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Micro-ecological preparation as well as preparation method and application thereof

A technology of microecological preparations and composite microecology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria used in food preparation, etc., can solve problems such as side effects, hazards of animal husbandry products, threats to human health, etc., and achieve growth Fast speed, increase disease resistance, and improve the effect of intestinal flora balance of livestock and poultry

Inactive Publication Date: 2018-06-29
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The application of antibiotics in the field of animal husbandry has greatly promoted the development of animal husbandry scale and modernized farming. However, due to the abuse of antibiotics, various side effects have occurred, especially the residues of antibiotics in livestock and poultry products, which directly threaten Human health, potential hazards posed by livestock products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The microecological preparation of the present embodiment adopts a single Lactobacillus acidophilus bacterial powder, and its number of live bacteria is 2.1×10 11 CFU / g-6.0×10 11 CFU / g.

[0024] Preparation of Lactobacillus acidophilus powder:

[0025] Add 1% glucose, 0.2% casein hydrolyzate, 0.1% sodium acetate and 0.25% diammonium hydrogen citrate to the Tribulus terrestris extract, and then add acidophilus milk at a volume ratio of 2%. Bacillus, cultured at 37°C for 18 hours, centrifuged at 4°C, 6000rpm for 20 minutes to obtain Lactobacillus acidophilus sludge, then added a compound freeze-drying protective agent 3 times the weight of the sludge, and dried in a vacuum freeze dryer for 24 hours to obtain The number of live bacteria is 3.0×10 11 CFU / g-6.0×10 11 CFU / g of Lactobacillus acidophilus powder;

[0026] Each 100 g of the compound freeze-drying protective agent contains 5 g of phytase, 15 g of galacto-oligosaccharides, 5 g of jujube polysaccharide, 3 g of ...

Embodiment 2

[0029] The micro-ecological preparation of the present embodiment adopts single Paulady yeast bacterium powder, and its number of viable bacteria is 3.0×10 10 CFU / g-5.0×10 10 CFU / g.

[0030] Preparation of Paulady Yeast Powder:

[0031] Fresh dates were washed and beaten to obtain jujube pulp, and pectinase, cellulase and hemicellulase were added to the jujube pulp with 1% of the mass of the jujube pulp, incubated at 60°C for 2 hours, and then suction filtered to obtain Jujube juice, after cooling to 38°C, add 1% activated Pauladicap according to the volume ratio, after cultivating at 37°C for 28 hours, centrifuge at 4°C, 4000rpm for 20min to collect the Pauladicial yeast sludge, and then add The composite freeze-drying protective agent with 2 times the weight of the bacteria slime was placed in a vacuum freeze dryer for 24 hours to freeze-dry, and the number of viable bacteria was 3.0×10 10 CFU / g-5.0×10 10 CFU / g of Paulady yeast powder;

[0032] The 100 g compound freeze...

Embodiment 3

[0035] The microecological preparation of this embodiment is a compound microecological preparation consisting of Pauladic yeast powder and Lactobacillus acidophilus powder in a mass ratio of 1:1, wherein the number of viable bacteria in Pauladic yeast powder is 1.0× 10 10 CFU / g-2.5×10 10 CFU / g, live bacteria count of Lactobacillus acidophilus powder is 1.0×10 11 CFU / g-3.0×10 11 CFU / g.

[0036] 1) Preparation of Paulady yeast powder:

[0037] Fresh dates were washed and beaten to obtain jujube pulp, and pectinase, cellulase and hemicellulase were added to the jujube pulp with 1% of the mass of the jujube pulp, incubated at 60°C for 2 hours, and then suction filtered to obtain Jujube juice, after cooling to 38°C, add 1% activated Pauladicap according to the volume ratio, after cultivating at 37°C for 28 hours, centrifuge at 4°C, 4000rpm for 20min to collect the Pauladicial yeast sludge, and then add The composite freeze-drying protective agent with 2 times the weight of th...

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Abstract

The invention provides a micro-ecological preparation as well as a preparation method and application thereof. The micro-ecological preparation is prepared from single lactobacillus acidophilus bacterium powder and saccharomyces boulardii bacterium powder or a compound micro-ecological preparation of the two; the micro-ecological preparation is added into livestock and poultry feed for pigs, cattle, sheep and chickens according to the mass ratio of 0.05 percent to 0.2 percent and is uniformly mixed with the feed to prepare micro-ecological preparation feed. According to the micro-ecological preparation provided by the invention, lactobacillus acidophilus and saccharomyces boulardii can be used for improving intestinal flora imbalance of livestock and poultry and preventing diarrhea; inulinand galacto-oligosaccharides are added so that the absorption of the livestock and poultry on nutrient substances is promoted and the proliferation of intestinal probiotics is promoted; phytase is added so that the utilization rate on phytic acid in the feed of the livestock and poultry is increased, and the utilization rate of phosphorus salt and the emission of phosphorus are reduced; when themicro-ecological preparation provided by the invention is used, the disease-resisting capability of the livestock and poultry and the utilization capability on the feed are increased, and the growth speed is rapid.

Description

technical field [0001] The invention belongs to the technical field of probiotics industry, and in particular relates to a probiotics and its preparation method and application. Background technique [0002] The application of antibiotics in the field of animal husbandry has greatly promoted the development of animal husbandry scale and modernized farming. However, due to the abuse of antibiotics, various side effects have occurred, especially the residues of antibiotics in livestock and poultry products, which directly threaten Human health, potential hazards posed by livestock products. Since January 2006, the European Union has banned the use of antibiotics in animal feed. Every year, livestock and poultry products exported to Japan, the United States, and the European Union are restricted, returned or destroyed by various technical barriers. Among them, excessive drug residues are the main problem affecting the quality of my country's livestock and poultry products, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/18C12R1/23
CPCA23K10/18C12N1/16C12N1/20A23V2400/113Y02A40/70
Inventor 舒国伟梅莎上官文菲陈合黄丹
Owner SHAANXI UNIV OF SCI & TECH
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