Application of C6 Aldehyde Volatile Substances in the Inhibition of Strawberry Coccidioides
A technology for volatile substances and strawberries, which is applied in the field of agricultural science to achieve the effect of reducing the amount of use and having obvious control effects.
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Embodiment 1
[0033] Determination of Main Volatile Substances in Embodiment 1 Strawberry Leaf
[0034] 1. Experimental materials
[0035] Fresh strawberry leaves are obtained from strawberry plants (Fragaria × ananassa, Albion), trade name "Albi".
[0036] 2. Experimental method
[0037] Headspace solid-phase microextraction enrichment-gas chromatography-mass spectrometry (GC-MS) was used to detect the main volatile substances and their contents in strawberry leaves.
[0038]Mix 20g of fresh strawberry leaves with saturated saline (1g:2.5mL) and homogenize, accurately weigh 3g of the sample and place it in a 15mL headspace bottle, add 1ul 300ppm, 2,6-dimethyl-6-heptane En-2-ol was used as an internal standard; after stirring and equilibrating at 40°C for 15 min, a 50 / 30 μm DVB / CAR / PDMS solid-phase microextraction head (Supelco, Bellefonte, PA, USA) was inserted for extraction for 30 min.
[0039] After the extraction, the sample was manually injected, and the sample port was analyzed at...
Embodiment 2
[0046] Inhibition of Main Volatile Substances in Example 2 Strawberry Leaves to Spore Germination and Mycelia Growth of Strawberry Coccomella
[0047] 1. Experimental materials
[0048] 1) Pathogens tested
[0049] M.fragariae, collected from strawberry plants with natural leaf snake eye disease, was isolated, purified, and identified in the laboratory and stored for later use. The spores used in this case were cultured within four generations of strawberry leaf medium.
[0050] 2) Test drug
[0051] Hexanal, trans-2-hexenal (Sigma-Aldrich, content greater than 98%), cis-3-hexenal (Sigma-Aldrich, 50% stabilized in triacetin)
[0052] 2. Experimental method
[0053] Set the concentration to 5 x 10 4 100uL of M.fragariae spore suspension per mL was evenly spread on the PDA medium (7mL medium per plate, about 0.15cm thick, and the diameter of the petri dish was 90mm), and stood for 10min to allow the spores to stick attached to the surface of the medium;
[0054] Place a 90...
Embodiment 3C6
[0076] The influence of embodiment 3C6 aldehydes volatile substances on the membrane potential of strawberry cocci (M.fragariae)
[0077] 1. Experimental materials
[0078] 1) Pathogens tested
[0079] M.fragariae, same as implementation case 2.
[0080] 2. Experimental method
[0081] 1) Set the concentration to 5×10 4 100uL of M.fragariae spore suspension per mL was evenly spread on the PDA medium (20mL medium per dish, about 0.15cm thick, and the diameter of the petri dish was 90mm). After cultivating for 5 days, follow the implementation The method in Case 2 was fumigated with C6 aldehyde volatile substances for 16 hours.
[0082] After the treatment, the hyphae were picked from each group, and the mitochondrial membrane potential of the mycelium was detected by using the mitochondrial membrane potential detection kit (JC-1) (Beyond Biotechnology, Shanghai).
[0083] The specific operation was performed according to the experimental procedure provided in the kit, and ...
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