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Method for improving crop stress resistance by using hydrogen sulfide donor sodium hydrosulfide

A technology of hydrogen sulfide donor and sodium hydrogen sulfide, applied in botany equipment and methods, horticultural methods, pest control, etc., can solve the problems of insufficient application ability of transgenic crops, cumbersome operation, complicated process, etc., and achieve good applicability and social value, the effect of low concentration and simple operation

Inactive Publication Date: 2018-08-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the advent of genetically modified crops, many people have always been skeptical about the safety of genetically modified foods. Genetically modified foods are legally regulated, so the ability of genetically modified crops to participate in the regulation of stress-resistant environments is still insufficient
In addition, the research and development of various growth regulators is also constantly deepening, from a single growth regulator to the mixed use of various regulators, which has also been recognized in the process of anti-adversity research, but in practice In the application, the mixed use of growth regulators is cumbersome and complicated, and may cause environmental pollution to a certain extent

Method used

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  • Method for improving crop stress resistance by using hydrogen sulfide donor sodium hydrosulfide
  • Method for improving crop stress resistance by using hydrogen sulfide donor sodium hydrosulfide
  • Method for improving crop stress resistance by using hydrogen sulfide donor sodium hydrosulfide

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1 Hydrogen sulfide improves seed germination of Sulu No. 2 under drought stress

[0043] A method for improving stress resistance of crops with hydrogen sulfide donor sodium hydrosulfide, the application of sodium hydrogen sulfide as a hydrogen sulfide gas donor in improving the stress-resistant germination of seeds under adverse conditions, exactly before sowing, the seeds are Soak in sodium hydrosulfide solution with a concentration of 0-1.6mmol / L for 6-24 hours at a temperature of 25-30°C.

[0044] Specific steps are as follows:

[0045] 1. Weigh 0.028g of sodium hydrosulfide, dissolve it in 5ml of deionized water to make mother liquor A with a concentration of 100mmol / L, and set it aside.

[0046] 2. Take different volumes of mother liquor A and add different volumes of deionized water according to a certain proportion to dilute it into sodium hydrosulfide solutions with different concentrations of 0-1.6mmol / L.

[0047] 3. Select plump seeds, disinfect wit...

Embodiment 2

[0053] Example 2 Hydrogen sulfide improves seed germination of Sulu No. 2 under salt stress

[0054] Concrete implementation steps are the same as embodiment 1

[0055] In a salt-stressed environment, high concentrations of salt ions can accelerate the entry of harmful ions into the seed embryo. Excessive soil salinity damages the biofilm and disrupts the ion selection of the cell membrane. In addition, salt stress reduces the water potential of the soil environment and prevents the seeds from absorbing water. During seed germination, water needs to be continuously absorbed. When the seeds are under salt stress, the internal and external water potential of the seeds is unbalanced. Due to the low water potential of the soil solution, the water potential difference between the inside and outside of the seed cells decreases, which affects the water absorption of the seeds. The greater the salt concentration, the less water the seeds can absorb, or even be unable to absorb water....

Embodiment 3

[0060] Example 3 Hydrogen sulfide improves seed germination of Sukang No. 3 under drought stress

[0061] Concrete implementation steps are the same as embodiment 1

[0062] In this case, the mung bean variety Sukang No. 3 was used for germination experiments under drought stress, and the sterilized seeds were soaked in sodium hydrosulfide solutions of different concentrations (0, 0.01, 0.05, 0.1, 0.4, 1.6mmol / L)6 ~8h. The seeds are then transferred to glass petri dishes, 40-50 seeds are placed in each dish, 10-20ml of 20% (w:v) PEG-6000 is added thereto as a drought stress condition, and replaced every 6-12h PEG-6000. Observe the seed germination of Sukang 3, count and record the germination rate at 12h and 24h of drought stress, measure the sprout length at 36h, and calculate the germination index. The experiment was repeated three times, with 40-50 seeds in different treatment groups each time. The result is as image 3 And as shown in Table 3:

[0063] Table 3 Seed g...

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Abstract

The invention provides a method for improving crop stress resistance by using hydrogen sulfide donor sodium hydrosulfide. The method for improving the crop stress resistance by using the hydrogen sulfide donor the sodium hydrosulfide includes the steps of: soaking seeds in a sodium hydrosulfide solution with the concentration to be 0.01-1.6 millimole per litre for 6-24 hours and at the temperatureof 25-30 DEG C before sowing; or conducting plant root irrigation with the sodium hydrosulfide solution of the concentration of 0.01-1.6 millimole per litre in a seedling stage and replacing the treatment solution every 6 to 24 hours and replacing continuously for 2 to 5 times; or spraying on the foliage at different stages of stress with the sodium hydrosulfide solution of the concentration of0.01-1.6 millimole per litre during plant maturity. With different application methods and a certain concentration of sodium hydrosulfide solution according to different plants at different growth stages and different periods of stress, the method for improving the crop stress resistance by using the hydrogen sulfide donor the sodium hydrosulfide has the advantages of improving seed germination rate, increasing plant biomass, adjusting the chlorophyll content required for plant photosynthesis, alleviating the problems, caused by the stress, of plant water loss, photosynthetic rate, stomatal conductance, and intercellular carbon dioxide concentration and transpiration rate changes, and improving crops' ability to resist stress.

Description

technical field [0001] The invention relates to the field of crop planting, in particular to a method for improving stress resistance of crops with hydrogen sulfide. Background technique [0002] Abiotic stresses such as drought, salt, and heavy metals are currently the main reasons for the reduction of crop yields around the world. In extreme stress environments, the crops' own ability to resist adversity is extremely insufficient, and their ability to resist stress adjustment cannot meet the requirements of environmental changes. , manifested as a large change in the content of antioxidant substances in plants caused by adversity, such as: drastic changes in the content of endogenous proline, the enzyme activity required in the process of life activities, and the reduction in the content of photosynthetic substances required in the process of plant photosynthesis, etc. , The direct performance is that the growth is obviously inhibited, the plants are short, the leaves turn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/02A01P21/00A01G7/06A01C1/00
CPCA01C1/00A01G7/06A01N59/02
Inventor 谢彦杰武德亮范文越周恒张晶周灿管文学沈杰苏野崔为体沈文飚
Owner NANJING AGRICULTURAL UNIVERSITY
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