Method for adopting oryza eichingeri young embryos for breeding regeneration plants

A technology for regenerating plants and wild rice, applied in the field of plant tissue culture, can solve problems such as no public reports, and achieve the effect of solving insufficient resources, convenient operation, and short cycle

Inactive Publication Date: 2018-07-31
云南省农业科学院生物技术与种质资源研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a method for breeding regenerated plants with immature embryos of wild rice with tight ears. After searching the literature, there is no public report with the same content as the present invention.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] In this example, young embryos of Oryza japonica were used as experimental materials, and regenerated plants were obtained by the method of the present invention. The implementation process is as follows:

[0019] a. Aseptic treatment of explants: sterilize the immature immature embryos in the embryo organ primordia differentiation stage 7 days or 8 days or 9 days after the flowering of the wild rice with a volume percentage of 75% alcohol for 30 seconds, Then use 10% sodium hypochlorite by volume percentage to sterilize for 15 minutes, shake it well during the period, pour off the sodium hypochlorite, wash 6 times with sterile water, take out the cleaned immature embryos and dry them with sterile absorbent paper;

[0020] b. Callus induction culture: transfer the cleaned immature embryos to the callus induction medium, place them in an artificial climate box at 28°C and culture them in the dark for 30 days, grow transparent sticky cell clusters, and continue to culture...

Embodiment 2

[0027] Embodiment two is basically the same as embodiment one. The difference is that the experimental material in Example 2 is the immature embryo of Uganda's wild rice.

[0028] Practical application shows that the present invention not only breaks through the problem of breeding offspring by seeds, but also has high callus induction rate and seedling growth rate of the mature seeds of wild rice with tight ears, and is an efficient method for obtaining a large number of offspring of wild rice with tight ears.

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PUM

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Abstract

The invention relates to a method for adopting oryza eichingeri young embryos for breeding regeneration plants, and belongs to the technical field of plant tissue culture. The method comprises the steps of explant selection, explant sterilization, callus induction and enrichment culture, callus differentiation culture, differentiated seedling rooting culture, seedling hardening and transplanting.By applying different plant hormone combinations and culture condition combinations, the oryza eichingeri callus is successfully induced, the callus is transferred to an enrichment medium for enrichment culture, the callus quality is improved, and the callus number is increased. The method has the advantages that oryza eichingeri regeneration plants can be efficiently obtained, the breeding speedis high, the period is short, the problem that oryza eichingeri resources are not sufficient is solved, and the method is of great significance in protecting endangered oryza eichingeri resources.

Description

technical field [0001] The invention relates to a method for breeding regenerated plants with immature embryos of wild rice with tight ears, and belongs to the technical field of plant tissue culture. Background technique [0002] Oryza eichingeri (2n=24) is native to Africa and Sri Lanka. Its karyotype is CC and its genome size is 686MB, which is 1.6 times that of Asian cultivated rice Oryza sativa. Tight-eared wild rice has many excellent features that cultivated rice does not have, such as resistance to brown planthopper, white-backed planthopper, black-tailed leafhopper, stem borer and bacterial blight. Tight panicle wild rice resources have excellent characteristics and have become a treasure house for rice breeding. [0003] At present, the propagation methods of wild rice with tight ears are mainly stem cuttings and root division transplanting, but the reproduction rate is low and the reproduction time is long, which limits the breeding process. At the same time, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王玲仙付坚陈玲程在全柯学张敦宇陈越钟巧芳曾民殷富有余腾琼王波肖素勤雷涌涛蒋聪赵才美
Owner 云南省农业科学院生物技术与种质资源研究所
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