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Preparation method of oligosaccharide for promoting proliferation of intestinal probiotics

A technology for intestinal probiotics and oligosaccharides, which is applied in the preparation of oligosaccharides and sugar derivatives, chemical instruments and methods, etc., to achieve the effects of simple production process, expanding the number of users and application fields, and high safety.

Active Publication Date: 2018-08-10
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the preparation and functional activity of isogalacto-oligosaccharides (HeOS) at home and abroad.

Method used

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  • Preparation method of oligosaccharide for promoting proliferation of intestinal probiotics
  • Preparation method of oligosaccharide for promoting proliferation of intestinal probiotics
  • Preparation method of oligosaccharide for promoting proliferation of intestinal probiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Put lactose and melibiose receptors in a screw bottle at a ratio of 1:2, add 50mmol / L phosphate buffer solution with pH 6.5 to adjust the total sugar concentration to 40%, and process under high pressure (121°C, 103Kpa, 20min) , let it completely dissolve, cool naturally to the reaction temperature of 40°C, and keep warm in a water bath for 30 minutes; weigh an appropriate amount of β-galactosidase and add it to the above reaction system to make it reach a final concentration of (10U / mL) and place it on a constant temperature water bath shaker ( 150r / min) for the reaction; after 14 hours of reaction, the boiling water bath was used to inactivate the enzyme for 5 minutes to terminate the reaction; the above reaction product was classified and purified by activated carbon-diatomaceous earth adsorption chromatography to remove monosaccharides and disaccharides, and the purified product was diluted After freeze-drying, a novel functional oligosaccharide is obtained.

[0044...

Embodiment 2

[0048] Put lactose and cellobiose receptors in a screw bottle at a ratio of 1:3, add a 50mmol / L phosphate buffer solution with pH 7.0 to adjust the total sugar concentration to 50%, and process under high pressure (121°C, 103Kpa, 20min) , to make it completely dissolved, then cool naturally to the reaction temperature of 44°C, and keep it warm in a water bath for 30 minutes; weigh an appropriate amount of β-galactosidase and add it to the above reaction system to make it reach a final concentration of (15U / mL) and place it in a constant temperature water bath shaker ( 200r / min) for the reaction; after 12 hours of reaction, the boiling water bath was used to inactivate the enzyme for 5 minutes to terminate the reaction; the above reaction product was classified and purified by activated carbon-diatomaceous earth adsorption chromatography to remove monosaccharides and disaccharides, and the purified product was diluted After freeze-drying, a novel functional oligosaccharide is ob...

Embodiment 3

[0053] Put the lactose and trehalose receptors in a screw bottle at a ratio of 1:4, add a 50mmol / L phosphate buffer solution with pH 7.5 to adjust the total sugar concentration to 60%, and perform high pressure treatment (121°C, 103Kpa, 20min), Let it completely dissolve and cool naturally to the reaction temperature of 42°C, and keep it warm in a water bath for 30 minutes; weigh an appropriate amount of β-galactosidase and add it to the above reaction system to make it reach a final concentration of (20U / mL) and place it in a constant temperature water bath shaker (250r After 10 hours of reaction, the boiling water bath was used to kill the enzyme for 5 minutes to terminate the reaction; the above reaction product was classified and purified by activated carbon-diatomaceous earth adsorption chromatography to remove monosaccharides and disaccharides, and the purified product was diluted Freeze-dry to obtain novel functional oligosaccharides.

[0054] The yield of oligosacchari...

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Abstract

The invention discloses a preparation method of oligosaccharide for promoting the proliferation of intestinal probiotics. According to the method, based on the selection specificity of enzymes on different substrates, lactose, cellobiose, melibiose, trehalose and other general disaccharide oligomers are respectively used as mixed substrates, a transglycosylation reaction is catalyzed with beta-galactosidase derived from Lactobacillus plantarum 70810, and the obtained products are subjected to classification purification through active carbon beta-diatomaceous earth adsorption chromatography soas to obtain various iso galactooligosaccharide mixtures. According to the present invention, the preparation method has characteristics of simple process and low cost; and the content of the oligosaccharides comprising more than three molecules in the oligosaccharides prepared by using the method is more than 95%; and the prepared oligosaccharide provides the significant proliferation activity to intestinal probiotics, can inhibit the growth of harmful bacteria, contains almost no monosaccharides and disaccharides, has the expanded application population and the expanded application field, has high safety, and can be directly added to various foods.

Description

technical field [0001] The present invention relates to the field of production of functional oligosaccharides, more specifically, to a method for preparing oligosaccharides that promote the proliferation of intestinal probiotics. Background technique [0002] A variety of bacteria inhabiting the intestines of humans and animals constitute the intestinal bacterial system. Bifidobacteria and lactic acid bacteria are the few beneficial microorganisms in the human intestinal flora that do not produce endotoxins and exotoxins and are non-pathogenic. A large number of experimental results show that the proliferation of bifidobacteria and lactic acid bacteria in the intestinal tract can increase the body's antibody level, activate the phagocytic activity of macrophages, and improve the ability to resist infection. They are the most beneficial bacteria in the intestinal tract that can promote human health. Since the human gastrointestinal tract lacks an enzyme system to hydrolyze f...

Claims

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Application Information

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IPC IPC(8): C12P19/18C07H3/06C07H1/06
CPCC07H1/06C07H3/06C12P19/18
Inventor 张宏志周剑忠王英刘小莉夏秀东李亚辉魏照辉
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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