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Metabolomics profiling of central nervous system injury

A technology of central nervous system and metabolomics, applied in omics, analytical materials, biostatistics, etc., can solve problems such as lack of sensitivity

Active Publication Date: 2018-06-29
LONDON HEALTH SCI CENT RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The former measures a large number of metabolites but lacks sensitivity (micromolar range), while the latter is very sensitive for quantitative amino acids, acylcarnitines, glycerophospholipids, sphingolipids, and sugars (picomolar range)

Method used

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  • Metabolomics profiling of central nervous system injury
  • Metabolomics profiling of central nervous system injury
  • Metabolomics profiling of central nervous system injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Materials and methods

[0097] The Western University Human Research Ethics Committee approved this study. Informed consent was obtained from the legal guardian, and consent was obtained from the adolescent subjects.

[0098] Target recruitment:

[0099] Male youth ice hockey players (bantamweight, age 12-14 years) from southwestern Ontario, Canada were recruited for this study. To aid in recruitment, informational posters of the study were presented in the ice hockey city of Arenas along with consent forms from Arenas officials, and oral presentations have been made to several area hockey boards and coaches. Juvenile hockey players with suspected concussion injuries were screened and consent was obtained at the primary physician at the Fowler Kennedy Sports Medicine Clinic at Western University. A sports concussion injury is diagnosed when the mechanism of injury is observed after the onset of typical concussion symptoms and in the absence of structural damage. C...

Embodiment 2

[0170] Example 2 - Primary Impact Traumatic Brain Injury

[0171] Materials and methods

[0172] shock exposure

[0173] In performing this study, the authors followed the Guidelines for the Care and Use of Laboratory Animals and the Ethics of Animal Experimentation published by the Canadian Society for the Care of Animals. Adult male Sprague-Dürer rats were obtained from Charles River Laboratories (St. Constant, Que, Canada) and acclimatized for at least one week prior to exposure. A total of 15 control samples and 15 impact-injured samples. On the day of use, anesthetize the animal (~280-330 g) with 3% isoflurane in oxygen, place it in a restraint consisting of a clear plastic cylindrical sleeve, with the neck comfortably enclosed in a plastic ring, And the head protrudes through an opening at one end which is concave so that it conforms to the curvature of the inside of the blast tube. Use end caps fitted with pistons to support the rear legs. On the left side of the...

Embodiment 3

[0196] Example 3 - Prognosis

[0197] Metabolomics

[0198] Plasma from participants in three groups was analyzed: (1) participants with a first concussion injury, (2) participants with two or more reported concussion injuries, and (3) no history of concussion injury comparison participants. All concussion-injured and non-concussion-injured controls were clinically evaluated to determine concussion symptoms and severity. Participants were also assessed according to accepted standard diagnostic criteria. Plasma was analyzed for metabolites by DI / LC-MS / MS and MR.

[0199] PCA

[0200] Using PCA, it was demonstrated that the top 10 components, each weighted to a number of underlying metabolites, explained the majority of the variance in the data.

[0201] t-SNE

[0202] Using t-SNE, the entire metabolic dataset was reduced to two dimensions, since the inherent dimensionality of this data is significantly lower than the number of metabolites. After this dimensionality...

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Abstract

A method of diagnosing central nervous system injuries such as acquired brain injury (ABI) and / or acquired spinal cord injury (ASI), including mild TBI (concussion or blast wave), mild ASI (contusion,stretch or partial cord transection), non-TBI brain injury and / or non-TSI spinal cord injury in a subject (animal or human). The method includes (a) obtaining a biological test sample from the subject, identifying metabolites in the subject's sample using metabolomics thereby obtaining a subject's metabolite matrix and generating a subject's profile using the patient's metabolite matrix; and (b)using multivariate statistical analysis and machine learning to compare the subject's profile with predetermined set of profiles of CNS injuries and a predetermined set of profiles of controls to determine if the subject has a CNS injury.

Description

technical field [0001] The field of the invention relates to metabolomics and methods for diagnosing injuries of the central nervous system (CNS), including all cerebrospinal cord injuries. More specifically, the present invention relates to metabolomics and methods for the diagnosis of mild traumatic brain injury, including concussion and impact injury; mild spinal cord injury, including spinal cord hyperflexion, hyperextension, rotation, lateral stress, Injuries from compression, traction, and partial transection of the spinal cord; and from stroke, poisoning, psychological distress, chemicals, infection, inflammation, autoimmune disease, degenerative processes, hypoxia, ischemia, metabolic derangement, and cancer / radiation Non-traumatic CNS injury. Background technique [0002] Acquired brain injury (ABI) and acquired spinal cord injury (ASI) are brain and spinal injuries resulting from events occurring in utero, perinatally, and postpartum, respectively. These injuries...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N33/68G06F19/24G16B40/20G16B40/30
CPCG01N33/483G01N2570/00G01N2800/28G01N2800/2871G01N24/08G01N33/6896G16C20/70G16B40/30G16B40/20G16B40/10G16B40/00G01N33/487
Inventor 道格拉斯·戴尔·弗雷泽罗伯特·巴尔塔亚瑟·布朗塔尼娅·查里克·斯图尔特马克·戴利格雷格·A·德卡班恩蒂莫西·多尔蒂丽萨·费舍尔杰夫·福尔摩斯拉维·梅农托尼·鲁帕尔J·凯文·舒梅克
Owner LONDON HEALTH SCI CENT RES
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