Method to promote germination of Polygonatum cyrtonema seeds
A Polygonatum polyflora, seed technology, applied in the direction of seed and rhizome treatment, agriculture, application, etc., can solve the problems of rising prices, unwillingness of pharmaceutical farmers, large market demand, etc., to achieve easier absorption, increase stress resistance, and promote seed germination. Effect
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Embodiment 1-1
[0031] Embodiment 1-1, a kind of method for promoting Polygonatum polyflora seed germination, carries out following steps successively:
[0032] 1) Select Polygonatum multiflora seeds with the same color and size, plump grains, and no mildew scars, and wash them under running water under rubbing conditions for 30 minutes.
[0033] 2), the cleaned seeds obtained in step 1) were first soaked in warm water at 40°C for 24 hours, and then soaked in 1% (mass %) KNO at 40°C. 3 Seeds were soaked in the solution for 24 hours, rinsed with running water three times, and then soaked in 300 mg / L ethephon solution for 12 hours (room temperature).
[0034] 3), the seeds obtained after the treatment in step 2) are placed in a petri dish covered with two layers of wet filter paper, and cultured in a constant temperature incubator at 25° C. in the dark, keeping the filter paper moist.
[0035] The results obtained after culturing for 45 days: the germination rate is 57.8%, the germination pote...
Embodiment 2
[0040] Embodiment 2, a kind of method of promoting Polygonatum polyflora seed germination:
[0041] Cancel the link of soaking seeds for 12h in the ethephon solution of 300mg / L in step 2) of embodiment 1-1;
[0042] The rest are equal to Example 1.
[0043] The results obtained after culturing for 45 days: the germination rate is 46.67%, the germination potential is 32.2%, and the leaf hair rate is 3.3%.
[0044] details as follows:
[0045] The 14th day is dew white, the 20th day root length is 1.7cm, the radicle length of some seeds is about 4cm on the 28th day, the cotyledon is seen on the 35th day, and the number of side buds reaches 5 on the 45th day, the root length is 5.8cm, and the fibrous root The maximum number is 5.
Embodiment 3
[0046] Embodiment 3, a kind of method promoting Polygonatum polyflora seed germination:
[0047] Change the ethephon solution of 300mg / L in embodiment 1-1 step 2) into the gibberellic acid (GA 3 ) solution; all the other are equal to embodiment 1.
[0048] The results obtained after culturing for 45 days are shown in Table 1.
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