Phase chromatography tandem mass spectrum method for NNN optical isomer separation and detection in tobacco

Abstract

The invention relates to a phase chromatography tandem mass spectrum method for N-nitrosonornicotine (NNN) optical isomer separation and detection in tobacco, and belongs to the technical field of tobacco chemistry analysis. A methanol solution containing 0.5% of acetic acid is used for extracting (S)-NNN and (R)-NNN in a tobacco sample, and after the extracting solution is filtered, UPC2-MS / MS isused for analyzing and quantifying the concentration of (S)-NNN and (R)-NNN in the extracting solution. The sample treating process is simple and quick, and batched strict work analysis is convenient. When the phase chromatography tandem mass spectrum is used for detection, the used main moving phase is supercutical fluid CO2, a small amount of methyl alcohol is used as a cosolvent, and the environmentally friendly advantage is achieved. According to the method, the detection limit of (S)-NNN is 0.21 ng / mL, the quantitation limit is 0.69 ng / mL, the detection limit of (R)-NNN is 0.34 ng / mL, and the quantitation limit is 1.12 ng / mL. The method is high in sensitivity and good in repeatability and is applied to the separation and measurement of the N-nitrosonornicotine optical isomer in the tobacco sample.

Description

technical field [0001] The present invention relates to a kind of tobacco N - A convergence chromatography tandem mass spectrometry method for the separation and determination of nitrosonornicotine (NNN) optical isomers, which belongs to the field of tobacco chemical analysis. Background technique [0002] N -Nitrosonornicotine (NNN) is produced by the nitrosation of the tobacco alkaloid nornicotine during tobacco conditioning and processing. important cause. In its molecular structure, the carbon atom at the 2-position on the tetrahydropyrrole ring has chirality, so NNN has two enantiomers, namely ( S )-NNN and ( R )-NNN. Toxicology research certificate ( S )-NNN is more carcinogenic than ( R )-NNN. Therefore, the separation and determination of NNN enantiomers in tobacco can provide potential references for research on smoking and health, and also provide potential new ideas for research on reducing the harm of cigarettes. [0003] At present, there are few litera...

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Problems solved by technology

And about ( S )-NNN and ( R )-NNN separation by convergence chromatography or convergence chromatography tandem mass spectrometry has not been reported

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