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Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer

A technology of tert-butyl cup and modified electrode, which is applied in the fields of electrochemical analysis and biology, and achieves the effects of environmental protection and pollution-free preparation process, simple and easy method, and efficient identification.

Inactive Publication Date: 2018-07-06
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the published literature so far, the use of 4-tert-butylcalix[4]arene modified electrodes for electrochemical chiral recognition is rarely reported.

Method used

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  • Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer
  • Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer
  • Preparation method of 4-tertiary butyl calix(4)arene modified electrode applied to electrochemical method to recognize amino acid enantiomer

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The preparation of 4-tert-butylcalix[4]arene modified electrode for electrochemical recognition of tryptophan enantiomers includes the following steps:

[0024] (1) Grinding 4-tert-butylcalix[4]arene into powder and ultrasonically dispersing it in ultrapure water to prepare a 3 mM 4-tert-butylcalix[4]arene dispersion. 10 μL was drip-coated on the surface of the glassy carbon electrode, and dried at room temperature to obtain a 4-tert-butylcalix[4]arene-modified electrode.

[0025] (2) A three-electrode system was used in the experiment, the 4-tert-butylcalix[4]arene modified electrode was used as the working electrode, the platinum plate electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode. The three-electrode system was respectively immersed in the prepared In 1.0mM L- / D-tryptophan solution, after standing still for 90s, within the potential range of 0.4-1.2V, conduct differential pulse test at a scan rate...

Embodiment 2

[0027] The preparation of 4-tert-butylcalix[4]arene modified electrode for electrochemical recognition of tyrosine enantiomers includes the following steps:

[0028] (1) Grinding 4-tert-butylcalix[4]arene into powder and ultrasonically dispersing it in ultrapure water to prepare a 3 mM 4-tert-butylcalix[4]arene dispersion. 10 μL was drip-coated on the surface of the glassy carbon electrode, and dried at room temperature to obtain a 4-tert-butylcalix[4]arene-modified electrode.

[0029] (2) A three-electrode system was used in the experiment, the 4-tert-butylcalix[4]arene modified electrode was used as the working electrode, the platinum plate electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode. The three-electrode system was respectively immersed in the prepared In 1.0mM L- / D-tyrosine solution, after standing still for 90s, in the potential range of 0.4-1.2V, conduct differential pulse test at a scan rate of 8mV / ...

Embodiment 3

[0031] Different concentrations of 4-tert-butylcalix[4]arene modified electrodes to identify tryptophan enantiomers include the following steps:

[0032] (1) Grind 4-tert-butylcalix[4]arene into powder and ultrasonically disperse it in ultrapure water to prepare 4-tert-butylcalix[4]arene dispersions with different concentrations. The experimental concentration is 1.0-5.0 mM. 10 μL of 4-tert-butylcalix[4]arene dispersion liquid with different concentrations was taken drop-coated on the surface of the glassy carbon electrode, and dried at room temperature to obtain a 4-tert-butylcalix[4]arene modified electrode.

[0033] (2) A three-electrode system was used in the experiment. The electrode modified with different concentrations of 4-tert-butylcalix[4]arene was used as the working electrode, the platinum plate electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode. The three-electrode system was respectively Immerse ...

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Abstract

The invention relates to a preparation method of a 4-tertiary butyl calix(4)arene modified electrode applied to an electrochemical method to recognize an amino acid enantiomer. The preparation methodcomprises the following steps of preparing a 4-tertiary butyl calix(4)arene modified electrode and recognizing the amino acid enantiomer through the electrochemical method. The preparation method disclosed by the invention has the beneficial effects that the 4-tertiary butyl calix(4)arene modified electrode preparation method is simple, convenient, easy and practical, a preparation process achieves environmental friendliness and has no pollution, and furthermore, the modified electrode can recognize the amino acid enantiomer in high efficiency.

Description

technical field [0001] The invention relates to a preparation method of a 4-tert-butylcalix[4]arene modified electrode used for electrochemically identifying amino acid enantiomers, and belongs to the fields of electrochemical analysis and biotechnology. technical background [0002] Amino acids are the basic units of biologically functional macromolecular proteins, and proteins are the main bearers of life activities. The molecular structures of enantiomers in different configurations are mirror images of each other but cannot be superimposed. They have the same physical properties but exhibit completely different pharmacological activities, metabolic processes and toxicological effects in vivo. Therefore, the identification and analysis of amino acid enantiomers is established. Methods play a very important role in life sciences, medicinal chemistry, and human health. At present, there are many technologies for chiral recognition of amino acids. Electrochemical methods ha...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 孔泳杨嘉佩谭文胜
Owner CHANGZHOU UNIV
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