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Brachybacterium nesterenkovii-containing microbial agent

A technology of Brevibacterium nissleri and bacterial agents, applied in the field of bacterial agents containing Brevibacterium nierlii NY-1, which can solve the problems of low treatment efficiency of volatile organic chlorine, uneven loading, and packing collapse, etc. Lightweight, shortened start-up period, and small pressure loss

Inactive Publication Date: 2018-09-14
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problems of low volatile organic chlorine treatment efficiency, uneven loading of microorganisms on the surface of the mixed carrier, slow start-up, and collapse of fillers after long-term use in the prior art, the present invention provides a strain of Brachybacterium nesterenkovii NY- 1, and apply the strain to remove waste gas containing organic chlorine

Method used

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Embodiment 1

[0037] Embodiment 1: A kind of bacterial agent and application for purifying waste gas containing organic chlorine

[0038] The present invention also provides a bacterial agent prepared by Brevibacterium nierii NY-1, which can be used to purify waste gas containing organic chlorine and solve the pollution problem of volatile organic chlorine produced in processes such as pesticide wastewater treatment. The preparation method of agent is as follows:

[0039] (1) Fermentation broth preparation method

[0040] Brevibacterium Niezii NY-1 was cultured on a slant and then inserted into the liquid fermentation medium, and the inoculation amount was 10% to carry out step-by-step amplified culture. The fermentation culture conditions were: 36°C, ventilation rate 10-100L / min, dissolved oxygen > 2.0 mg / L, tank pressure 0.03-0.10Mpa, culture time 48h;

[0041] The medium composition of slant culture is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2.0g, NH 4 Cl 0.5g, MgCl ...

Embodiment 2

[0048] Embodiment 2: A kind of bacterial agent and application for purifying waste gas containing organic chlorine

[0049] The present invention also provides a bacterial agent prepared by Brevibacterium nierii NY-1, which can be used to purify waste gas containing organic chlorine and solve the pollution problem of volatile organic chlorine produced in processes such as pesticide wastewater treatment. The preparation method of agent is as follows:

[0050] (1) Fermentation broth preparation method

[0051] After being cultured on a slant, Brevibacterium Niezii NY-1 was inserted into a liquid fermentation medium with a 25% inoculum size for step-by-step amplification culture. The fermentation culture conditions were: at 36°C, ventilation rate 10-100L / min, dissolved oxygen >2.0mg / L, tank pressure 0.03-0.10Mpa, culture time 72h;

[0052] The culture medium of described slant consists of: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2.0g, NH 4 Cl 0.5g, MgCl 2 ·6H ...

Embodiment 3

[0059] Embodiment 3: A kind of bacterial agent and application for purifying waste gas containing organic chlorine

[0060] The present invention also provides a bacterial agent prepared by Brevibacterium nierii NY-1, which can be used to purify waste gas containing organic chlorine and solve the pollution problem of volatile organic chlorine produced in processes such as pesticide wastewater treatment. The preparation method of agent is as follows:

[0061] (1) Fermentation broth preparation method

[0062] Brevibacterium nirzia NY-1 was cultured on a slant and then inserted into the liquid fermentation medium. The inoculum size was 15% and the culture was scaled up step by step. The fermentation culture conditions were: at 36°C, ventilation rate 10-100L / min, dissolved oxygen 2.0mg / L, tank pressure 0.03-0.10Mpa, culture time 24h;

[0063] The medium composition of slant culture is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 32.0g, NH 4 Cl 0.5g, MgCl 2 ·6H 2 O ...

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Abstract

The invention belongs to the field of environmental treatment and particularly relates to a brachybacterium nesterenkovii-containing microbial agent. Specifically, applied brachybacterium nesterenkovii is brachybacterium nesterenkovii NY-1 with a preservation number of CGMCC No. 13747. The brachybacterium nesterenkovii-containing microbial agent is high in organochlorine degrading efficiency of upto 90-96%, short in adaptive period of dominant bacteria, and capable of rapidly starting degrading reaction within one week, and when applied to a biological reactor for purifying organochlorine-containing waste gas, can shorten the starting period.

Description

Technical field: [0001] The invention belongs to the field of environmental management, in particular to a bacterial agent containing Brachybacterium nesterenkovii NY-1. Background technique: [0002] Organochlorine pesticides are organic compounds containing organochlorine elements in the components used to prevent and control plant diseases and insect pests, and are recognized as a very important class of persistent organic pollutants. During the production of organochlorine pesticides and the treatment of pesticide wastewater, volatile pesticide products or intermediates volatilize from the liquid phase and escape into the air, forming air pollution. Volatile pesticides and intermediates include: trichlorfon, dichlorvos, phosphorus trichloride, DDT, chloral urea, sodium trichloroacetate, HHC, aldrin, chlordane, endrin, seven Chlorine, toxaphene, hexachlorobenzene, mirex, chloral, etc. Most of these substances have a pungent smell, and some substances, such as chloral, ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/85B01D53/70C12R1/01
CPCB01D53/685B01D53/70B01D53/85B01D2251/95B01D2257/2064C12N1/20Y02A50/20
Inventor 刘俊新杨唐李琳李文凯
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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