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Preparation method of dendrobium huoshanense polysaccharide

A technology of Dendrobium Huoshanense polysaccharides, which is applied in the field of preparation of Dendrobium Huoshanense polysaccharides, can solve the problems of impurity mixing, complex structure and configuration, single method, etc., and achieve the effect of high extraction rate, high purity and excellent drug activity

Active Publication Date: 2018-07-31
河南和生食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of extraction and preparation is too single, which cannot meet the requirements of high-precision and high extraction rate polysaccharide extraction. On the one hand, different types of impurities will be mixed in the extraction process of polysaccharides. On the other hand, polysaccharides also have relatively complex structures and configurations. , these are the reasons why the existing extraction methods still do not achieve the ideal extraction effect

Method used

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  • Preparation method of dendrobium huoshanense polysaccharide
  • Preparation method of dendrobium huoshanense polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A preparation method of Dendrobium Huoshanense polysaccharide, comprising the steps of:

[0028] 1) Pulverization: take Dendrobium huoshanense, wash, freeze-dry, pulverize, superfine pulverize;

[0029] 2) Beating: add the powder obtained in step 1) into acetone and ethanol in turn for beating at 0°C, and the ratio of the added acetone and ethanol to the powder is 1g / 5ml; supernatant;

[0030] 3) Extraction: adding 50 times the weight of hot water to the precipitate obtained in step 2), and ultrasonically extracting; after the extraction is completed, filter and remove the Dendrobium residue, collect the filtrate, and concentrate in vacuum to obtain the polysaccharide extract of Dendrobium huoshanense at a water temperature of 5°C; The ultrasonic power is 1500w, and the time is 50 minutes;

[0031] 4) Alcohol precipitation: adding the extract obtained in step 2) to 20 times by weight of ethanol, beating, and standing to obtain a precipitate. The ethanol is 80% (v / v); ...

Embodiment 2

[0041] A preparation method of Dendrobium Huoshanense polysaccharide, comprising the steps of:

[0042] 1) Pulverization: take Dendrobium huoshanense, wash, freeze-dry, pulverize, superfine pulverize;

[0043] 2) Beating: add the powder obtained in step 1) into acetone and ethanol in turn at 10°C for beating treatment, and the ratio of solid to liquid between the added acetone and ethanol and the powder is 2g / 5ml; supernatant;

[0044] 3) Extraction: adding 80 times the weight of hot water to the precipitate obtained in step 2), and ultrasonically extracting; after the extraction is completed, filter and remove the Dendrobium residue, collect the filtrate, and concentrate in vacuum to obtain the polysaccharide extract of Dendrobium huoshanense at a water temperature of 80°C; The ultrasonic power is 2500w, and the time is 100 minutes;

[0045] 4) Alcohol precipitation: adding the extract obtained in step 2) to 30 times the weight of ethanol, beating, and standing to obtain a ...

Embodiment 3

[0055] A preparation method of Dendrobium Huoshanense polysaccharide, comprising the steps of:

[0056] 1) Pulverization: take Dendrobium huoshanense, wash, freeze-dry, pulverize, superfine pulverize;

[0057] 2) Beating: add the powder obtained in step 1) into acetone and ethanol in turn at 5°C for beating treatment, the ratio of solid to liquid between the added acetone and ethanol and the powder is 1.5g / 5ml; set aside to settle, filter to separate the precipitate with the supernatant;

[0058] 3) Extraction: adding 65 times the weight of hot water to the precipitate obtained in step 2), and ultrasonically extracting; after the extraction is completed, filter and remove the dendrobium residue, collect the filtrate, and concentrate in a vacuum to obtain the polysaccharide extract of Dendrobium huoshanense with a water temperature of 65°C; The ultrasonic power is 2000w, and the time is 75 minutes;

[0059] 4) Alcohol precipitation: adding the extract obtained in step 2) to 2...

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Abstract

The invention discloses a preparation method of a dendrobium huoshanense polysaccharide. The preparation method comprises the following steps of 1), crushing; 2), pulping; 3), extraction; 4), alcoholprecipitation; 5), discoloration treatment; 6), enzymolysis; 7), alcohol precipitation once again; 8), filtration; 9), third-time alcohol precipitation; 10), centrifugal separation; 11), freeze-drying, so as to obtain a dendrobium huoshanense polysaccharide pure product. A raw material adopted by the preparation method is subjected to the freeze-drying and superfine grinding; effective componentscan be prompted to be quickly dissolved out; much water is added in the extraction step for high-temperature extraction; the sufficient extraction of the effective components is facilitated, and a dendrobium huoshanense polysaccharide product obtained by the preparation method provided by the invention is high in extraction rate and high in purity, meanwhile, has excellent drug activity, and has an outstanding advantage in application.

Description

technical field [0001] The invention relates to a method for extracting active ingredients of Chinese herbal medicines, in particular to a method for preparing polysaccharides from Dendrobium huoshanense. Background technique [0002] Dendrobium is the second largest genus in Orchidaceae, which has both ornamental and medicinal value. There are more than 1,400 species in the world, and they are widely distributed in tropical and subtropical regions such as Asia, Europe and Oceania. [0003] region, of which there are about 800 species in my country, mainly distributed in Southwest, East and South China. In my country's traditional medical theory, the fresh or dry stems of various Dendrobium plants are harvested for medicinal purposes, collectively referred to as Herba Dendrobium (herbadendrobe). Dendrobium is a commonly used precious medicinal material. It has the functions of nourishing yin and clearing heat, nourishing the stomach and promoting body fluid, moistening the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/10
CPCA61P3/10C08B37/0003
Inventor 崔胜文秦令祥高愿军
Owner 河南和生食品有限公司
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