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Method for Establishing Fingerprint of Danggui Sini Decoction Composition

A technology for fingerprinting and establishing methods, applied in the field of traditional Chinese medicine analysis, can solve the problems of single quality control, many components, and few indicators, and achieve the effects of controlling the quality of preparations, objective conclusions, and convenient operation.

Active Publication Date: 2020-08-07
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome the traditional Chinese medicine compound preparations in the prior art, which basically only have one or two index components in content control, single quality control, few indexes, and it is difficult to fully reflect that there are many flavors and many components. , the defects of the quality status of the Danggui Sini Decoction composition with many targets, thereby providing a method for establishing the fingerprint of the Danggui Sini Decoction composition

Method used

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  • Method for Establishing Fingerprint of Danggui Sini Decoction Composition
  • Method for Establishing Fingerprint of Danggui Sini Decoction Composition
  • Method for Establishing Fingerprint of Danggui Sini Decoction Composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1. The preparation of Angelica Sini Decoction composition

[0041] Take Angelica 375g; Guizhi 375g; Paeoniae Alba 375g; Asarum 125g; Radix Glycyrrhiza 250g; Filtrate, concentrate the filtrate to a clear paste with a relative density of 1.20-1.25 (60° C.), add an appropriate amount of maltodextrin, mix well, dry, and granulate to make 1000 g to obtain the composition of Angelica Sini Decoction.

Embodiment 2

[0042] Example 2. Establishing the Fingerprint of Danggui Sini Decoction

[0043] Instruments and reagents

[0044] Chromatography instrument: American Agilent 1100 series (G1322A online vacuum degasser, G1311A quaternary gradient pump, G1313A autosampler, G1316A column thermostat, G1315B diode array detector);

[0045] Chromatographic column: Kromasil 100-5-C18 4.6×250mm 5μm;

[0046] Reagents: methanol, Tianjin Damao Chemical Reagent Factory, chromatographically pure, batch number: 20160310; acetonitrile, Tianjin Biaoshiqi Technology Development Co., Ltd., chromatographically pure, batch number: AC-12060107; phosphoric acid, Tianjin Tongguang Fine Chemical Company, analysis Pure, batch number: 20150712; water, Millipore ultrapure water;

[0047] Danggui Sini Decoction Composition Medicinal Materials: Three batches of Danggui Sini Decoction composition 160501, 160502, 160504 were provided by China Resources Sanjiu Group for trial production according to the steps of Example...

Embodiment 3

[0062] Embodiment 3. Methodological investigation of Danggui Sini Decoction fingerprint

[0063] Precision inspection

[0064] Take 1 part of Danggui Sini Decoction composition (160501), prepare the test solution according to the method in the text, inject the sample continuously for 6 times, and determine the fingerprint spectrum. image 3 , using fingerprint software to evaluate the similarity, the calculation results are shown in Table 2, and the retention time and relative standard deviation of the peak area of ​​some chromatographic peaks are calculated, as shown in Table 3. The results show that the precision of the instrument is good.

[0065] Table 2 The similarity calculation results of the precision inspection of the fingerprints of Danggui Sini granules

[0066]

[0067] Table 3 precision examines part of the chromatographic peak retention time and peak area relative standard deviation

[0068]

[0069]

[0070]

[0071]

[0072] Stability study

...

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Abstract

The invention discloses an establishing method of a danggui sini decoction fingerprint atlas and the fingerprint atlas, wherein the method and the fingerprint atlas belong to the field of traditionalChinese medicine analysis. According to the method of the invention, the fingerprint atlas is established according to high-efficiency liquid chromatography; a chromatographic column is an octadecylsilane chemically bonded silica chromatographic column; the column temperature is 25-35 DEG C; the flow velocity is 0.9-1.1ml / min; the detecting wavelength is 220nm; acetonitrile A-0.1% phosphoric acidB is used as a mobile phase; and gradient elution is performed according to a sequence which is shown in the description. The fingerprint atlas according to the invention comprehensively reflect quality information of the danggui sini decoction, thereby realizing a purpose of more comprehensively and effectively controlling the quality of a danggui sini decoction composition product.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a quality standard detection method of traditional Chinese medicine compound granules, in particular to a method for establishing fingerprints of Danggui Sini Decoction composition. Background technique [0002] Traditional Chinese medicine decoction is the mainstream of clinical medicine, but because of decoction and inconvenient to carry, the quality of the decoction prepared by decoction often varies greatly depending on the person and the decoction utensils. The use of modern science and technology to develop traditional Chinese medicine preparations that are easy to carry and easy to take is not only the inheritance of traditional Chinese medicine, but also a better promotion of classic famous prescriptions. Clinical application. The preparation process of granules is relatively simple, easy to shape, easy to take and carry, and the form of taking is most ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 林丽娜刘英陈周全刘志刚谭沛马鹏岗高雅高云佳屠鹏飞
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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