A method for evaluating cell damage from smoke exposure using hyaluronic acid-coated gold nanoparticles

Abstract

The invention discloses a method for evaluating cell injuries caused by flue gas exposure through utilizing hyaluronic acid covered gold nanoparticles. The method comprises the following steps: A, sufficiently dispersing gold nanoparticles into a hyaluronic acid colloid water solution; then carrying out freezing and spray-drying on a mixture to obtain the dispersed gold nanoparticles with surfacescovered with hyaluronic acid hydrogel, so as to obtain the hyaluronic acid covered gold nanoparticles; B, enabling cells to be detected to take the hyaluronic acid covered gold nanoparticles; combining the taken hyaluronic acid covered gold nanoparticles with mRNA (messenger Ribonucleic Acid) by a catalysis hairpin assembling method, so as to carry out genomic hybridization through a complementary base pairing principle to form mRNA-DNA (Deoxyribonucleic Acid) which can emit fluorescent light; C, adding an electronic cigarette smoke extracting solution into a sample solution containing testedcells and then determining the intensity of the fluorescent light sent by the determined cells respectively before and after the solution is exposed into a flue gas capturing matter; determining theapoptosis rate according to the ratio of the sample solution to the electronic cigarette smoke extracting solution, so as to evaluate the injury degree, caused by the flue gas exposure, on the cells.

Description

technical field [0001] The invention belongs to the field of biological detection methods. Background technique [0002] When people smoke smoke from tobacco products, airway cells will be damaged to varying degrees due to direct exposure to smoke. The tobacco industry has been working hard to reduce the harm of tobacco in order to reduce this damage. Various harm reduction methods have been developed. In order to evaluate the effect of harm reduction, there needs to be a method for evaluating cell damage caused by smoke exposure, preferably a quantitative method. [0003] The traditional method is to take human airway cells (such as human alveolar type II epithelial cell line A549 cells) and expose them to smoke, and evaluate the damage degree of smoke exposure to cells by detecting the number of apoptotic cells after smoke exposure. In this method, it is necessary to use a fluorescent dye to stain the cellular DNA, and then analyze the number of living cells. However, f...

AI Technical Summary

Problems solved by technology

However, fluorescent dyes can destroy the activity of cells and lead to the production of intracellular toxins, which may interfere with the results of cell exposure to e-cigarette smoke and affect the accuracy of evaluation results

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