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Method for extracting polysaccharides from ganoderma lucidum mycelia

A technology of Ganoderma lucidum mycelium and Ganoderma lucidum polysaccharide, which is applied in the field of microorganisms, can solve the problems of difficult dissolution, difficulty of Ganoderma lucidum mycelium, and destruction of polysaccharide activity, etc., and achieves the effects of easy breakage, short cultivation period and simple cultivation method

Active Publication Date: 2018-09-14
HUNAN YUJIA COSMETICS MFG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, on the one hand, it is not easy to obtain Ganoderma lucidum mycelium by liquid fermentation; on the other hand, mycelium ganoderma acid exists in the cell wall of Ganoderma lucidum mycelium, and it is not easy to dissolve during the extraction process, and the method of fully breaking the wall is always unavoidable. activity of polysaccharides

Method used

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  • Method for extracting polysaccharides from ganoderma lucidum mycelia
  • Method for extracting polysaccharides from ganoderma lucidum mycelia
  • Method for extracting polysaccharides from ganoderma lucidum mycelia

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Embodiment 1

[0043] Determination of total polysaccharide content in embodiment 1 Ganoderma lucidum mycelium

[0044] 1. The Ganoderma lucidum mycelia fermentation broth is centrifuged to obtain the Ganoderma lucidum mycelium, and the Ganoderma lucidum mycelium is dried in an oven at 60°C;

[0045] 2. Take 10g of Ganoderma lucidum mycelium, add 100ml of water in sequence according to the ratio of 1:10, mix well and form a mixture of Ganoderma lucidum mycelia;

[0046] 3. Place the mixture in hot water at 80°C, stir, and ultrasonically treat for 20 minutes;

[0047] 4. Centrifuge after ultrasonic treatment, take out the supernatant, and detect the polysaccharide content of Ganoderma lucidum according to the phenol-sulfuric acid method;

[0048] 5. The precipitate after centrifugation was repeated 5 times according to steps 2 to 4, and the polysaccharide was extracted 6 times in total;

[0049] 6. Calculate the total content of polysaccharides in the mycelium of Ganoderma lucidum according...

Embodiment 2

[0061] 1) Ganoderma lucidum mycelium is obtained by centrifuging the fermentation liquid of ganoderma lucidum mycelia, and the mycelia of ganoderma lucidum is dried in an oven at 60° C. for later use;

[0062] 2) Take 100g of Ganoderma lucidum mycelium, add 3000ml of water, use a high-speed homogenizer to homogenize at a speed of 5000rpm for 5 minutes, and mix evenly to form a mixed liquid of Ganoderma lucidum mycelia;

[0063] 3) The pressure of the high-pressure homogenizer is set to 500 bar, and the number of cycles is 1 time;

[0064] 4) Centrifuge at high speed to obtain supernatant.

Embodiment 3

[0066] 1) Ganoderma lucidum mycelium is obtained by centrifuging the fermentation liquid of ganoderma lucidum mycelia, and the mycelia of ganoderma lucidum is dried in an oven at 60° C. for later use;

[0067] 2) Take 100g of Ganoderma lucidum mycelium, add 3000ml of water, use a high-speed homogenizer to homogenize at a speed of 5000rpm for 5 minutes, and mix evenly to form a mixed liquid of Ganoderma lucidum mycelia;

[0068] 3) The pressure of the high-pressure homogenizer is set to 500 bar, and the number of cycles is 2 times;

[0069] 4) Centrifuge at high speed to obtain supernatant.

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Abstract

The invention relates to the technical field of microbes and particularly relates to a method for extracting polysaccharides from ganoderma lucidum mycelia. The method comprises the steps of mixing dried ganoderma lucidum mycelia with water, firstly, carrying out uniform mixing by a high-speed homogenizer, then, carrying out homogeneous dispersion for 1 to 5 times by high pressure of 500bar to 1500bar, and then, carrying out centrifugation, thereby obtaining polysaccharide-containing supernatant. Compared with the traditional water extraction method, the method has the advantages that the yield of the ganoderma lucidum polysaccharides can be increased, the time of extraction of the ganoderma lucidum polysaccharides is shortened, and the oxidation resistance and anti-inflammation effect ofthe ganoderma lucidum polysaccharides are improved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for extracting polysaccharides from ganoderma lucidum mycelia. Background technique [0002] Ganoderma lucidum (Curtis: Fr.) P.Karst. is a fungus of the genus Ganoderma in the family Polyporaceae of Basidiomycetes, and it has been a valuable medicinal fungus in my country since ancient times. It can treat a variety of diseases, nourish and strengthen, strengthen the body and strengthen the body, and is a valuable traditional Chinese medicine that is conducive to maintaining or restoring the body's homeostasis. Modern medical research has found that the main physiologically active ingredients in Ganoderma lucidum are Ganoderma lucidum polysaccharides and Ganoderma acid, which have anti-tumor, lower blood cholesterol levels, and anti-inflammatory effects. In recent years, a series of studies have found that Ganoderma lucidum polysaccharides not only have immunomodu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P39/06A61P29/00A61K8/73A61Q19/08A61Q19/00
CPCA61K8/73A61K31/715A61K2800/85A61P29/00A61P39/06A61Q19/00A61Q19/08C08B37/00C08B37/0003
Inventor 戴跃锋康文术何广文颜少慰
Owner HUNAN YUJIA COSMETICS MFG CO LTD
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