Preparation method of a transplantable scaffold for inducing circulating tumor cell metastasis

Abstract

The invention discloses a preparation method of an implantable stent capable of inducing metastasis of circulating tumor cells. Specifically, the preparation method comprises the following steps: spin-coating PDMS, so that a thin film is formed, and curing the thin film; conducting plasma treatment on the obtained PDMS film, so that the surface of the PDMS film is negatively charged; dropping a cationic polymer solution onto the surface of the PDMS film, implementing spin-coating, then dropping clean water and implementing spin-coating, then spraying a PBS solution containing an exosome, thendropping an anionic polymer solution, implementing spin-coating, then dropping clean water and implementing spin-coating, therefore through such circulation, a layer-by-layer self-assembled film containing the exosome is loaded on the PDMS surface; and removing the PDMS from a substrate, and implementing disinfecting via epoxy hexane gas. The implantable stent provided by the invention, as a flexible thin film, is excellent in denaturation capacity and mechanical performance, and implanting difficulty, caused by space limitation, can be overcome; and the PDMS is modified via a layer-by-layer self-assembling technology, so that biocompatibility, non-degradability and excellent deformation capacity and mechanical performance are reserved, and meanwhile, loading and sustained release of the exosome / drugs are achieved.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a method for preparing a transplantable scaffold for inducing the transfer of circulating tumor cells. Background technique [0002] Complications caused by metastasis of malignant tumors to functional organs (lung, liver, brain, etc.) are the main cause of tumor death. Circulating tumor cells (CTCs) are a type of tumor cells released from solid tumors or metastases into the peripheral blood circulation. Existing studies have proved that they play an important role in postoperative recurrence and distant metastasis of malignant tumors. However, the current mainstream tumor treatment methods, such as chemotherapy, radiotherapy, and surgical resection, are difficult to completely eliminate these circulating tumor cells. [0003] Exosomes are nanovesicles secreted by cells with a lipid bilayer structure, with a particle size distribution of 30-100 nm, and are widely dist...

AI Technical Summary

Problems solved by technology

However, the current scaffold materials that meet such requirements, such as silica gel materials, are relatively dense in structure and cannot be used for the loading and sustained release of exosomes and drugs, and the preparation process can easily inactivate biologically active molecules.

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