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Multi-channel microfluidic chip device for vacuum negative pressure sample injection, preparation method and application thereof

A microfluidic chip, vacuum negative pressure technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc. To achieve the effect of high-throughput accurate quantitative sensing, less sample consumption, and accurate quantitative sensing

Active Publication Date: 2018-05-29
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the above methods are limited by the complexity of the production process, the need for dilution of whole blood, and the need for pumps or other equipment to drive fluid manipulation, so they are not suitable for point-of-care diagnostic applications.
In addition, existing methods are deficient in plasma separation and subsequent analysis and detection docking, as well as accurate quantitative sensing of samples.

Method used

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  • Multi-channel microfluidic chip device for vacuum negative pressure sample injection, preparation method and application thereof
  • Multi-channel microfluidic chip device for vacuum negative pressure sample injection, preparation method and application thereof
  • Multi-channel microfluidic chip device for vacuum negative pressure sample injection, preparation method and application thereof

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Experimental program
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Embodiment 1

[0052] In this embodiment, the multi-channel microfluidic chip detection device for vacuum negative pressure sampling and blood filtration consists of two parts: a microfluidic chip and a microporous filter membrane. Such as figure 1 As shown, the microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a PDMS thin layer engraved with a microchannel structure and a PDMS thin layer without a microchannel.

[0053] The microchannel structure in the PDMS thin layer is a main channel connected vertically to eight parallel sample loading channels, and the structure of the entire microchannel is similar to a comb shape, as shown in figure 2 shown.

[0054] In this embodiment, the eight sample loading channels are located on the same side of the main channel, and the channel width and distance between channels are set to be the same.

[0055] The specific dimensions or data used in the description of the microchannel structure in this embodi...

Embodiment 2

[0058] The preparation method of the multi-channel microfluidic chip device for vacuum negative pressure sampling in the present invention comprises the following steps:

[0059] (1) Fabrication of PDMS microfluidic chip, the process can be found in Figure 4 , the specific production process can be divided into the following steps:

[0060] (1-1) Mask design and production: design the mask pattern of the microfluidic chip, and print the mask pattern on the film through a printer (resolution greater than 20000dpi) to obtain a film mask.

[0061] (1-2) Substrate cleaning and uniform glue: After the substrate is strictly cleaned with acetone, Piranha solution and ultrapure water and dried at high temperature (150℃×1h), add an appropriate amount of SU-8 photoresist dropwise in the center of the substrate (about 1ml / inch 2 ), put it on the turntable of the homogenizer, and adopt the program of 600rpm×18s, 2100rpm×60s (increase the speed at 100rpm / min) to homogenize the glue. Th...

Embodiment 3

[0078] A multi-channel microfluidic chip detection device for vacuum negative pressure sampling and blood filtration. The microfluidic chip is formed by bonding a polydimethylsiloxane (PDMS) thin layer carved with a microchannel and a PDMS thin layer without a microchannel. Make a crosscut on the two bonded PDMS to expose the main channel of the microchannel, apply a layer of glue around the exposed main channel opening at a certain distance, and stick a small piece of BTS film on the glue to make the main channel opening is sealed, thus forming the final chip device.

[0079] The microchannel structure is connected by an inlet channel and eight outlet channels, and its structural characteristics are as follows: the solution inlet channel (hereinafter referred to as the main channel) is a straight channel, which is connected with eight solution outlet channels (hereinafter referred to as the sample loading channel). The eight sample loading channels are arranged in parallel, ...

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Abstract

The invention relates to a multi-channel microfluidic chip detection device for vacuum negative pressure sample injection and a preparation method and application. The multi-channel microfluidic chipcomprises a main channel, a sample loading channel, a filter membrane, an optical transparent layer etched with a microchannel, and an optical transparent layer not etched with the microchannel; theoptical transparent layer etched with the microchannel is bonded and connected with the optical transparent layer not etched with the microchannel; the main channel and the sample loading channel arein negative pressure states; the filter membrane is located at the upper part of the main channel and bonded with the optical transparent layer etched with the microchannel and the optical transparentlayer not etched with the microchannel. The detection device disclosed by the invention has a small amount of samples and can realize automatic sample injection of the samples, 100% separation of blood cells and plasma of a whole blood sample, and seamless docking between the sample loading channel and a paper chip, so that separation and detection steps can be completed simultaneously.

Description

technical field [0001] The invention relates to a multi-channel microfluidic chip detection device for vacuum negative pressure sampling and blood filtration. Integrating blood cell filtration and accurate loading of whole blood samples, and providing a seamless docking interface for multi-channel paper chips, a key device for blood sample pretreatment is constructed, which has the potential to be widely used in the field of medical devices. Background technique [0002] Blood is the basic biomaterial component of the human body, consisting of plasma and blood cells. Plasma contains various nutrients such as plasma proteins (albumin, globulin and fibrinogen), lipoproteins, inorganic salts, oxygen, hormones, enzymes, antibodies and cell metabolites. Blood cells are composed of red blood cells, white blood cells and platelets. Physiological and pathological changes of the body often cause changes in blood components, so the detection of blood components has important clinica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/502761B01L2200/12
Inventor 冯晓均马朋刘笔锋
Owner HUAZHONG UNIV OF SCI & TECH
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