Preparation of lycopene-rich yeast extract and application of lycopene-rich yeast extract to cosmetics
A technology of yeast extract and lycopene, applied in cosmetics, cosmetic preparations, medical preparations containing active ingredients, etc., can solve problems such as poor stability and poor water solubility, and achieve improved skin elasticity and good development. Value, the effect of reducing water loss
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[0027] The embodiment of the present invention provides a preparation method of lycopene-rich yeast extract, comprising the following steps:
[0028] (1) Strain activation: transfer the Rhodotorula viscosus strain from the storage slope to the activation medium under aseptic conditions;
[0029] (2) Preparation of seed solution: inoculate the activated Rhodotorula viscosus strain from the activated medium into the seed medium for expanded cultivation to obtain the seed solution;
[0030] (3) Liquid fermentation: Inoculate the prepared seed solution into the fermentation medium for liquid fermentation;
[0031] (4) Obtaining of yeast extract: after the fermentation, the fermentation liquid is collected, centrifuged, washed with water, and then processed by enzymatic digestion and microwave crushing.
[0032] In some of these embodiments, the activation medium formula described in the step (1) is: glucose 10-30 g / L, peptone 5-15 g / L, yeast extract powder 5-15 g / L, agar 10 -30 ...
Embodiment 1
[0061] A kind of lycopene-rich yeast extract, its preparation comprises the following steps:
[0062] (1) Strain activation: transfer the Rhodotorula viscosus strain from the storage slope to the activation medium under aseptic conditions; the activation medium formula is: glucose 10 g / L, peptone 15 g / L, yeast Extraction powder 5g / L, agar 30 g / L, natural pH; the seed slant activation culture condition is: culture at 26°C for 60 hours.
[0063] (2) Preparation of seed liquid: the activated Rhodotorula viscosus strain was inoculated from the activated medium into the seed medium for expanded cultivation to obtain the seed liquid; the composition of the seed liquid medium was as follows: Glucose 10 g / L, peptone 15 g / L, yeast extract powder 5 g / L, natural pH, and the rest are deionized water; the specific operation of the expanded culture is as follows: pick 1 ring of culture from the activated slant seeds, and then In a medium containing 40 mL of liquid seed, shake culture at 2...
Embodiment 2
[0067] A kind of lycopene-rich yeast extract, its preparation comprises the following steps:
[0068] (1) Strain activation: transfer the Rhodotorula viscosus strain from the storage slope to the activation medium under aseptic conditions; the activation medium formula is: glucose 30 g / L, peptone 5 g / L, Yeast extract powder 15 g / L, agar 10 g / L, natural pH; the seed slant activation culture condition is: culture at 30° C. for 36 hours.
[0069](2) Preparation of seed liquid: the activated Rhodotorula viscosus strain was inoculated from the activated medium into the seed medium for expanded cultivation to obtain the seed liquid; the composition of the seed liquid medium was as follows: glucose 30 g / L, peptone 5 g / L, yeast extract powder 15 g / L, natural pH, and all the other are deionized water; In a medium containing 20 mL of liquid seed, shake culture at 35°C and 100 rpm for 32 hours to obtain a seed liquid.
[0070] (3) Fermentation: Inoculate the prepared seed solution int...
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