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Stem blight bacteria of asparagus induced to secrete high content of cell wall degrading enzyme, screening method of cell wall degrading enzyme and stem blight resistant varieties

A technology for asparagus stem blight and asparagus stem blight, which is applied in the biological field, can solve the problems of defense enzymes and asparagus blight bacteria and the like, and achieves the effect of preventing asparagus stem blight

Active Publication Date: 2019-07-09
INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are few domestic and foreign research reports on the relationship between defense enzymes and Asparagus stem blight

Method used

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  • Stem blight bacteria of asparagus induced to secrete high content of cell wall degrading enzyme, screening method of cell wall degrading enzyme and stem blight resistant varieties
  • Stem blight bacteria of asparagus induced to secrete high content of cell wall degrading enzyme, screening method of cell wall degrading enzyme and stem blight resistant varieties
  • Stem blight bacteria of asparagus induced to secrete high content of cell wall degrading enzyme, screening method of cell wall degrading enzyme and stem blight resistant varieties

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Isolation and screening of pathogenic bacteria

[0048] Separation steps:

[0049] (1) Asparagus stem blight specimens were collected from 6 provinces including Jiangxi, Fujian, Hainan, Shandong, Shanxi, and Hebei, and stored in a 4-degree refrigerator for separation.

[0050] (2) Potato dextrose agar medium (potato dextrose agar medium) was prepared, after autoclaving at 121°C, pour 10 ml each into plates, cool down and set aside.

[0051] (3) Cut the specimen into a small piece of 5 mm × 5 mm at the junction of disease and health, put it in 75% alcohol for disinfection for 1 minute, transfer it to 0.1% mercury liter for disinfection for 30 seconds, and then transfer it to 75% alcohol for disinfection 1 minute, then transferred to sterile water to wash 2 times, and finally placed on absorbent paper to absorb the water.

[0052] (4) Transfer the small pieces that have been sucked dry to the potato dextrose agar medium plate, and then place them in an incubator at ...

Embodiment 2

[0068] Example 2 Comparing the Effects of Cell Wall Degrading Enzymes Induced by Various Pathogenic Bacteria

[0069] The pathogenic strains XT1, XT2, FJ2, FJ3 and FJ5 of Asparagus stem blight were all inoculated on asparagus stalks, and the types and quantities of induced cell wall degrading enzymes were compared. Table 1

[0070] Table 1 The activity of cell wall degrading enzymes induced by inoculation of several pathogenic strains of Asparagus stem blight on asparagus stalks (with 1% sodium carboxymethylcellulose as the induction substrate)

[0071]

[0072]

[0073]

[0074] Table 2 The activity of cell wall degrading enzymes induced by inoculation of several pathogenic strains of Asparagus stem blight on asparagus stalks (with 1% pectin as the induction substrate)

[0075]

[0076]

[0077]

Embodiment 3

[0078] Example 3 Variety Screening

[0079] (1) Inoculate Phomopsis asparagi with the preservation number: CGMCC No.13874 on asparagus stalks, and after 20 to 50 days of inoculation, investigate according to the 5-level grading standard listed below, the incidence:

[0080] The 5-level grading standard is as follows

[0081] Grade 0: no disease;

[0082] Grade 1: The diseased area accounts for less than 10% of the plane area where the lesion is located;

[0083] Grade 2: The diseased area accounts for 10.1% to 30% of the plane area where the lesion is located;

[0084] Grade 3: The diseased area accounts for 30.1% to 50% of the plane area where the lesion is located;

[0085] Grade 4: The diseased area accounts for 50.1% to 70% of the plane area where the lesion is located;

[0086] Grade 5: The diseased area accounts for more than 70% of the plane area where the lesion is located.

[0087] Table 3 Severity classification and symptom description of asparagus stem blight ...

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Abstract

The invention discloses a method for screening asparagus stem blight bacteria that induces and secretes high-content cell wall degrading enzymes, cell wall degrading enzymes and varieties resistant to asparagus stem blight, and belongs to the field of biotechnology. The asparagus stem blight bacteria induced to secrete high-content cell wall degrading enzymes provided by the present invention is Phomopsis asparagi, and the preservation number is: CGMCC No.13874. The asparagus stem blight fungus induced to secrete high-content cell wall degrading enzymes provided by the invention can induce asparagus to secrete high-content anti-cell wall degrading enzymes, and serves for the breeding of new disease-resistant varieties and the rational distribution of disease-resistant varieties.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to an asparagus stem blight fungus capable of inducing and secreting high-content defense enzymes. Background technique [0002] Asparagus (Asparagus officinalis L.) is a perennial herbaceous plant of Asparagus family Asparagus. It is fed with tender stems and has high nutritional and health value. It is rich in asparagine, saponins, flavonoids, selenium and plant polysaccharides, etc. The active ingredient has anti-tumor, anti-oxidation and hypolipidemic properties, and is known as the "King of Vegetables" and one of the "Top Ten Famous Dishes in the World" (Jaiswal et al., 2014; Nishimura et al., 2013). At the same time, the asparagus processing industry has a long chain, which can produce high value-added products such as asparagus anticancer drugs, asparagus tea, wine and beverages, and has broad prospects for application and development in the fields of food and medicine. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/42C12N9/26C12N9/18C12Q1/18A01G7/06C12R1/645
CPCA01G7/06C12N1/14C12N9/18C12N9/2408C12N9/2437C12Q1/18C12Y301/01011C12Y302/01004C12Y302/01015C12N1/145C12R2001/645G01N2333/37
Inventor 杨迎青兰波陈洪凡孙强周劲松黄嘉佳吴海燕李湘民
Owner INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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