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Efficient inducing method of anthurium andraeanum lind polyploid

A polyploid, anthurium technology, applied in the directions of botanical equipment and methods, applications, plant genetic improvement, etc.

Active Publication Date: 2018-06-22
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above problems cannot be effectively solved by traditional hybridization methods, and it is of urgent practical significance to carry out research on other breeding techniques

Method used

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  • Efficient inducing method of anthurium andraeanum lind polyploid
  • Efficient inducing method of anthurium andraeanum lind polyploid
  • Efficient inducing method of anthurium andraeanum lind polyploid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Take the callus of Anthurium cultivar 'Big Brother' and inoculate it in medium 1 / 2MS+1g / L Huabao No.1+2mg / L 6-BA+0.5mg / L NAA+0.5mg / L 2,4 -D+0.5mg / L TDZ+7g / L agar powder+30g / L sucrose pre-cultured for 15d ( figure 1 );

[0023] (2) Cut the callus into 0.5cm×0.5cm sized lumps and soak them in a 100mg / L colchicine solution for 5 days with shaking when the callus grows into raised growth points;

[0024] (3) After washing with sterile water, inoculate in differentiation medium 1 / 2MS+1g / L Huabao No. 1+2mg / L 6-BA+0.2mg / LNAA+7g / L agar powder+30g / L sucrose for differentiation to cultivate( figure 2 ); 45 days to count callus survival rate, survival rate=survival number / inoculation number×100%;

[0025] (4) Inoculate the differentiated seedlings into the rooting medium 1 / 2MS+0.2mg / L NAA+0.5g / L AC+7g / L agar powder+30g / L sucrose for rooting culture, subculture once every 30 days;

[0026] (5) After 3 subcultures, the rooted seedlings were transplanted to the plugs with 1:...

Embodiment 2

[0029] (1) Take the callus of Anthurium variety 'Big Brother' and inoculate it on the medium 1 / 2MS+1g / L Huabao No.1+3mg / L 6-BA+1mg / L NAA+2mg / L 2,4-D +1.0mg / L TDZ+7g / L agar powder+30g / L sucrose in pre-culture culture for 15 days;

[0030] (2) Cut the callus into 0.5cm×0.5cm-sized lumps and soak them in 200mg / L colchicine solution for 10 days with shaking when the callus grows out of convex growth points;

[0031] (3) After washing with sterile water, inoculate in differentiation medium 1 / 2MS+1g / L Huabao No. 1+2mg / L 6-BA+0.2mg / LNAA+7g / L agar powder+30g / L sucrose for differentiation Cultivate; count callus survival rate after 45 days, survival rate=survival number / inoculated number×100%;

[0032] (4) The differentiated seedlings were inoculated into rooting medium 1 / 2MS+0.2mg / L NAA+0.5g / L AC+7g / L agar powder+30g / L sucrose for rooting culture, subcultured once every 30 days;

[0033] (5) After 3 subcultures, the rooted seedlings were transplanted to plug-in trays equipped with 1...

Embodiment 3

[0036] (1) Take the callus of Anthurium cultivar 'Big Brother' and inoculate it in medium 1 / 2MS+1g / L Huabao No.1+2.5mg / L 6-BA+0.8mg / L NAA+1mg / L 2,4 Pre-culture in -D+0.8mg / L TDZ+7g / L agar powder+30g / L sucrose for 15 days;

[0037] (2) Cut the callus into 0.5cm×0.5cm-sized lumps and soak them in 400mg / L colchicine solution for 15 days with shaking when the callus grows into raised growth points;

[0038] (3) After washing with sterile water, inoculate in differentiation medium 1 / 2MS+1g / L Huabao No. 1+2mg / L 6-BA+0.2mg / LNAA+7g / L agar powder+30g / L sucrose for differentiation Cultivate; count callus survival rate after 45 days, survival rate=survival number / inoculated number×100%;

[0039] (4) The differentiated seedlings were inoculated into rooting medium 1 / 2MS+0.2mg / L NAA+0.5g / L AC+7g / L agar powder+30g / L sucrose for rooting culture, subcultured once every 30 days;

[0040] (5) After 3 subcultures, the rooted seedlings were transplanted to the plugs with 1:1:1 coco peat, vermic...

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Abstract

The invention discloses an efficient inducing method of anthurium andraeanum lind polyploidy. The method comprises the following steps: (1) callus tissue pre-culturing; (2) polyploidy inducing; (3) callus tissue differentiation culturing; (4) rooting culturing; and (5) domestication cultivating. The method disclosed by the invention is simple in operation, high in stability, high in inductivity, high in authentication efficiency and short in breeding period.

Description

technical field [0001] The invention belongs to the field of plant breeding and relates to a method for inducing polyploidy in plants, in particular to a method for efficiently inducing polyploidy in anthurium. Background technique [0002] Anthurium andraeanum Lind, also known as Anthurium, Anthurium, etc., is a perennial herbaceous plant of the genus Anthurium in the family Araceae. It is native to Colombia, Ecuador, Costa Rica and other places, and it likes warm and humid environments. . The advantages of unique flower shapes and various colors are deeply loved by people all over the world. In recent years, my country's anthurium industry has developed rapidly, and has become one of the world's main production and consumption places. [0003] The main means of creating new varieties of Anthurium are sexual hybridization and offspring selection. However, the traditional hybrid breeding cycle is relatively long, and the breeding of a variety usually takes 6 to 8 years,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 冷青云王存黄少华牛俊海
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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