High-yield fermentation production method of doramectin

Abstract

The invention provides a high-yield fermentation production method of doramectin. The high-yield fermentation production method comprises the following steps: dissolving a doramectin precursor into water, methanol or ethanol or dispersing the doramectin precursor into a glycerol emulsifying system so as to be convenient for disinfection and material supplementation, wherein the doramectin precursor is cyclohexanecarboxylic acid or sodium cyclohexanecarboxylate; inoculating a strain seed solution into a fermentation culture medium and fermenting; adding the precursor which accounts for 0.02 to0.08 percent of the total weight of the fermentation culture medium at the early period of fermentation for induction; adding the precursor which accounts for 0.18 to 0.22 percent of the total weightof the fermentation culture medium at the middle period of the fermentation; adding the precursor which accounts for 0.02 to 0.08 percent of the total weight of the fermentation culture medium at thelater period of the fermentation. The method is simple and effective, and can be used for rapidly improving a fermentation level of the doramectin; the production cost is reduced and extra equipment and manpower do not need to be increased; the fermentation level of the doramectin can be relatively greatly improved and the production method is relatively suitable for commercial large-scale production.

Description

technical field [0001] The invention belongs to the field of fermentation engineering, and in particular relates to a fermentation production method for increasing the yield of doramectin by optimizing the way of adding precursors. Background technique [0002] Doramectin is a macrolide insecticide synthesized by Pfizer of the United States using the avermectin Streptomyces mutant strain with cyclohexanecarboxylic acid (CHC) precursor. It has a wide range of anti-parasites and significant effects. The route of administration is easy to grasp, high bioavailability, and long drug residual period. It has been used in veterinary clinics in cattle, horses, sheep, goats, pigs, camels, dogs and other mammals. It is also a new spectrum antibiotic selamectin. However, the domestic doramectin fermentation yield is low and the production cost is high, which limits its wide application. [0003] According to literature reports (QA Mckellar, et al, 1996), the biosynthetic pathway of dor...

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign research methods to improve the production of doramectin mainly focus on strain selection, gene mutant strain screening, optimization of fermentation medium formula or improvement of fermentation process. Low, the fermentation level is generally 2000-3000μg / mL, and the fermentation level is relatively unstable

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