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Resistin polypeptide vaccine and application thereof to treatment of mammary cancer

A polypeptide vaccine and vaccine technology, applied in the direction of antibody medical ingredients, medical preparations containing active ingredients, anti-tumor drugs, etc., to achieve obvious effects

Active Publication Date: 2018-08-10
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, genetic vaccines involve the uptake and expression of genes in specific tissues or organs

Method used

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  • Resistin polypeptide vaccine and application thereof to treatment of mammary cancer
  • Resistin polypeptide vaccine and application thereof to treatment of mammary cancer
  • Resistin polypeptide vaccine and application thereof to treatment of mammary cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Preparation of Resistin Protein Vaccine and Detection of Immune Neutralizing Antibody

[0034]In mice, Resistin is mainly secreted by mature adipocytes, and mainly exists in the form of trimers and hexamers in vivo. First, use the B cell antigen epitope prediction analysis software to analyze the region with strong immunogenicity of the Resistin protein sequence, and then use the protein three-dimensional structure analysis software to analyze and predict the region with strong antigenicity, according to the protein monomer and multimer The positions in the 3D structure were selected to synthesize short peptides that were exposed on the surface of the protein 3D structure and had strong antigenicity, and were evaluated in subsequent experiments.

[0035] The 1# polypeptide (N-terminal α-helix, sequence EAIDKKIKQDF) is used as the antigen, and at the same time its C-terminus is coupled to BSA through cysteine ​​to facilitate antigen presentation and enhance imm...

Embodiment 2

[0041] Example 2 Resistin polypeptide vaccine effectively inhibits the growth of breast tumors in C57BL / 6 mice and BALB / c mice under normal diet and high-fat diet-induced obesity model

[0042] Normal diet and diet-induced obese mice were injected subcutaneously with the vaccine five times. The effect of the vaccine on the blood glucose level of mice was detected by blood glucose test strips. Detect the glucose tolerance level and insulin tolerance level of mice respectively, such as Figure 6 and 7 As shown, injection of the vaccine had no effect on the level of glucose tolerance or insulin tolerance in mice. At the same time, the record of body weight changes in the mice throughout the experimental process showed that the body weight of the mice was not affected by the vaccine injection ( Figure 8 ).

[0043] After 6 subcutaneous injections of the vaccine, E0771 breast cancer cells 5*10 were injected into the fat pad of the right second mammary gland of C57BL / 6 mice at ...

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Abstract

The invention discloses a resistin polypeptide vaccine and application thereof to the treatment of a mammary cancer, and belongs to the technical field of biology. A polypeptide vaccine provided by the invention is prepared from a Resistin-protein specific polypeptide segment and an immunologic adjuvant, and becomes the Resistin polypeptide vaccine. Through multiple subcutaneous injections, the vaccine can be used for activating the lymphocyte of an organism, and generates an immune neutralizing antibody aiming at Resistin protein. The Resistin polypeptide vaccine does not influence the sugartolerance and insulin tolerance levels of the organism at all. The Resistin polypeptide vaccine can be used for obviously inhibiting the growth of an ER (Estrogen Receptor) positive mammary tumor anda triple-negative mammary tumor under a mouse model; in addition, the Resistin polypeptide vaccine can be used for obviously inhabiting the growth of the ER positive mammary tumor and the triple-negative mammary tumor of a mouse under an obesity model induced by a high fat diet, and a new way is provided for ameliorating a current treatment method of the mammary cancer by the resistin polypeptidevaccine.

Description

technical field [0001] The invention relates to a resistin polypeptide vaccine and its application in treating breast cancer, belonging to the field of biotechnology. Background technique [0002] Breast cancer is a major problem that plagues women's health around the world. About 1.4 million people around the world are diagnosed with breast cancer every year, and about 500,000 people die from the disease. Breast cancer is the first cause of death for women aged 40-55 . The average annual growth rate of breast cancer in my country is 3%-4%, becoming the cancer with the fastest-growing mortality rate in cities, especially Shanghai, Beijing, Tianjin and coastal areas are high-incidence areas of breast cancer in my country, accounting for female malignant tumors. rate first. [0003] Obesity caused by unreasonable dietary structure and genetic factors is closely related to the incidence of breast cancer. On the one hand, a long-term high-fat diet often leads to an abnormal in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K38/22A61K39/39A61P35/00
CPCA61K38/22A61K39/00A61K39/39A61K2039/575A61P35/00
Inventor 袁增强程金波高宇豪廖亚金
Owner ACADEMY OF MILITARY MEDICAL SCI
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