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Creation and application of anti-hepatitis B model based on host cell targets

An anti-hepatitis B, model technology, applied in the determination/inspection of microorganisms, the introduction of foreign genetic material using vectors, biochemical equipment and methods, etc., can solve the problem of lack of specific target molecular models, etc. strong effect

Inactive Publication Date: 2018-07-10
CHINA JILIANG UNIV
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Problems solved by technology

In view of the fact that there is no cure drug and method for HBV infection, and there is a lack of specific targets and reliable molecular models

Method used

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Examples

Experimental program
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Embodiment 1

[0010] Example 1: Construction and evaluation of an anti-HBV model based on host cell targets

[0011] 1 Prepare the following materials:

[0012] 1.1 Human HL-7702 hepatocytes: purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.

[0013] 1.2 Oxymatrine: product of Suzhou Baozetang Pharmaceutical Technology Co., Ltd.

[0014] 2 The construction method is as follows:

[0015] 2.1 Human HL-7702 hepatocytes were routinely cultured in 1640 medium containing 10% fetal bovine serum, 100 IU / m penicillin and 100 μg / mL streptomycin at 37°C and 5% CO2.

[0016] 2.2 Using bimolecular fluorescence complementation technology, using the self-cleavage translation property of virus-derived 2A polypeptide, the human HO-1 gene, HBV protein gene, and two non-fluorescence complementary fragments VN155 and VC155 of the fluorescent protein Venus were constructed in a single on the carrier.

[0017] 2.3 After the HL-7702 hepatocytes adhered t...

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Abstract

The invention relates to creation and application of an anti-hepatitis B model based on host cell targets. Heme oxygenase-1 is adopted as a target, human heme oxygenase-1 genes and hepatitis B virus protein genes are transferred into liver cells through carriers for expression, and simultaneous targeting of heme oxygenase-1 and a creation program and evaluation method of an anti-hepatitis B virusmedicine evaluation model based on interaction between heme oxygenase-1 and hepatitis B virus protein are achieved. Especially, the host cell components are adopted as the targets, the anti-hepatitisB in-vitro model with high specificity and excellent pertinence is generated, and the application of the model in research on hepatitis pathogenesis mechanisms, drug therapies and research and development of innovative drugs is achieved.

Description

technical field [0001] The present invention relates to a method for establishing an evaluation model of an anti-hepatitis B virus drug, in particular to an in vitro model for a host cell target and its operation procedure to produce a specific anti-hepatitis B virus molecular model and its effect on B Applications in the research of hepatitis pathogenesis, drug treatment and innovative drug development. Background technique [0002] Hepatitis B is a very harmful worldwide epidemic infectious disease caused by hepatitis B virus (HBV). At present, there are about 350 million HBV carriers in the world, and China is a high-infection area of ​​HBV, and HBV carriers account for The proportion of the total population is as high as 10%, among which there are about 30 million chronic hepatitis B patients, and about 350,000 people die of chronic hepatitis B related diseases every year. Although some anti-hepatitis B drugs developed at home and abroad can inhibit virus replication to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12Q1/686G01N33/573
CPCC12N15/85C12Q1/686G01N33/573G01N2333/90245C12Q2521/107
Inventor 伍义行张昺燚关荣发
Owner CHINA JILIANG UNIV
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