buffer solution (pH 7.2, 0.1mol / L), then disperse it into 600μL PBS buffer solution (0.1mol / L, pH 7.2), add 20μL 0.64mg / mL CEA capture antibody (1# Ab), placed on a constant temperature shaker at 37°C for 12 hours, then washed three times with pH 7.20.1mol / L PBS buffer solution to remove excess antibody, and dispersed into 600μL PBS buffer containing ...

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Abstract

The invention relates to a detection kit for detecting a tumor marker, which belongs to the detection field of analysis. An immune magnetic ball which is covalently coupled with a tumor marker antibody is used as a solid vector of the immune reaction, an immune magnetic separation technology is used for separating and gathering the target tumor markers, a catalytic signal of a marker enzyme is converted into metal nano particles deposited on the surface of a liquid crystal sensitive membrane to disturb liquid crystal molecules according to an enzymatic metallization signal conversion and amplification strategy, so that the visual detection of the target tumor marker can be realized. According to the method, the immune magnetic separation technology is combined with the enzymatic metallization and a liquid crystal biosensor to establish a liquid crystal immunoassay based on the immune magnetic separation, so that the problem that when the liquid biosensor is applied to the immunoassay,the sensitivity is low, and the interference resistance is low can be solved. The method has the characteristics of high sensitivity, simplicity in operation, no need of complicated instruments, visualized detection result and the like and can be directly used for detecting the tumor marker in a complicated sample.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a liquid crystal-based detection kit for visual detection of tumor markers. Background technique [0002] Liquid crystal biosensor is a kind of analysis and detection method established by using liquid crystal molecules as signal conversion elements. Since the ordered alignment of liquid crystal molecules is very sensitive to the physical and chemical properties of the basement membrane surface, it is usually possible to use the changes in the surface morphology and properties of the sensitive membrane caused by the specific recognition of biomolecules on the surface of the sensitive membrane. Detection of target biomolecules. However, the non-specific adsorption of the complex matrix on the surface of the sensitive membrane will also affect the ordered arrangement of the liquid crystal molecules, thus limiting its application in the actual complex sam...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57484
Inventor 周川华字琴江王瑾赵文颖曹秋娥
Owner YUNNAN UNIV
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