Corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of corn continuous-cropping obstacle restoration agent

A continuous cropping obstacle and repair agent technology, applied in the field of microbial and continuous cropping obstacle repair, can solve the problems that restrict the treatment and sustainable development of corn continuous cropping obstacles, soil compaction acidification, secondary salinization, etc., and achieve the purpose of improving yield and fruit quality, Good degradation effect, the effect of promoting stress resistance

Inactive Publication Date: 2018-08-03
SHENYANG AGRI UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

At present, there are relatively few probiotics used in the treatment of corn continuous cropping obstacles and autotoxicity. Most of them use fungicides and chemical fertilizers for soil treatment, resulting in soil compaction and acidification, nutrient imbalance, secondary salinization, etc., and lack of safe and effective biological bacteria treatment. Seriously restricting the governance and sustainable development of corn continuous cropping obstacles, it has become one of the bottleneck problems that need to be solved urgently in corn agricultural production

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  • Corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of corn continuous-cropping obstacle restoration agent
  • Corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of corn continuous-cropping obstacle restoration agent
  • Corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of corn continuous-cropping obstacle restoration agent

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Embodiment Construction

[0014] 1. Materials and Methods

[0015] 1.1 The medium used to isolate Pseudomonas oryzae is an inorganic salt medium, and the formula includes MgSO 4 ·7H 2 O0.2g / L, CaCl 2 2H 2 O 0.02g / L, FeCl 3 0.05g / L, KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, NH 4 NO 3 1.0g / L, cholesterol 0.3g / L, and agar 17g / L respectively with a treatment concentration of 200μg·mL -1 The p-hydroxybenzoic acid, benzoic acid and cinnamic acid are used as the sole carbon source, and the pH value is adjusted to neutral.

[0016] The method for isolating Pseudomonas oryzae: wash the melon rhizome junction with running water, cut it into small pieces, soak in 75% alcohol for 1min, rinse with 0.1% mercury liter for 30s, and rinse it with sterile water for 4 times. Grind it into a slurry in a bowl, dilute the grinding solution 3 times, spread it on an inorganic salt medium, and cultivate it in an incubator at 28°C for 48 hours to obtain a strain of Pseudomonas oryzae.

[0017] Pseudomonas oryzihabitans...

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Abstract

The invention relates to a corn continuous-cropping obstacle restoration agent based on allelopathy autotoxicity and application of the corn continuous-cropping obstacle restoration agent. The corn continuous-cropping obstacle restoration agent is characterized by being prepared from pseudomonas oryzihabitans and burkholderia according to a ratio of (1:3) to (3:1), the concentration of the pseudomonas oryzihabitans in a restoration agent bacteria solution is 2.0 to 3.0*10<8>cfu / ml, and the concentration of the burkholderia in the restoration agent bacteria solution is 2.0 to 3.0*10<8>cfu / ml. The application method of the restoration agent comprises the following steps: adopting a seed coating method, stirring and sufficiently mixing 50g of corn seeds and 5mL of fermented solution, standingfor 3 to 5 hours, and then seeding. The restoration agent adopted by the invention is pseudomonas oryzihabitans and burkholderia, and is good in corn rhizosphere fix planting capability, safe and reliable, good in degradation effect for an autotoxic substance in the continuous cropping corn soil, apparent in effect for promoting the stress resistance of the corn, and capable of increasing the yield of the corns and improving the fruit quality; and the restoration agent is simple in preparation method, reasonable in formula, and capable for controlling the corn continuous cropping obstacles.

Description

technical field [0001] The invention relates to the field of repairing microorganisms and continuous cropping obstacles, in particular to an agent for repairing corn continuous cropping obstacles based on allelopathic autotoxicity and its application. Background technique [0002] Corn is an important field crop in my country. Continuous planting leads to changes in the soil ecological environment of protected areas. Plant residues, root exudates and metabolites of pathogenic microorganisms have a toxic effect on the next crop of corn, affecting seed germination, plant growth, and cell division. , the activity of pathogenic bacteria and the structure of soil microorganisms, thus triggering autotoxicity and resulting in continuous cropping obstacles. [0003] The occurrence of continuous cropping obstacles caused by autotoxicity and root soil-borne diseases can be effectively prevented by adding beneficial microorganisms or antagonistic bacteria. At present, there are relativ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01C12R1/38
CPCC12N1/20A01N63/00
Inventor 高增贵董辉姚远孙艳秋李兴海
Owner SHENYANG AGRI UNIV
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