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Plant-derived natural bacteriostatic agent or preservative and application thereof

A natural bacteriostatic agent and preservative technology, applied in the fields of application, food ingredients containing natural extracts, preparations for toiletry, etc., can solve the problem that there is no relevant report on the synergistic effect of antibacterial ingredients on preservatives, etc.

Active Publication Date: 2020-11-06
广州市萃源生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no documents or patents reporting the broad-spectrum antibacterial effect and antibacterial components of edible myrtle fruit, and there are no related reports on the application of antibacterial components and their synergistic effects and synergistic substitution of preservatives

Method used

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  • Plant-derived natural bacteriostatic agent or preservative and application thereof
  • Plant-derived natural bacteriostatic agent or preservative and application thereof
  • Plant-derived natural bacteriostatic agent or preservative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation and acquisition of myrtone compounds from Myrtaceae plant species

[0022] 1.1 Plant material

[0023] Myrtle, a plant of the genus Myrtle in the Myrtaceae family, was used as the experimental material. The plant is widely distributed in southern my country, especially in the south of the Five Ridges. The plant material used in this experiment was collected from Nankang County (District), Ganzhou City, Jiangxi Province. It was identified as R.tomentosa, a plant of the genus Myrtle in the family Myrtaceae, by Wang Faguo, a researcher at the South China Botanical Garden of the Chinese Academy of Sciences. The plant specimens are now deposited in the Natural Products and Chemical Biology Research Laboratory of South China Botanical Garden, Chinese Academy of Sciences.

[0024] 1.2 Experimental Instruments and Reagents

[0025] The optical rotation data were determined by Perkin-Elmer 341 polarimeter (Perkin-Elmer, USA). The ultraviolet spectrum was ...

Embodiment 2

[0032] Embodiment 2: Dihydromyricetin compound is obtained from the isolation of Vitis vinifera species

[0033] 2.1 Plant material

[0034] The plant material was collected from Dayu County, Ganzhou City, Jiangxi Province. It was identified as Ampelopsis grossedentata (Hand.-Mazz.) W.T.Wang by researcher Wang Faguo of the South China Botanical Garden of the Chinese Academy of Sciences. The plant specimens are now deposited in the Natural Products and Chemical Biology Research Laboratory of South China Botanical Garden, Chinese Academy of Sciences.

[0035] 2.2 Experimental Instruments and Reagents

[0036] 1.2 with embodiment 1.

[0037] 2.3 Obtaining the extract

[0038] Weigh 250g of dried leaves of Grape dentatus bud heads, add them to 90% ethanol aqueous solution by volume fraction, soak for half an hour, reflux twice at 55°C for 40 minutes each time; filter while hot, and concentrate under reduced pressure to remove ethanol , placed for 1d, after the pale yellow gran...

Embodiment 3

[0043] Embodiment 3: antibacterial activity detection

[0044] The present invention selects the microdilution method with high sensitivity and can quantitatively detect the bacteriostatic activity of the drug in vitro, that is, the minimum inhibitory concentration (MIC) is used to evaluate the bacteriostatic activity of the extract, compound 1 and compound 2. The minimum inhibitory concentration (MIC) of the compounds was determined by the resazurin chromogenic method. Resazurin is a blue non-fluorescent dye, which can be reduced to a pink fluorescent dye by a variety of reductases in living cells, while inactive cells cannot metabolize it. This method reflects the antibacterial ability of the extract through the change of the color of the bacterial liquid—after co-cultivating the indicator, the extract, and the bacterial liquid for a certain period of time, if the bacterial liquid turns red, it indicates that the compound has no antibacterial activity; Has antibacterial act...

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Abstract

The invention discloses a plant-derived natural bacteriostatic agent or preservative and application thereof. The plant-derived natural bacteriostatic agent or preservative includes a Compound 1 and aCompound 2 or an Extract 1 and an Extract 2; a structural formula of the Compound 1 is shown in Formula 1, and the structural formula of the compound 2 is shown in Formula 2. According to the invention, the Compound 1 (Myrtle ketone) obtained from edible rhodomyrtus tomentosa fruits and the Compound 2 (dihydromyricetin) obtained from ampelopsis grossedentata leaves have synergistic bacteriostastic activity. By separately adding 0.02% of Compound 1 and Compound 2 mixture (weight 40: 60) as a natural Preservative 1 and adding 0.4% of Extract 1 and Extract 2 mixture (weight 50: 50) as a naturalPreservative 2 into cosmetics, the cosmetics can pass a preservative challenge experiment excellently, and the preservative ability of the plant-derived natural bacteriostatic agent or preservative isbasically equivalent to that of chemical preservatives, with good application prospects and development value.

Description

Technical field: [0001] The invention belongs to the field of sterilization and anticorrosion, and in particular relates to a plant-source natural antibacterial agent or antiseptic and application thereof. Background technique: [0002] Preservatives are an indispensable part of cosmetics and food preservation, and their function is to inhibit the growth and reproduction of microorganisms. However, the addition of pure chemical preservatives often has certain safety or irritation problems. For example, long-term use of cosmetics containing chemical preservatives will accelerate skin aging, and some people with special skin types are prone to skin allergic reactions. With the improvement of consumers' requirements for cosmetics and food safety, reducing the application of chemically synthesized preservatives in cosmetics and food, and finding new, effective and safe natural preservatives has become a new development direction for cosmetics and food research. [0003] Myrtle ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/49A61K8/9789A61Q19/00A23L3/3472A23L3/3499
CPCA61K8/498A61K8/9789A61Q19/00A23L3/3499A23L3/3472A61K2800/524A61K2800/5922A23V2002/00A23V2250/21Y02A50/30
Inventor 赵丽云邱声祥杨美珍杜逸琼
Owner 广州市萃源生物科技有限公司
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