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Method for extracting polyphenol from sea-buckthorn leaves with supercritical CO2 fluid extraction technology

A seabuckthorn leaf and supercritical technology, which is applied in pharmaceutical formulas, medical preparations containing active ingredients, anti-toxins, etc., can solve problems that have not been reported, and achieve the effect of improving extraction efficiency

Active Publication Date: 2018-08-24
内蒙古宇航人高技术产业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although using supercritical CO 2 Fluid extraction technology has been reported to extract some plant polysaccharides (such as shiitake mushrooms, Poria cocos, etc.), but supercritical CO 2 The study of extracting polyphenols from Seabuckthorn leaves by fluid extraction technology has not been reported yet

Method used

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  • Method for extracting polyphenol from sea-buckthorn leaves with supercritical CO2 fluid extraction technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1. Preparation of Seabuckthorn Leaf Polyphenol Extract

[0034] Mix 100g of 20-mesh seabuckthorn leaf powder with 100ml of 60% ethanol solution (pH 4), soak overnight, put the soaked material in a 300ml extraction kettle, fix the filled extraction kettle in the heating box, and connect the extraction kettle Both ends of the interface, close the intake switch, outlet valve and fine-tuning valve, close the door of the heating box; open the supercritical CO 2For the power supply of the fluid extraction equipment, set the temperature of the extraction kettle to 40°C and the temperature of the fine-tuning valve to 90°C. When the temperature reaches the set value, turn on the inlet switch, adjust the pressure of the extraction kettle to 30MPa, and keep it under this pressure for 10 minutes; open the outlet valve , use the fine-tuning valve to control the gas flow in the recovery bottle, so that the flow meter at the end shows that the flow rate is 2L / min, balance the ...

Embodiment 2

[0036] Effects of ethanol immersion

[0037] The comparison test was carried out by soaking and non-immersing methods, and the yield and polyphenol content were compared for three times, and the results obtained were as follows:

[0038] (yield / polyphenol content)

[0039] It can be seen from the above table that, no matter in terms of the yield of the final product or the content of extracted polyphenols, the method of soaking first and then extracting has made significant progress.

Embodiment 3

[0041] Effect of pH of ethanol solution

[0042] The inventors used a gradient method to carry out comparative experiments, using the pH values ​​of the original ethanol solution (about 6.9), 6.5, 6.0, 5.5, 5.0, 4.5, 4.0, 3.5, 3.0, 2.5, 2.0, and 1.5. The results obtained are as follows:

[0043]

[0044] From the experimental results, it can be seen that the yield and polyphenol content were greatly improved when the pH gradient of the ethanol solution decreased at first, and basically showed a stable state when the pH was between 3-4, and when the pH decreased again, gradually When entering the strong acid zone, the yield and polyphenol content show a state of rapid decline. The inventor speculates that the reaction of too strong acidity may cause the destruction of the raw material structure, etc., and ultimately affect the product yield.

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Abstract

The invention discloses a preparation method of a sea-buckthorn polyphenol extract. Specifically, a raw material, namely, sea-buckthorn leaves, soaked in an acidic ethanol liquor is treated in a CO2 supercritical extraction manner, and the polyphenol extract with total antioxidant capacity and DPPH free radical scavenging rate IC50 higher than that of the prior art can be obtained.

Description

technical field [0001] The present invention relates to a method for extracting chemical components, in particular to a method for extracting polyphenols from seabuckthorn leaves, especially to a supercritical CO 2 Method for fluid extraction of polyphenols from seabuckthorn leaves. Background technique [0002] Seabuckthorn (Hippophaerhamnoides L.) is a shrub or small tree belonging to the genus Solanum in the family Elaeagnaceae. Seabuckthorn is widely planted in China. Its roots, stems, leaves, flowers and fruits contain more than 200 kinds of nutrients. Seabuckthorn leaves, as waste, are rich in polyphenols, which are the main active ingredients of seabuckthorn leaves, and have good effects in preventing and treating hyperglycemia, hyperlipidemia, cancer, and cell aging. [0003] In the past, researchers used water extraction, microwave, and ultrasonic methods to extract polyphenols from seabuckthorn leaves. These traditional extraction methods are often time-consuming...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P3/10A61P3/06A61P35/00A61P39/06A61K127/00
CPCA61K36/185A61K2236/333A61K2236/37A61K2236/51A61P3/06A61P3/10A61P35/00A61P39/06
Inventor 邢国良王捷吕兆林姚玉军王尚义闻茂马素清柳梅任璇崔彦民
Owner 内蒙古宇航人高技术产业有限责任公司
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