Preparation of polyacrylonitrile modified membrane and method using polyacrylonitrile modified membrane for immobilizing enzyme
A polyacrylonitrile and modified technology, which is applied in the direction of being fixed on/in an organic carrier, can solve the problems of unsupported microorganisms, low gel film strength, low microorganism adhesion, etc., and achieves the effect of excellent performance.
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Embodiment 1
[0058] (1) Preparation of polyacrylonitrile hollow membrane
[0059] Prepare polyacrylonitrile hollow membrane by phase inversion method: add 10g polyacrylonitrile powder and 5mL porogen polyethylene glycol into 55mL N,N-dimethylformamide, mix well, seal and react at 65°C for 48h, and form brownish yellow Take out the viscous liquid, cool at room temperature for 12 hours to remove air bubbles; spread the reaction solution in a glass plate groove with a depth of 0.1 mm at room temperature, evaporate at room temperature for 90 seconds, transfer to a coagulation bath (10% DMF / water solution, 25°C) for 2 minutes and cast membrane. Cut the film to 20cm 2 stand-by. Samples were dried and weighed. The porosity of the polyacrylonitrile hollow membrane prepared according to the method is 0.87, and the maximum mechanical tensile force it can bear is 5.89N. The surface morphology of polyacrylonitrile hollow membrane is as follows: figure 1 .
[0060] (2) Preparation of polyacryloni...
Embodiment 2
[0077] Prepare polyacrylonitrile modified membrane and immobilized lipase according to the method of Example 1, the difference is that the calcium chloride is replaced by glutaraldehyde with a concentration of 0.1, 0.2, 0.5, 1.0, 2.0%, to the polypropylene The lipase immobilized on the nitrile-modified membrane was treated, and the treatment conditions were: room temperature, standing still, 90min.
[0078] According to the method, electrostatic adsorption and chemical cross-linking are combined on the surface of the polyacrylonitrile hollow membrane modified membrane to obtain immobilized lipase.
[0079] The surface morphology of the immobilized lipase prepared by the method of Example 2 is as follows: Figure 12 , after repeated use for 15 times, it can retain more than 60% of the enzyme activity ( Figure 9 ), and stored under different conditions (PBS buffer, tap water, sodium chloride, methylene chloride, ethanol, acetone) for 40h, the enzyme activity is better retained...
Embodiment 3
[0081] Prepare polyacrylonitrile modified membrane and immobilized lipase according to the method of Example 1, the difference is that epichlorohydrin with a concentration of 0.1, 0.5, 1.0, 2.5, 5% is used instead of calcium chloride to treat the polyacrylonitrile The lipase immobilized on the acrylonitrile modified membrane was treated, and the treatment conditions were: 37°C, stirring, 90min.
[0082] According to the method, electrostatic adsorption and chemical cross-linking are combined on the surface of the polyacrylonitrile hollow membrane modified membrane to obtain immobilized lipase.
[0083] The surface morphology of the immobilized lipase prepared by the method of Example 3 is as follows: Figure 13 , after repeated use for 15 times, it can retain more than 55% of the enzyme activity ( Figure 9 ), and stored under different conditions (PBS buffer, tap water, sodium chloride, methylene chloride, ethanol, acetone) for 40h, the enzyme activity is better retained ( ...
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