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Application of 2,6-di-tert-butyl-p-cresol as plant disease-resistant activator in apple leaf part

A technology based on di-tert-butyl and p-cresol, applied in plant growth regulators, applications, biocides, etc., can solve the problems of difficulty in the control of apple leaf diseases, resistance to chemical pesticide residues, and high cost, and achieve elimination The destruction of the weak environment and the impact on human health, the effect of less resistance to drugs and low cost

Active Publication Date: 2018-06-15
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of difficult prevention and control of apple leaf diseases in current agricultural production, high cost, increasingly prominent chemical pesticide residues and drug resistance, etc., and provides a method for inducing disease resistance of apple leaves to effectively prevent and control apple leaf diseases

Method used

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  • Application of 2,6-di-tert-butyl-p-cresol as plant disease-resistant activator in apple leaf part
  • Application of 2,6-di-tert-butyl-p-cresol as plant disease-resistant activator in apple leaf part
  • Application of 2,6-di-tert-butyl-p-cresol as plant disease-resistant activator in apple leaf part

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1.1 Effect of 2,6-di-tert-butyl-p-cresol on mycelial growth of apple anthracnose leaf blight

[0027] Prepare PDA medium, add 2,6-di-tert-butyl-p-cresol aqueous solution to it, so that the final concentration is 0.05mmol L -1 , 0.1mmol·L -1 and 0.2mmol·L -1 , Inoculate the activated apple anthracnose leaf blight pathogen Glomerella cingulata into the above PDA medium. The bacterium is a conventional pathogenic bacterium in this field, and it was cultured at 25° C. in constant temperature and dark for 5 days, and the diameter of the colony was measured.

[0028] The above-mentioned PDA medium used has the following components and formula: weigh 200g after peeling the potatoes, cut into small pieces and boil in water for 15-20min, add 20g of glucose and 15g of agar powder after filtering through four layers of gauze, and dilute to 1000mL. The pH value is natural, and it is sterilized by high-pressure steam at 121°C for 20 minutes.

[0029] The results showed that 0.05...

Embodiment 2

[0035] Control effects of different concentrations of 2,6-di-tert-butyl-p-cresol on apple anthracnose leaf blight and brown spot:

[0036] Prepare the spore suspension of Glomerella cingulata, the pathogenic fungus of apple anthracnose leaf blight, and culture the pathogen on PDA at constant temperature and dark. , orange-yellow conidia horns can be seen after 2 to 3 days, prepare conidia suspension and adjust the concentration to 5×10 4 pcs mL -1 .

[0037] The spore suspension of Diplocarpon mali, the pathogen of apple brown spot disease, was prepared. The diseased leaves of brown spot disease were collected from the Jiaozhou Experimental Station of Qingdao Agricultural University. They were incubated at 20°C for 72 hours, and a large number of fresh conidia were produced on the diseased leaves. After that, make it into spore suspension 2~3×10 4 pcs mL -1 .

[0038] Configure 0.05mmol·L -1 , 0.1mmol·L -1 and 0.2mmol·L -1 The 2,6-di-tert-butyl-p-cresol solution, select ...

Embodiment 3

[0045] Control effect of 2,6-di-tert-butyl-p-cresol on apple brown spot and spotted leaf spot:

[0046] Prepare the spore suspension of Alternaria alternate f.sp.mali, the pathogenic fungus of spotted leaf defoliation, and inoculate it on the PDA medium, and cultivate it in a constant temperature incubator at 25°C. When the colonies turn black, Scrape off the hyphae and conidia, rinse with sterile water, filter the mixture through 3 layers of lens-cleaning paper to obtain a spore suspension and adjust the concentration to 2-3×10 5 pcs mL -1 .

[0047] Configure 0.1mmol·L -1 2,6-di-tert-butyl-p-cresol solution, select Fuji apple branches with consistent growth from one to two years with fully expanded leaves, and spray the above-mentioned concentration of 2,6-di-tert-butyl-p-cresol solution evenly until the leaves drip. , with sterile water as a control. After 3 days of treatment with the above-mentioned concentration of 2,6-di-tert-butyl-p-cresol solution, cut the treated ...

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Abstract

The invention discloses an application of 2,6-di-tert-butyl-p-cresol as a plant disease-resistant activator in an apple leaf part, belonging to the technical field of plant induced disease resistance.According to the invention, a 2,6-di-tert-butyl-p-cresol solution with a concentration of 0.05 mmol.L<-1> to 0.2 mmol.L<-1> can be used to induce the disease resistance of apple leaves and prevent and treat apple diseases. The 2,6-di-tert-butyl-p-cresol solution provided by the invention is free of in-vitro bactericidal or bacteriostatic effect, only can induce plant disease resistance under an in-vivo condition, induces generation of the disease resistance with characteristics of long effect duration and broad spectrum, is environment-friendly and safe, is not liable to generation of drug resistance, has simple application method, low concentration, less usage amount and low cost, and can improve the activity of defense enzymes and the expression level of genes related to disease resistance in a plant body.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance induction, and specifically relates to the application of 2,6-di-tert-butyl-p-cresol as a plant disease resistance activator. Background technique [0002] Apples are rich in carbohydrates, vitamins and inorganic salts, and are an important source of nutrition and material basis for people. As an important economic crop and export fruit in my country, apples have the largest planting area and output in the world. However, the export rate of apples is less than 3% of the total output, and the market share in the world is extremely disproportionate to my country's status as a major apple producing country. , apple disease is an important factor causing this phenomenon. [0003] Apple leaf diseases mainly include Diplocarpon mali, Glomerellacingulata, and Alternaria alternate f.sp.mali. Leaf diseases mainly cause leaves to turn yellow and fall off in large quantities in the early st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/08A01P3/00
CPCA01N31/08
Inventor 张清明王彩霞练森李保华其他发明人请求不公开姓名
Owner QINGDAO AGRI UNIV
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