Isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing process

A technology of IF-1106, I. fumoscens, applied in fungi, biochemical equipment and methods, microorganisms, etc., can solve problems such as differences in biological characteristics of strains, and achieve good application prospects

Inactive Publication Date: 2018-07-31
LULIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the biological characteristics of different strains vary

Method used

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  • Isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing process
  • Isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing process
  • Isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The optimal process for liquid fermentation of I. fumoscens IF-1106 strain, the fermentation steps are as follows:

[0037] Step 1. Take out I. fumoscens IF-1106 strain and activate it, inoculate it on PDA medium, and culture it in a mold incubator at 25°C at constant temperature;

[0038] Step 2, prepare liquid medium, every 50ml liquid medium contains MgSO 4 ·7H 2 O: 0.5g, KCl: 0.5g, KH 2 PO 4 : 1.0g, FeSO 4 ·7H 2 O: 0.1g, sucrose 2.15g and peptone 0.35g, making the sucrose concentration 4.3%, the peptone concentration 0.7%;

[0039] Step 3. After cultivating I. fumigatus IF-1106 bacterial strain in step 1 for 10 days, add Tween 80 sterile water containing 0.1% volume ratio to make a spore suspension, and adjust the concentration to 1×10 7 Spores / ml;

[0040] Step 4: Add the liquid culture medium in step 2 to the petri dish for sterilization, and insert the I. fumigatus IF-1106 bacterial strain in step 3 according to the inoculation amount of 15% volume ratio u...

Embodiment 2

[0043] Effects of Different Carbon and Nitrogen Sources on Mycelia Biomass and Sporulation in Liquid Culture of I. fumoscens IF-1106 Strain

[0044] 2.1 Test method

[0045] Replace the carbon source sucrose in Step 2 of Example 1 with glucose, sucrose, and maltose, respectively, and replace the nitrogen source with peptone and sodium nitrate to form 6 kinds of liquid culture media. Other conditions include 10% inoculum size and 25°C temperature. 1. The shaker speed was 120r / min and 12L / 12D was fermented for 7 days, and the volume ratio of liquid medium to culture dish was 1:5. The mycelial biomass and sporulation of I. fumoscens IF-1106 strain cultured in 6 kinds of liquid medium were counted respectively. The number of spores was checked by microscope, and the hyphae were filtered. The filter paper used in the filtration was dried to constant weight in advance, washed with distilled water, dried in an oven at 60°C, and weighed with an electronic balance.

[0046] 2.2 Test ...

Embodiment 3

[0049] Effects of different concentrations of carbon and nitrogen sources on the mycelial biomass and sporulation of I. fumoscens IF-1106 strain in liquid culture

[0050] 3.1 Test method

[0051] The sucrose concentration in Step 2 of Example 1 was replaced by 2%, 3%, 4%, 5% respectively, and combined with 1% peptone to form 4 kinds of liquid culture medium, and the other conditions were 10% inoculum size and 25°C temperature. ℃, shaker speed of 120r / min and 12L / 12D under the condition of 12L / 12D fermentation culture for 7 days, the volume ratio of liquid medium and culture dish was 1:5. The peptone concentration in Step 2 of Example 1 was replaced by 0.5%, 1.0%, 1.5%, and 2.0%, respectively, and combined into 4 kinds of liquid culture media, and the other conditions were 10% inoculum size, 25°C temperature, and 120r rotation speed of the shaker Under the condition of 12L / 12D / min, the fermentation culture was 7d, and the volume ratio of liquid medium to culture dish was 1:5....

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Abstract

The invention relates to an isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing process. The fermentation steps are as follows: 1, taking out an isaria fumosorosea IF-1106 bacterial strain and activating the isaria fumosorosea IF-1106 bacterial strain; inoculating a PDA culture medium with the isaria fumosorosea IF-1106 bacterial strain and performing culturing in a 25-DEG Cmould incubator; 2, ensuring that each 50ml of liquid culture medium contains 0.5g of MgSO4.7H2O, 0.5g of KCl, 1.0g of KH2PO4, 0.1g of FeSO4.7H2O, crane sugar and peptone, wherein the concentration of the crane sugar is 4.3 percent, and the concentration of the peptone is 0.7 percent; 3, culturing the bacterial strain for 10 days, and adding sterile water containing 0.1 percent of tween 80 to prepare spore suspension solutions, wherein the concentration is adjusted to be 1*10<7> spores/ml; 4, adding a liquid culture medium into a culture dish and carrying out sterilization, inoculating the spore suspension solutions under a sterile condition according to the inoculation quantity of 15 percent by volume ratio, and carrying out fermentation and culturing for 6 to 7 days under whole dark conditions that a temperature is 20 to 30 DEG C and the rotation speed of a shaking table is 150r/min. According to the isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing processdisclosed by the invention, a foundation is laid for large-scale production and industrialized application of the isaria fumosorosea IF-1106 bacterial strain, and the isaria fumosorosea IF-1106 bacterial strain liquid fermentation optimizing process has good application prospects.

Description

technical field [0001] The invention relates to the field of microbial liquid fermentation technology, in particular to the optimization technology of liquid fermentation of I. fumoscens IF-1106 bacterial strain. Background technique [0002] Isaria fumosorosea, belonging to Deuteromycotian, Hyphomycetes, Moniliales, Moniliaceae, Isaria , is an important entomopathogenic fungus with a wide geographical distribution and infects a variety of agricultural and forestry pests, including Bemisia tabaci, Aleurodes vaporariorum, diamondback moth (Plutellaxyllostella), cabbage Caterpillar (Pierisrapae linne), citrus psyllid (Diaphorina dtri) and other agricultural and forestry pests. [0003] Shanxi Agricultural University isolated a strain of I. fumigatus in the field, named IF-1106, and preserved it in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date was April 23, 2013, and the preservation number was CGMCCNo. 7514. P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 田晶弓玉红马瑞燕
Owner LULIANG UNIV
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