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Rhodococcus ruber and application thereof in degradation of organic pollutants

A technology of organic pollutants and Rhodococcus rubrum, applied in the field of biodegradation of organic compounds, can solve problems such as high condition requirements and secondary pollution, and achieve the effect of broad application prospects

Active Publication Date: 2018-06-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the invention is to overcome the deficiencies in the prior art and provide a highly efficient Rhodococcus erythrococcus with the ability to degrade organic pollutants such as acetone and its application, which solves the problem that other technologies are prone to secondary pollution and conditions for degrading organic pollutants such as acetone. ask advanced questions

Method used

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  • Rhodococcus ruber and application thereof in degradation of organic pollutants
  • Rhodococcus ruber and application thereof in degradation of organic pollutants
  • Rhodococcus ruber and application thereof in degradation of organic pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Isolation, purification and identification of bacterial strains

[0023] 1. Isolation and purification of Rhodococcus ruber FQ-2.

[0024] Rhodococcus ruber (Rhodococcus ruber) FQ-2 is a fungus domesticated and isolated from the activated sludge in the aeration tank of a pharmaceutical factory in Zhejiang Province. Specific steps are as follows:

[0025] The activated sludge in the aeration tank of a pharmaceutical factory in Zhejiang Province was taken. After washing with tap water for five times, air-expose for 48 hours to remove residual organic matter as much as possible. Prepare the initial inorganic salt culture medium, inoculate the activated sludge, and use acetone with an initial concentration of 50 mg / L as the sole carbon source for directional domestication of the activated sludge. Replace the fresh inorganic salt culture medium every 3 days, and the separation can take about 40 days.

[0026] Initial inorganic salt medium composition: KH 2 ...

Embodiment 2

[0043] Example 2: Growth curve of Rhodococcus erythrococcus FQ-2.

[0044] After inoculating Rhodococcus erythrococcus FQ-2 into LB liquid medium, carry out shaking culture in a constant temperature shaker (30°C, 160r / min), and measure the OD of the bacterial solution every hour 600 , and draw the growth curve of the strain. LB liquid medium composition: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, solvent is deionized water, pH=7, sterilized at 121°C for 20min by high-pressure steam.

[0045] The growth curve of the bacteria measured by the experiment is as follows: image 3 shown. Rhodococcus Erythrococcus FQ-2 can continue to grow in LB medium and reach a stable period in about 12 hours, but after continuing to observe, it is found that it is still in a stable period after 24 hours. Generally, the stable period of bacterial growth is 8-12 hours. It is speculated that LB medium is used During the determination of the growth curve of the strain, the dead bacteria did not...

Embodiment 3

[0046] Example 3: Degradation of acetone by Rhodococcus rubella FQ-2.

[0047] Inoculate the Rhodococcus erythrococcus FQ-2 stored on the LB solid medium into the LB liquid medium, carry out shaking culture in a constant temperature shaker (30°C, 160r / min) for 12h (OD=0.7), and centrifuge at 8000rpm for 10 minutes , the obtained cells were inoculated into inorganic salt medium (initial OD 600 0.20), add 3 μL of acetone (concentration: 50 mg / L), carry out shaking culture in a constant temperature shaker (30°C, 160r / min), and measure the acetone concentration by gas chromatography at regular intervals. The experiment set up two parallel groups and a blank group (no bacterial solution).

[0048] LB liquid medium composition: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, solvent is deionized water, pH=7, sterilized at 121°C for 20min by high-pressure steam.

[0049] Composition of trace element mother liquor: CuSO 4 ·5H 2 O 0.02g·L -1 , FeSO 4 ·7H 2 O1.0g·L -1 , MnSO 4 4...

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Abstract

The invention discloses rhodococcus ruber FQ-2 and an application thereof in degradation of acetone and other common industrial organic pollutants. The application method comprises the steps: an inorganic salt culture medium containing acetone and other common industrial pollutants is inoculated with the rhodococcus ruber FQ-2, a degradation reaction is carried out under the conditions of the temperature of 30 DEG C and the rotating speed of 160 r / min, and thus the organic pollutants are degraded; the common organic pollutants comprise acetone, n-hexane, carbon disulfide, chlorobenzene, butylacetate, ethyl acetate or alpha-pinene. The rhodococcus ruber FQ-2 is taken from activated sludge of an aeration tank of a pharmaceutical factory in Zhejiang province, has good degradation effect on VOCs organic pollutants, especially acetone, and can completely convert acetone into harmless substances such as CO2, H2O, cell biomass and the like; at the same time, the bacterial strain also can degrade common industrial pollutants such as carbon disulfide and chlorobenzene in different extent, so the bacterial strain has broad application prospects in biological purification of industrial wastegas and wastewater.

Description

(1) Technical field [0001] The invention relates to a biodegradation technology of organic compounds, in particular to a strain of Rhodococcus ruber FQ-2 and its application in degrading common industrial organic pollutants such as acetone. (2) Background technology [0002] Acetone (molecular formula: CH 3 COCH 3 ) is a common organic solvent, a kind of volatile organic compounds (VOCs), widely used in production enterprises and industrial processes such as rubber, tanning and painting. Acetone is flammable and volatile, and its chemical properties are relatively active. It is irritating to the eyes, nose and throat. People exposed to this chemical for a long time can cause dizziness, burning sensation, pharyngitis, bronchitis, fatigue, irritability, etc. Therefore, it is urgent to find effective countermeasures to prevent and control acetone pollution, which is of great significance to human health and environmental protection. [0003] When using physical and chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/84B01D53/72B01D53/48B01D53/70C02F101/34C02F101/30C12R1/01
CPCB01D53/48B01D53/70B01D53/72B01D53/84B01D2251/95B01D2257/2064C02F3/34C02F2101/30C02F2101/34C12N1/205C12R2001/01Y02P20/59Y02A50/20
Inventor 成卓韦陈建孟於建明张晓敏
Owner ZHEJIANG UNIV OF TECH
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