Hypsizygus marmoreus strain and culture method thereof
The technology of the mushroom and the strain is applied in the field of the strain of the mushroom and its cultivation, which can solve the problems of long cultivation period, unsatisfactory mushroom type, long cultivation period, etc., and achieves excellent morphological characteristics, short cultivation period, biological The effect of stable academic characteristics
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Embodiment 1
[0028] Embodiment 1 A kind of method of cultivating the mushroom strain WZ-29
[0029] The present embodiment discloses a method for cultivating jitake mushroom strain WZ-29, the method comprising the following steps:
[0030] (1) Bacteria culture
[0031] Inoculate the mushroom strain WZ-29 in the culture medium for cultivation, and the cultivation conditions are as follows: the temperature is 20-25°C; the relative air humidity is 70-80%; CO 2 The concentration is 2000-3000ppm, cultivated for 30-38 days to obtain mycelia;
[0032] (2) Cultivation of mycelium
[0033] The mycelia obtained in the step (1) is inoculated in the fruit body medium for cultivation, and the cultivation conditions are: the temperature of the cultivation room is 20-28°C; the relative air humidity is 70-80%; CO 2 The concentration is 2500-4000ppm, cultivated for 70-78 days to obtain cultivated mycelium;
[0034] (3) Cultivate and produce fruiting bodies
[0035] The cultivated mycelium obtained in ...
Embodiment 2
[0040] Embodiment 2 A kind of true mushroom
[0041] Comprehensively considering aspects such as strain yield, stability and uniformity, the jiji mushroom produced according to the cultivation method of Example 1 is screened to obtain an optimal strain, which is named jiji mushroom strain WZ-29 . The mushroom strain WZ-29 has been preserved in the Guangdong Provincial Microbial Culture Collection Center on November 8, 2017. Its preservation number is GDMCCNO: 60276, and its biological classification is named Hypsizygus marmoreus WZ-29. The jitake mushroom produced by the jitake mushroom strain has the following characteristics:
[0042] The color of the spots in the center of the mushroom cap is light brown, and the color gradually fades towards the edge. The spots are evenly distributed in the center and regularly distributed, such as figure 1 As shown: the flesh is thick and crisp, without cracks; the hyphae are dense and the aerial hyphae are well developed; the gills are...
Embodiment 3
[0045] In this embodiment, the comparison between the jitake mushroom strain WZ-29 in the present invention and the jijitake mushroom strain Finc-W-227 in the prior art is carried out. These two bacterial strains are all cultivated according to the culture condition in embodiment 1, and experimental result is as follows:
[0046] Table 1
[0047]
[0048]Taking Finc-W-227 as the control, set 6 bacterial ages from 70d (that is, days, the same below) to 95d, each increment of 5d is a bacterial age, and each bacterial age is 80 bottles. The volume of the cultivation bottle is 850cc, and the diameter of the bottle mouth is 58mm.
[0049] The results of this experiment are shown in Table 1. The highest yield per bottle of Finc-W-227 is 228g / bottle, and the optimum bacterial age is 90d. The highest yield per unit of WZ-29 is 250g / bottle (that is, gram / bottle, the same below), and the optimal bacterial age is 90d. Through comparison, it can be seen that WZ-29 has obvious advant...
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