Preparation method of dendrobium huoshanense polysaccharide
A technology of Dendrobium Huoshanense polysaccharides, which is applied in the field of preparation of Dendrobium Huoshanense polysaccharides, can solve the problems of impurity mixing, complex structure and configuration, single method, etc., and achieve the effect of high extraction rate, high purity and excellent drug activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] A preparation method of Dendrobium Huoshanense polysaccharide, comprising the steps of:
[0028] 1) Pulverization: take Dendrobium huoshanense, wash, freeze-dry, pulverize, superfine pulverize;
[0029] 2) Beating: add the powder obtained in step 1) into acetone and ethanol in turn for beating at 0°C, and the ratio of the added acetone and ethanol to the powder is 1g / 5ml; supernatant;
[0030] 3) Extraction: adding 50 times the weight of hot water to the precipitate obtained in step 2), and ultrasonically extracting; after the extraction is completed, filter and remove the Dendrobium residue, collect the filtrate, and concentrate in vacuum to obtain the polysaccharide extract of Dendrobium huoshanense at a water temperature of 5°C; The ultrasonic power is 1500w, and the time is 50 minutes;
[0031] 4) Alcohol precipitation: adding the extract obtained in step 2) to 20 times by weight of ethanol, beating, and standing to obtain a precipitate. The ethanol is 80% (v / v); ...
Embodiment 2
[0041] A preparation method of Dendrobium Huoshanense polysaccharide, comprising the steps of:
[0042] 1) Pulverization: take Dendrobium huoshanense, wash, freeze-dry, pulverize, superfine pulverize;
[0043] 2) Beating: add the powder obtained in step 1) into acetone and ethanol in turn at 10°C for beating treatment, and the ratio of solid to liquid between the added acetone and ethanol and the powder is 2g / 5ml; supernatant;
[0044] 3) Extraction: adding 80 times the weight of hot water to the precipitate obtained in step 2), and ultrasonically extracting; after the extraction is completed, filter and remove the Dendrobium residue, collect the filtrate, and concentrate in vacuum to obtain the polysaccharide extract of Dendrobium huoshanense at a water temperature of 80°C; The ultrasonic power is 2500w, and the time is 100 minutes;
[0045] 4) Alcohol precipitation: adding the extract obtained in step 2) to 30 times the weight of ethanol, beating, and standing to obtain a ...
Embodiment 3
[0055] A preparation method of Dendrobium Huoshanense polysaccharide, comprising the steps of:
[0056] 1) Pulverization: take Dendrobium huoshanense, wash, freeze-dry, pulverize, superfine pulverize;
[0057] 2) Beating: add the powder obtained in step 1) into acetone and ethanol in turn at 5°C for beating treatment, the ratio of solid to liquid between the added acetone and ethanol and the powder is 1.5g / 5ml; set aside to settle, filter to separate the precipitate with the supernatant;
[0058] 3) Extraction: adding 65 times the weight of hot water to the precipitate obtained in step 2), and ultrasonically extracting; after the extraction is completed, filter and remove the dendrobium residue, collect the filtrate, and concentrate in a vacuum to obtain the polysaccharide extract of Dendrobium huoshanense with a water temperature of 65°C; The ultrasonic power is 2000w, and the time is 75 minutes;
[0059] 4) Alcohol precipitation: adding the extract obtained in step 2) to 2...
PUM
![No PUM](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap