A kind of serum-free medium for cultivating adherent cells and application thereof

Abstract

The invention provides a serum-free culture medium for adherent cell culture and application of the serum-free culture medium, and belongs to the field of cell culture media. High-glucose DMEM (dulbecco's modified eagle media) and serum-reduced culture media are used as basic culture media, and the basic culture media contain serum substitutes, compound amino acid, penicillin and streptomycin. Theserum-free culture medium and the application have the advantages that the serum-free culture medium is free of cell culture media with animal-derived substances and accordingly is independent from animal serum; the serum-free culture medium comprises definite components, an alternative scheme for serum culture media is provided, and accordingly the serum-free culture medium is favorable for market promotion; the serum-free culture medium can be applied to the adherent cell culture; self-renewal of tumor cells and normal cell lines which are subjected to adherent culture can be maintained bythe serum-free culture medium, and long-time culture and passage can be implemented without increase of cell apoptosis proportions.

Description

technical field [0001] The invention belongs to the field of cell culture medium, and in particular relates to a serum-free culture medium for culturing adherent cells and an application thereof. Background technique [0002] Cell culture is the most commonly used basic technique in cell biology and tumor-related research. When cells are cultured in vitro, the medium provides the necessary external environment for cell growth, division, and proliferation, including buffers that simulate physiological acid-base concentrations, substances required for basic metabolism, and some nutritional factors. According to cell characteristics, cell culture methods are usually divided into suspension culture and adherent culture. Except for stem cells, lymphocytes and other blood system cells commonly used in suspension culture, most cells in the body, including normal cells and tumor cells, rely on adherence. wall culture. When culturing adherent cells, in addition to providing the cul...

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Problems solved by technology

And CN102352343A and CN104830765A disclosed culture medium scheme and fetal bovine serum substitute all are used for lymphocyte culture, and lymphocyte adopts suspension culture method, can't be used for the tumor cell of adherent culture

Therefore, in the above medium scheme, the tumor cells cannot adhere to the wall normally, nor can they proliferate normally. After a certain period of culture, the apoptosis rate increases significantly until all of them die, which affects the normal progress of experimental research.

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