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Degradation agent of aflatoxin, preparation method and application thereof

An aflatoxin and degrading agent technology, applied in the field of aflatoxin degradation, can solve the problems of poor stability, incomplete extraction and high cost of solvent extraction, and achieve the effects of safe degradation and mild reaction.

Active Publication Date: 2018-07-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high cost of solvent extraction, incomplete extraction, poor stability of effect, and safety in each method, it is difficult to be widely used.

Method used

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  • Degradation agent of aflatoxin, preparation method and application thereof
  • Degradation agent of aflatoxin, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Activation of strains

[0033] Inoculate Bacillus amyloliquefaciens in the activated medium for cultivation at a temperature of 30°C for 24 hours; inoculate the activated strains in a fermenter, control the temperature at 30°C and culture at 180rpm for 18 hours, collect the bacteria by centrifugation, add sterile Resuspend bacteria in water to a concentration of 10 10 cfu / mL.

[0034] The above activation and fermentation medium is NB medium (g / L): beef extract 5.0, peptone 10.0, sodium chloride 5.0, pH 7.0.

[0035] The Bacillus amyloliquefaciens was purchased from Shandong Junde Biotechnology Co., Ltd.

[0036] Inoculate Saccharomyces cerevisiae in the activated medium and cultivate it at 28°C for 20 hours; inoculate the activated strains in a fermenter, ferment for 48 hours at 28°C and 150rpm, collect the cells by centrifugation, add sterile water to resuspend the cells , so that the concentration reaches 10 8 cfu / mL.

[0037] The above activation and fermentati...

Embodiment 2

[0040] The aflatoxin degradation agent is prepared from 60g of fresh aloe vera, 75g of fresh lemon fermented liquid, 1% by weight of peppermint oil, 0.5% by weight of laccase and 0.2% by weight of Tween-80.

[0041] (1) Fresh aloe vera 60g, fresh lemon 75g, squeeze the juice, filter; the juice residue is crushed to 40 mesh, add 1.5 times the weight of inorganic salt solution, stir well; add 1.5% glucose to the juice, stir well; juice residue and juice Steam sterilize for 15 minutes and cool to room temperature;

[0042] The inorganic salt solution (g / L): (NH 4 ) 2 SO 4 2.0, KH 2 PO 4 2.0, MgSO 4 0.3, NaCl 0.5, FeSO 4 0.005, MnSO 4 0.016, ZnCl 2 0.017, CoCl 2 0.002.

[0043] (2) Add Bacillus amyloliquefaciens bacterial liquid to the juice residue after cooling, make Bacillus amyloliquefaciens concentration ≥ 10 9 cfu / mL, cultured at 37°C for 48 hours;

[0044] (3) Add Saccharomyces cerevisiae liquid to the cooled juice to make the concentration of Saccharomyces cere...

Embodiment 3

[0047] The aflatoxin degradation agent is prepared from 80g of fresh aloe vera, 100g of fresh lemon fermented liquid, 1% by weight of peppermint oil, 0.3% by weight of laccase, and 0.2% by weight of Tween-80.

[0048] (1) Fresh aloe vera 80g, fresh lemon 100g, squeeze the juice, filter; the juice residue is crushed to 40 mesh, add 1.5 times the weight of inorganic salt solution, and stir well; add 1.5% glucose to the juice, stir well; juice residue and juice are mixed at 100°C Steam sterilize for 15 minutes and cool to room temperature;

[0049] The inorganic salt solution (g / L): (NH 4 ) 2 SO 4 2.0, KH 2 PO 4 2.0, MgSO 4 0.3, NaCl 0.5, FeSO 4 0.005, MnSO4 0.016, ZnCl 2 0.017, CoCl 2 0.002.

[0050] (2) Add Bacillus amyloliquefaciens bacterial liquid to the juice residue after cooling, make Bacillus amyloliquefaciens concentration ≥ 10 9 cfu / mL, cultured at 37°C for 48 hours;

[0051] (3) Add Saccharomyces cerevisiae liquid to the cooled juice to make the concentrati...

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Abstract

The invention discloses a degradation agent of aflatoxin, a preparation method and application thereof, and belongs to the technical field of aflatoxin degradation. The degradation agent of the aflatoxin is prepared from the following components: 60-100 g of fresh aloe, 75-125 g of fermentation liquor of fresh lemon, 1% of oleum menthae, 0.1-0.5% of laccase, and 0.2% of Tween-80. Through the mutual coordination among the components, the aflatoxin is adequately degraded. The degradation agent of the aflatoxin is safe in aflatoxin degradation, high-efficient, and mild in reaction conditions, andis capable of degrading the aflatoxin by 96% or above.

Description

technical field [0001] The invention belongs to the technical field of aflatoxin degradation, and in particular relates to an aflatoxin degradation agent, a preparation method and an application thereof. Background technique [0002] Aflatoxin is known for its toxicity, carcinogenicity, teratogenicity, and mutagenicity. It is a secondary metabolite produced by fungi such as Aspergillus flavus and Aspergillus parasiticus. It is essentially a derivative of dihydrofuranocoumarin. It is carcinogenic The source of the properties is the coumarin mechanism and the source of the toxicity is the dihydrofuran ring structure. Aflatoxin is seriously polluted in food and feed, and poses a great threat to the health of humans and livestock. Human eating food containing aflatoxin can lead to acute poisoning of aflatoxin, liver damage, and even liver cancer. Livestock consumption of aflatoxin-contaminated feed can lead to reduced feed intake, production performance, and immune function. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12P1/04A23L5/20C12R1/07C12R1/865
CPCA23V2002/00A23L5/27C12P1/02C12P1/04C12N1/185C12N1/205C12R2001/07C12R2001/865A23V2250/762
Inventor 杨庆利孔奕凡于春娣唐娟赵海燕
Owner QINGDAO AGRI UNIV
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