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Culture method of oudemansiella raphanipes by using three-dimensional layer frame

A cultivation method and technology of Gallus firsum, applied in culture medium, botany equipment and method, cultivation, etc., to achieve the effects of stable and uniform quality, convenient scientific management, and growth promotion

Inactive Publication Date: 2018-06-01
四川山水美地农业投资有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Based on the problems existing in the prior art, the object of the present invention is to provide a method for cultivating a three-dimensional layered shelf of Gallus fruticosa nigris that is simple in the cultivating process, high in success rate, low in cost and suitable for large-scale cultivation, to solve the problems of the prior art There is a vacancy in large-scale artificial culture technology of Gallium nigricans in China, so as to meet people's edible needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for cultivating a three-dimensional layer frame of Gallinarum nigella, the method for cultivating comprises the steps of:

[0019] Step 1): Prepare the bacteria bag, determine the composition and mass fraction ratio of the culture substrate raw materials, 50 parts of wood chips, 45 parts of DDS, 35 parts of cottonseed hulls, 3 parts of soybean meal, 5 parts of corn flour, 3 parts of wheat bran, 3 parts of lime, 8 parts of termite nest soil, add water and mix evenly, the water content is 50%, and make bacteria bag;

[0020] Step 2): Mycelium culture, after sterilization, cut the mycelium mother seed block containing termite secretion into 1.5cm pieces 3 , placed in the culture medium, cultivated at a constant temperature at 21±1°C and under light-shading conditions, cultivated for 18 days until the hyphae turn color and become physiologically mature, and enter the cultivation stage;

[0021] Step 3): Cultivation, evenly distribute the bacteria packs cultivated ...

Embodiment 2

[0024] A method for cultivating a three-dimensional layer frame of Gallinarum nigella, the method for cultivating comprises the steps of:

[0025] Step 1): Prepare the bacterial bag, determine the composition and mass fraction ratio of the culture substrate raw materials, 48 ​​parts of wood chips, 36 parts of DDS, 40 parts of cottonseed hulls, 4 parts of soybean meal, 6 parts of corn flour, 3 parts of wheat bran, 3 parts of lime, 10 parts of termite nest soil, add water and mix evenly, the water content is 50%, and make bacteria bag;

[0026] Step 2): Mycelium culture, after sterilization, cut the mycelium mother seed block containing termite secretion into 2cm pieces 3 , put it into the culture medium, and cultivate it at a constant temperature at 21±1°C under shading conditions, and cultivate it for 20 days until the hyphae turn color and become physiologically mature, and then enter the cultivation stage;

[0027] Step 3): Cultivation, evenly distribute the bacteria packs ...

Embodiment 3

[0030] A method for cultivating a three-dimensional layer frame of Gallinarum nigella, the method for cultivating comprises the steps of:

[0031] Step 1): Prepare the bacteria bag, determine the composition and mass fraction ratio of the culture substrate raw materials, 45 parts of wood chips, 32 parts of DDS, 38 parts of cottonseed hulls, 4 parts of soybean meal, 6 parts of corn flour, 3 parts of wheat bran, 4 parts of lime, 8 parts of termite nest soil, add water and mix evenly, the water content is 55%, and make bacteria bag;

[0032] Step 2): Mycelium culture, after sterilization, cut the mycelium mother seed block containing termite secretion into 2cm pieces 3 , put it into the culture medium, and cultivate it at a constant temperature at 21±1°C under shading conditions, and cultivate it for 20 days until the hyphae turn color and become physiologically mature, and then enter the cultivation stage;

[0033] Step 3): Cultivation, evenly distribute the bacteria packs cult...

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Abstract

The invention relates to a culture method of oudemansiella raphanipes by using a three-dimensional layer frame. The culture method comprises the following steps: 1) preparing a fungus bag, determininga culture medium raw material component and a mass fraction proportion, weighting the following raw materials: 40-50 parts of wood chips, 30-40 parts of DDS, 30-40 parts of cotton seed hull, 2-4 parts of soybean meal, 5-6 parts of corn flour, 2-3 parts of wheat bran, 3-4 parts of lime, and 5-10 parts of termitarium soil, adding water, and carrying out uniform mixing to prepare the fungus bags, wherein the water content is 40-60%; 2) culturing hyphae, after sterilization, cutting a hypha mother strain block containing termite secretion into the size of 1.5-2 cm<3>, putting the cut hypha motherstrain block into a culture medium, carrying out constant-temperature cultivation under a condition of 21+ / -1 DEG C and a light shielding condition, carrying out the cultivation for 15-20 days untilthe hypha color is changed, namely the physiological maturity is reached, and entering the a cultivation stage; 3) carrying out cultivation; and 4) carrying out harvesting, when the fungi grow to 5-8cm, harvesting the fungi. The culture method provided by the invention has the simple culture process, high success rate and low cost, and is suitable for large-scale culture.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a method for cultivating a three-dimensional layered frame of Gallus pilosulae. Background technique [0002] It is a kind of nutrient-rich edible fungus. It is rich in various amino acids. It is one of the edible fungi with both medicine and food. Chronic diarrhea is more than other symptoms, and it has the effect of inhibiting the growth of human cancer cells. Although the nutritional value of P. nigriscens is very high, it is not easy to cultivate artificially, because the requirements for the growth environment of P. nigriscens are extremely harsh, and the P. collusium does not reproduce itself, and the entire reproduction and growth cycle must rely on soil. Habitat termites gradually evolved into a symbiotic ecosystem with termites during the long-term evolution process. Termites spread the basidiospores and mycelium of Gallus spp., cultivate t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/62A01G24/20A01G24/12A01G24/25A01G24/23A01G24/22A01G24/10
CPCC05G3/00C05D3/02C05D9/00C05F5/002C05F5/008C05F11/00
Inventor 吴玲周善龙
Owner 四川山水美地农业投资有限公司
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